Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	689,431	2 bp→GT	intergenic (+198516/‑7276)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	689,431	0	A	G	100.0%	11.0 / NA	5	intergenic (+198516/‑7277)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/2);  total (3/2)
*	1	689,432	0	C	T	100.0%	10.3 / NA	5	intergenic (+198517/‑7276)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/2);  total (3/2)

CGGTAGGGCTAAATACAAAGACAGTGGAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGGATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACC  >  1/689411‑689671                     ||                                                                                                                                                                                                                                                cGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGgtg            >  1:901509/1‑251 (MQ=255)   gTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTa          >  1:239399/1‑251 (MQ=255)       ggCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAAttt      >  1:88441/1‑251 (MQ=255)       ggCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAAt        <  1:173696/249‑1 (MQ=255)             aTACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTAcc  <  1:91189/249‑1 (MQ=255)                     ||                                                                                                                                                                                                                                                CGGTAGGGCTAAATACAAAGACAGTGGAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGGATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACC  >  1/689411‑689671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑