Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,119,381	C→A	intergenic (+7783/+488)	unknown → / ← unknown	tRNA‑Cys‑GCA/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,119,381	0	C	A	100.0%	229.8 / NA	66	intergenic (+7783/+488)	unknown/unknown	tRNA‑Cys‑GCA/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/28);  total (38/28)

CGGAATGTCAGTACGTAGGCAAAGATCAGGAATGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCCGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTATTCCAACCGGAAACTATTCTATGGAA  >  1/2119148‑2119625                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     cGGAATGTCAGTACGTAGGCAAAGATCAGGAATGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGc                                                                                                                                                                                                                                     <  1:308957/251‑1 (MQ=255)             aCGTAGGCAAAGATCAGGAATGGTATGCCGAACGCATAAAACATGATTGTACGGAGTTTGCAGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGc                                                                                                                                                                                                                         <  1:769256/251‑1 (MQ=255)                  ggCAAAGATCAGGAGTGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCa                                                                                                                                                                                                                     >  1:549253/1‑250 (MQ=255)                  ggCAAAGATCAGGAATGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATg                                                                                                                                                                                                                                 >  1:656802/1‑238 (MQ=255)                  ggCAAAGATCAGGAATGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCa                                                                                                                                                                                                                     >  1:774651/1‑250 (MQ=255)                        gATCAGGAATGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGttt                                                                                                                                                                                                                                                  >  1:269078/1‑215 (MQ=255)                          tCAGGAATGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTatg                                                                                                                                                                                                            <  1:167725/251‑1 (MQ=255)                          tCAGGAATGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTatg                                                                                                                                                                                                            <  1:371984/251‑1 (MQ=255)                                aTGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCg                                                                                                                                                                                                                  >  1:137567/1‑239 (MQ=255)                                aTGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGAtt                                                                                                                                                                                                      >  1:337738/1‑251 (MQ=255)                                  ggTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGGGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGAttt                                                                                                                                                                                                     >  1:496313/1‑250 (MQ=255)                                           aaCGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCAc                                                                                                                                                                                           <  1:268370/251‑1 (MQ=255)                                                   aaaCATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAGTCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGa                                                                                                                                                                                   >  1:464788/1‑251 (MQ=255)                                                          aTTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAg                                                                                                                                                                            >  1:493007/1‑251 (MQ=255)                                                          aTTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAg                                                                                                                                                                            >  1:467253/1‑251 (MQ=255)                                                             gTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCa                                                                                                                                                                         >  1:639690/1‑251 (MQ=255)                                                             gTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCa                                                                                                                                                                         >  1:458750/1‑251 (MQ=255)                                                                  gAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACAtt                                                                                                                                                                    >  1:131986/1‑251 (MQ=255)                                                                  gAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACAtt                                                                                                                                                                    >  1:599354/1‑251 (MQ=255)                                                                          cGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCAc                                                                                                                                                            <  1:417038/251‑1 (MQ=255)                                                                                ccATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGa                                                                                                                                                                                                                                               <  1:503454/162‑1 (MQ=255)                                                                                       ggTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGt                                                                                                                                               >  1:297573/1‑251 (MQ=255)                                                                                       ggTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGt                                                                                                                                               >  1:869855/1‑251 (MQ=255)                                                                                                         gTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTAt                                                                                                                             >  1:27399/1‑251 (MQ=255)                                                                                                           gCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTAtt                                                                                                                            >  1:701073/1‑250 (MQ=255)                                                                                                            cATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTc                                                                                                                           <  1:493516/250‑1 (MQ=255)                                                                                                              ttGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCaa                                                                                                                                                                                                                                   >  1:175491/1‑144 (MQ=255)                                                                                                                  aaTATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCgg                                                                                                                     <  1:744984/250‑1 (MQ=255)                                                                                                                    tatCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGgtgt                                                                                                                  >  1:417774/1‑251 (MQ=255)                                                                                                                     atCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCgg                                                                                                                     >  1:873096/1‑247 (MQ=255)                                                                                                                         tAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGtt                                                                                                                 <  1:356555/247‑1 (MQ=255)                                                                                                                         tAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATG‑TGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGc                                                                                                            <  1:782250/251‑1 (MQ=255)                                                                                                                           ggTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCCCCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGc                                                                                                            >  1:435626/1‑250 (MQ=255)                                                                                                                           ggTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCAc                                                                                                                                                                                           >  1:487051/1‑171 (MQ=255)                                                                                                                                   caATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTatga                                                                                                                                                                                                           >  1:110114/1‑147 (MQ=255)                                                                                                                                   caATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTatga                                                                                                                                                                                                           <  1:154205/147‑1 (MQ=255)                                                                                                                                   caATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCa                                                                                                   >  1:65935/1‑251 (MQ=255)                                                                                                                                   caATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCa                                                                                                   >  1:28304/1‑251 (MQ=255)                                                                                                                                   caATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTG‑‑‑GTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATg                                                                                                                                    >  1:119785/1‑215 (MQ=255)                                                                                                                                     atatGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAac                                                                                                 <  1:418853/251‑1 (MQ=255)                                                                                                                                       atGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAacac                                                                                               <  1:485860/251‑1 (MQ=255)                                                                                                                                       atGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAacac                                                                                               <  1:342438/251‑1 (MQ=255)                                                                                                                                                 gTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAacac                                                                                               >  1:292625/1‑241 (MQ=255)                                                                                                                                                          cAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAacac                                                                                               >  1:618715/1‑232 (MQ=255)                                                                                                                                                                cGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGa                                                                       <  1:85111/250‑1 (MQ=255)                                                                                                                                                                           cATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCAcc                                                           <  1:681191/251‑1 (MQ=255)                                                                                                                                                                                 ggTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATAc                                                                                                                                                                              >  1:593216/1‑130 (MQ=255)                                                                                                                                                                                    gtgAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGaaaa                                                                                       >  1:803898/1‑214 (MQ=255)                                                                                                                                                                                       aGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGc                                                <  1:498637/250‑1 (MQ=255)                                                                                                                                                                                       aGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGc                                                <  1:377771/250‑1 (MQ=255)                                                                                                                                                                                             gAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTatgat                                                                                                                                                                                                          >  1:128005/1‑90 (MQ=255)                                                                                                                                                                                             gAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGa                                         >  1:882278/1‑251 (MQ=255)                                                                                                                                                                                                  tGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGa                                                                       >  1:636959/1‑216 (MQ=255)                                                                                                                                                                                                            aCGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGc                                                                                                            <  1:583866/169‑1 (MQ=255)                                                                                                                                                                                                            aCGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTAtt                          >  1:233554/1‑251 (MQ=255)                                                                                                                                                                                                            aCGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTAtt                          >  1:746098/1‑251 (MQ=255)                                                                                                                                                                                                             cGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCGCCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTATTc                         <  1:250385/251‑1 (MQ=255)                                                                                                                                                                                                             cGTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTATTc                         <  1:191802/251‑1 (MQ=255)                                                                                                                                                                                                              gTTAGGATCCTTTATTAATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTATTc                         >  1:307086/1‑250 (MQ=255)                                                                                                                                                                                                                         ttattaATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCAc                                                                                                                                                            <  1:182371/108‑1 (MQ=255)                                                                                                                                                                                                                         ttattaATGCGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTATTCCAACCGGaaa               >  1:36053/1‑249 (MQ=255)                                                                                                                                                                                                                                  cGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTATTCCAACCGGAAACTATTCTATgg    <  1:301836/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTATTCCAACCGGAAACTATTCTATgg    <  1:615158/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTATTCCAACCGGAAACTATTCTATg     <  1:332496/250‑1 (MQ=255)                                                                                                                                                                                                                                   gAAACGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTc                              <  1:840022/224‑1 (MQ=255)                                                                                                                                                                                                                                    aaaCGCAGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTATTCCAACCGGAAACTATTCTATGGaa  <  1:697670/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     CGGAATGTCAGTACGTAGGCAAAGATCAGGAATGGTATGCCGAACGCATTAAACATGATTGTACGGAGTTTGCCGTTTACCATAAAGGTGAAAAAATCACGGAAGTGCATTGGAATATCGTAGGTGAACACAATATGCGTAACGGTTTGATGGCAATTGCGGCAGCGAATCATGCCGGTGTGAGCATTGAAAATGCCTGCGCGACGTTAGGATCCTTTATTAATGCGAAACGCCGTTTGGAAGTGAAAGGCAATGTTCATGGCATCACCGTTTATGATGATTTCGCCCATCACCCCACCGAAATACAGGCAACATTGACCGCACTTCGCGATAAAGTCGGGCAAAATGCGCGTATTCTGGCGGTGTTAGAGCCGCGTTCCAACACCATGAAAATGGGGGTACATAAAGATGAAATCGCACCAGCGTTAAAGCTGGCCGATGCGGTATTTCTATTCCAACCGGAAACTATTCTATGGAA  >  1/2119148‑2119625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑