Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,062,767	A→G	intergenic (‑5732/+41229)	unknown ← / ← unknown	tRNA‑Lys‑TTT/tRNA‑Ser‑TGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,062,767	0	A	G	100.0%	224.1 / NA	66	intergenic (‑5732/+41229)	unknown/unknown	tRNA‑Lys‑TTT/tRNA‑Ser‑TGA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/38);  total (28/38)

ACAAGAAGAACATTAATGTTAAGGTTAATTTACTTGAAGTAGCATCCCACATTAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCAGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTG  >  1/2062525‑2063009                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   aCAAGAAGAACATTAATGTTAAGGTTAATTTACTTGAAGTAGCATCCCACATTAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAGTTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTc                                                                                                                                                                                                                                             <  1:236920/250‑1 (MQ=255)  cAAGAAGAACATTAATGTTAAGGTTAATTTACTTGAAGTAGCATCCCACATTAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCtt                                                                                                                                                                                                                                           <  1:369783/251‑1 (MQ=255)                aTGTTAAGGTTAATTTACTTGAAGTAGCATCCCACATTAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAAc                                                                                                                                                                                                                             <  1:341353/251‑1 (MQ=255)                       ggTTAATTTACTTGAAGTAGCATCCCACATTAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGc                                                                                                                                                                                                                        <  1:877430/249‑1 (MQ=255)                        gTTAATTTACTTGAAGTAGCATCCCACATTAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCAc                                                                                                                                                                                                                      <  1:119588/250‑1 (MQ=255)                        gTTAATTTACTTGAAGTAGCATCCCACATTAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCAc                                                                                                                                                                                                                      <  1:305357/250‑1 (MQ=255)                                     aaGTAGCATCCCACATTAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTAc                                                                                                                                                                                                            <  1:802662/247‑1 (MQ=255)                                               ccACATTAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTAc                                                                                                                                                                                              <  1:810754/251‑1 (MQ=255)                                                    ttAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAgttg                                                                                                                                                                                                                                   <  1:182794/209‑1 (MQ=255)                                                    ttAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTAcc                                                                                                                                                                                             >  1:760809/1‑247 (MQ=255)                                                    ttAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAAt                                                                                                                                                                                          >  1:82015/1‑250 (MQ=255)                                                           aCTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAAc                                                                                                                                                                                                                             >  1:710690/1‑208 (MQ=255)                                                                           gaAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTAc                                                                                                                                                                                                            >  1:467526/1‑209 (MQ=255)                                                                                      cATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTg                                                                                                                                                        <  1:114714/250‑1 (MQ=255)                                                                                        tCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTg                                                                                                                                                     <  1:623706/251‑1 (MQ=255)                                                                                        tCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTg                                                                                                                                                     >  1:177266/1‑251 (MQ=255)                                                                                        tCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTg                                                                                                                                                     <  1:472354/251‑1 (MQ=255)                                                                                         cacaAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGa                                                                                                                                                    <  1:207086/251‑1 (MQ=255)                                                                                                   aaCATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAAcc                                                                                                                                          <  1:254937/251‑1 (MQ=255)                                                                                                                    ggTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAAc                                                                                                                          >  1:607221/1‑250 (MQ=255)                                                                                                                    ggTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAAc                                                                                                                          >  1:462405/1‑250 (MQ=255)                                                                                                                        aaaaTCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATac                                                                                                                     <  1:186614/251‑1 (MQ=255)                                                                                                                         aaaTCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTAc                                                                                                                                                                                                            >  1:331973/1‑163 (MQ=255)                                                                                                                          aaTCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAg                                                                                                                                                                                                                                      <  1:657588/136‑1 (MQ=255)                                                                                                                          aaTCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATacac                                                                                                                   <  1:828531/251‑1 (MQ=255)                                                                                                                          aaTCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATacac                                                                                                                   <  1:415490/251‑1 (MQ=255)                                                                                                                          aaTCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATacac                                                                                                                   <  1:322225/251‑1 (MQ=255)                                                                                                                          aaTCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATacac                                                                                                                   <  1:556070/251‑1 (MQ=255)                                                                                                                                        tGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACAcc                                                                                                                  >  1:854796/1‑238 (MQ=255)                                                                                                                                         ggTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTc                                                                                                                                                                                                                                             >  1:608374/1‑114 (MQ=255)                                                                                                                                         ggTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCGCCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATacac                                                                                                                   <  1:634115/236‑1 (MQ=255)                                                                                                                                         ggTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAgg                                                                                                    >  1:360441/1‑251 (MQ=255)                                                                                                                                                gatgaGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTtg                                                                                                                                                                       <  1:391254/177‑1 (MQ=255)                                                                                                                                                 atgaGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAAt                                                                                            <  1:378997/251‑1 (MQ=255)                                                                                                                                                     gaACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAg                                                                                                                                                                                                                                      <  1:694694/109‑1 (MQ=255)                                                                                                                                                        cAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACgg                                                                                     >  1:334876/1‑251 (MQ=255)                                                                                                                                                         aagaagGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGt                                                                                    <  1:714953/251‑1 (MQ=255)                                                                                                                                                              ggCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTtg                                                                                                                                                                       >  1:659033/1‑163 (MQ=255)                                                                                                                                                              ggCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCacac                                                                                                                                                          <  1:215095/176‑1 (MQ=255)                                                                                                                                                              ggCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGca                                                                                >  1:633417/1‑250 (MQ=255)                                                                                                                                                               gCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGcac                                                                               <  1:511857/250‑1 (MQ=255)                                                                                                                                                                           cGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTAc                                                                                                                                                                                                            >  1:57079/1‑113 (MQ=255)                                                                                                                                                                                    ttCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATacac                                                                                                                   <  1:69641/193‑1 (MQ=255)                                                                                                                                                                                      cGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAgg                                                                                                    >  1:772734/1‑206 (MQ=255)                                                                                                                                                                                         gaATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCAt                                                                                                                       >  1:498179/1‑184 (MQ=255)                                                                                                                                                                                           atatTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCa                                                  >  1:849331/1‑251 (MQ=255)                                                                                                                                                                                                        cAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGa                                       <  1:498993/249‑1 (MQ=255)                                                                                                                                                                                                            gCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGt                                 >  1:431070/1‑251 (MQ=255)                                                                                                                                                                                                                 cccTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAAc                                                                                                                                           <  1:410526/140‑1 (MQ=255)                                                                                                                                                                                                                 cccTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATacac                                                                                                                   <  1:216467/164‑1 (MQ=255)                                                                                                                                                                                                                 cccTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGc                                               >  1:16273/1‑232 (MQ=255)                                                                                                                                                                                                                 cccTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCa                            >  1:822480/1‑251 (MQ=255)                                                                                                                                                                                                                 cccTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCa                            >  1:758123/1‑251 (MQ=255)                                                                                                                                                                                                                   cTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCAtt                          <  1:284045/251‑1 (MQ=255)                                                                                                                                                                                                                       aGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATc                                                                                                                                       >  1:53608/1‑138 (MQ=255)                                                                                                                                                                                                                        gCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAg                     >  1:116892/1‑251 (MQ=255)                                                                                                                                                                                                                         ccAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATacac                                                                                                                   >  1:281485/1‑156 (MQ=255)                                                                                                                                                                                                                            aTACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCAAACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCAtt                          >  1:586498/1‑242 (MQ=255)                                                                                                                                                                                                                                    aaTAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCAcc         <  1:750636/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaTAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCAc          <  1:151473/250‑1 (MQ=255)                                                                                                                                                                                                                                         ccATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATggg    <  1:124325/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATggg    >  1:71303/1‑251 (MQ=255)                                                                                                                                                                                                                                         ccATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATggg    <  1:685616/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATggg    <  1:112107/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGATCGCACCATggg    >  1:613414/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGggtg  <  1:623220/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   ACAAGAAGAACATTAATGTTAAGGTTAATTTACTTGAAGTAGCATCCCACATTAATAAACTCATTTCCGGATGAGAAATGGATGGCATCACAAATGGGAACATAGCAATTGCAGCGGTTAAAATCACACCGGCCATGGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCAGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTG  >  1/2062525‑2063009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑