Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 779,359 2 bp→G intergenic (+44486/+42473) unknown → / ← unknown tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1779,3590T.100.0% 97.6 / NA 23intergenic (+44486/+42474)unknown/unknowntRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (11/12);  total (11/12)
*1779,3600TG100.0% 73.8 / NA 23intergenic (+44487/+42473)unknown/unknowntRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/12);  total (11/12)

TAAAAGCTGTTGTTCCGCTTCTTCCATCCAAACTACGGTGTAATCCAATTTCATTTTAAGCCCAATACTTTTTCCAGGTTAGCTAATACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGTTTTGTATAATCT  >  1/779164‑779551
                                                                                                                                                                                                   |                                                                                                                                                                                                 
tAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGGCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATc                                                                                                                                           <  1:277438/251‑1 (MQ=255)
      ctgctgctCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCa                                                                                                                                                >  1:473074/8‑240 (MQ=255)
             tCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCg                                                                                                                              <  1:859939/251‑1 (MQ=255)
               cGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATc                                                                                                                                           >  1:889129/1‑236 (MQ=255)
                       ccgtccaAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAg                                                                                                                    <  1:500328/248‑1 (MQ=255)
                          tccaAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCaa                                                                                                                 <  1:716382/251‑1 (MQ=255)
                             aaaaaacgaactttttctgtttCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACt                                                                                                               <  1:723304/231‑1 (MQ=255)
                                     gtgtAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGAt                                                                                                                                     >  1:624756/1‑220 (MQ=255)
                                     gtgtAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAAc                                                                                                        >  1:69249/1‑249 (MQ=255)
                                     gtgtAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACAt                                                                                                      >  1:284637/1‑251 (MQ=255)
                                          aTCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAata                                                                                                 <  1:94911/251‑1 (MQ=255)
                                                    aTTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAg                                                                                       <  1:587518/251‑1 (MQ=255)
                                                           gtccAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTaag                                                                                 >  1:752043/3‑249 (MQ=255)
                                                                           aGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATaaa                                                                         <  1:654542/242‑1 (MQ=255)
                                                                               tAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGacaac                                                                                                                         >  1:734837/1‑190 (MQ=255)
                                                                                      caCTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGCTAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTAtgcg                                                     >  1:441258/2‑249 (MQ=255)
                                                                                        cttcttCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCaa                                                                                                                 >  1:779827/1‑189 (MQ=255)
                                                                                         ttcttcATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAAc                                                                                                        <  1:651000/197‑1 (MQ=255)
                                                                                             tcATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAAc                                                                                                        >  1:298682/1‑193 (MQ=255)
                                                                                                         aCTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGAt                                                                                                                                     <  1:715891/152‑1 (MQ=255)
                                                                                                                        ttATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATc                                                                                                                                           >  1:736513/1‑131 (MQ=255)
                                                                                                                                    gCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTaagca                                                                               <  1:404797/179‑4 (MQ=255)
                                                                                                                                         aaacaatacttcttCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCt  <  1:737277/243‑1 (MQ=255)
                                                                                                                                                                                                   |                                                                                                                                                                                                 
TAAAAGCTGTTGTTCCGCTTCTTCCATCCAAACTACGGTGTAATCCAATTTCATTTTAAGCCCAATACTTTTTCCAGGTTAGCTAATACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGTTTTGTATAATCT  >  1/779164‑779551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑