Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	778,467	2 bp→CA	intergenic (+43594/+43365)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	778,467	0	A	C	100.0%	147.3 / NA	43	intergenic (+43594/+43366)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/16);  total (27/16)
*	1	778,468	0	G	A	100.0%	149.9 / NA	43	intergenic (+43595/+43365)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/16);  total (27/16)

CACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGTGCCAATATCAGTACATATCAGAAAGACCTATTAAAAAATAATGTCAATATAACTTTCAGGAAAGGAGTTAAAAATGAAAAAGCCCTTTAAAATTGGTGTAATCTTTCTTGCTTTATGCAGTATGAGCCAAATAAGCCATGCGGTGGATGAATTAACCGGTGATACCAAAACCGCCTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCG  >  1/778236‑778688                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                             cacCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAaat                                                                                                                                                                                                            >  1:119104/1‑251 (MQ=255)               gTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAatatta                                                                                                                                                                                               >  1:58620/1‑248 (MQ=255)                  ccAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACtt                                                                                                                                                                                            <  1:489913/250‑1 (MQ=255)                                  aTCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAaat                                                                                                                                                                                                            >  1:877183/1‑218 (MQ=255)                                  aTCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTaaa                                                                                                                                                                           >  1:500486/1‑251 (MQ=255)                                               aCCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGcc                                                                                                                                                               >  1:636335/1‑250 (MQ=255)                                               aCCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGcc                                                                                                                                                               >  1:513522/1‑250 (MQ=255)                                                      gTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTaaa                                                                                                                                                        >  1:477520/1‑251 (MQ=255)                                                                 tgtgAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgata                                                                                                                                            >  1:145910/1‑241 (MQ=255)                                                                 tgtgAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgata                                                                                                                                            >  1:156918/1‑241 (MQ=255)                                                                      aCCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgatatgt                                                                                                                                         >  1:6629/1‑236 (MQ=255)                                                                        cTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgatatgtcttg                                                                                                                                     >  1:354759/1‑234 (MQ=255)                                                                            gTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgatatgtcttgcct                                                                                                                                  <  1:674266/250‑21 (MQ=255)                                                                                aaGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATg                                                                                                                              >  1:416563/1‑250 (MQ=255)                                                                                aaGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGc                                                                                                                             <  1:543900/251‑1 (MQ=255)                                                                                     aTTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTAt                                                                                                                        <  1:792673/251‑1 (MQ=255)                                                                                           tgaatgaaACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                   <  1:587181/250‑1 (MQ=255)                                                                                                 aaaCAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaa                                                                                                             <  1:575032/250‑6 (MQ=255)                                                                                                      gAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaagtcatg                                                                                                       >  1:104668/1‑240 (MQ=255)                                                                                                       aaaaaGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGAGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaagtca                                                                                                         <  1:901850/248‑10 (MQ=255)                                                                                                                     tttCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTa                                                                                         >  1:132505/1‑251 (MQ=255)                                                                                                                          aCTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCgg                                                                                    >  1:850524/1‑251 (MQ=255)                                                                                                                               gcgcATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATa                                                                               >  1:179385/1‑251 (MQ=255)                                                                                                                                       tGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                   >  1:138341/1‑207 (MQ=255)                                                                                                                                           gATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGctt                                                                   >  1:416471/1‑249 (MQ=255)                                                                                                                                               gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                   <  1:103457/199‑1 (MQ=255)                                                                                                                                               gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggta                                                                                                              >  1:596469/1‑200 (MQ=255)                                                                                                                                               gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGc                                                                                                      >  1:671611/1‑212 (MQ=255)                                                                                                                                               gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGa                                                               >  1:314977/1‑251 (MQ=255)                                                                                                                                               gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGa                                                               >  1:210422/1‑251 (MQ=255)                                                                                                                                                      aaTCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAatat                                                                                                                                                                                                 >  1:95777/1‑114 (MQ=255)                                                                                                                                                      aaTCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATAcga                                                                            >  1:26376/1‑229 (MQ=255)                                                                                                                                                       aTCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTAc                                                       <  1:30571/251‑1 (MQ=255)                                                                                                                                                                   ccGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGGTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCACTTGTGAAGCTCTACTCTGCTTatcat                                           <  1:442918/251‑1 (MQ=255)                                                                                                                                                                     ggCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAga                                                                                                                                                                                       >  1:527276/1‑108 (MQ=255)                                                                                                                                                                             ccGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTatca                                            <  1:894063/240‑1 (MQ=255)                                                                                                                                                                                  gCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACgcgg                            >  1:825176/1‑251 (MQ=255)                                                                                                                                                                                     tataAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTAc                                                       <  1:88811/221‑1 (MQ=255)                                                                                                                                                                                               ggTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGc                                                                                                                             <  1:239120/141‑1 (MQ=255)                                                                                                                                                                                                       gCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCAt        <  1:168404/250‑1 (MQ=255)                                                                                                                                                                                                            tGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATccgccg  >  1:338557/1‑251 (MQ=255)                                                                                                                                                                                                              gggTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATg                                                                                              <  1:810093/157‑1 (MQ=255)                                                                                                                                                                                                              gggTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATg                                                                                              <  1:373705/157‑1 (MQ=255)                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                             CACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGTGCCAATATCAGTACATATCAGAAAGACCTATTAAAAAATAATGTCAATATAACTTTCAGGAAAGGAGTTAAAAATGAAAAAGCCCTTTAAAATTGGTGTAATCTTTCTTGCTTTATGCAGTATGAGCCAAATAAGCCATGCGGTGGATGAATTAACCGGTGATACCAAAACCGCCTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCG  >  1/778236‑778688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑