Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,053,948	A→G	intergenic (+53781/+2808)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,053,948	0	A	G	100.0%	229.7 / NA	68	intergenic (+53781/+2808)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/43);  total (25/43)

GAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGAAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAAC  >  1/2053706‑2054188                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 gAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGt                                                                                                                                                                                                                                           <  1:252549/250‑1 (MQ=255)     gTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGAAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGa                                                                                                                                                                                                                                      >  1:800790/1‑251 (MQ=255)       ccGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGAtt                                                                                                                                                                                                                                    <  1:151457/251‑1 (MQ=255)          aTGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCgg                                                                                                                                                                                                                                 >  1:66252/1‑251 (MQ=255)                        cAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACg                                                                                                                                                                                                                   >  1:48057/1‑251 (MQ=255)                         aaaTAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACg                                                                                                                                                                                                                   >  1:764899/1‑250 (MQ=255)                              atCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGt                                                                                                                                                                                                             >  1:12777/1‑251 (MQ=255)                                      ccGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGAc                                                                                                                                                                                                      <  1:750877/250‑1 (MQ=255)                                        gACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCATCCAACGCATTGTTCATGACAt                                                                                                                                                                                                    >  1:15892/1‑250 (MQ=255)                                                ggCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTg                                                                                                                                                                                                 <  1:461484/245‑1 (MQ=255)                                                  cTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACcaca                                                                                                                                                                                         <  1:267484/251‑1 (MQ=255)                                                     cAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATcc                                                                                                                                                                                      <  1:206365/251‑1 (MQ=255)                                                           aCAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAAc                                                                                                                                                                                                                    >  1:497950/1‑215 (MQ=255)                                                           aCAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGcc                                                                                                                                                                                 >  1:248450/1‑250 (MQ=255)                                                              gATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGAc                                                                                                                                                                                                      >  1:93721/1‑226 (MQ=255)                                                                  aCGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTtcc                                                                                                                                                                                                                          >  1:312832/1‑202 (MQ=255)                                                                     aTCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATcc                                                                                                                                                                                      >  1:74318/1‑235 (MQ=255)                                                                        gTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGAt                                                                                                                                                                                                                               <  1:88655/191‑1 (MQ=255)                                                                            tcaCGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATg                                                                                                                                                               <  1:794054/251‑1 (MQ=255)                                                                               cgacgGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCa                                                                                                                                                             >  1:259187/1‑250 (MQ=255)                                                                                    ggCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGccc                                                                                                                                                                                >  1:267651/1‑226 (MQ=255)                                                                                          aTCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAAc                                                                                                                                                  <  1:579490/250‑1 (MQ=255)                                                                                          aTCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACt                                                                                                                                                 <  1:253501/251‑1 (MQ=255)                                                                                           tCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTg                                                                                                                                                <  1:586312/251‑1 (MQ=255)                                                                                                            atatTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACa                                                                                                                                                                                                     <  1:521065/181‑1 (MQ=255)                                                                                                            atatTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGc                                                                                                                                                                                  <  1:26919/200‑1 (MQ=255)                                                                                                             tatTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCGCCTCCGCTAACTGTTCGGCAAATTGCTGGAt                                                                                                                              <  1:581962/251‑1 (MQ=255)                                                                                                             tatTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGAt                                                                                                                              <  1:375007/251‑1 (MQ=255)                                                                                                                 gCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCAc                                                                                                                           >  1:409057/1‑250 (MQ=255)                                                                                                                   aTTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACctc                                                                                                                        <  1:14748/251‑1 (MQ=255)                                                                                                                        aTCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                   <  1:617525/251‑1 (MQ=255)                                                                                                                        aTCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                   <  1:786969/251‑1 (MQ=255)                                                                                                                            aaaCTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAatat                                                                                                                <  1:821291/250‑1 (MQ=255)                                                                                                                            aaaCTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAAtata                                                                                                               >  1:534565/1‑251 (MQ=255)                                                                                                                              aCTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                   >  1:727643/1‑245 (MQ=255)                                                                                                                                tGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                           <  1:777041/251‑1 (MQ=255)                                                                                                                                tGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                           <  1:322303/251‑1 (MQ=255)                                                                                                                                tGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                           <  1:811508/251‑1 (MQ=255)                                                                                                                                 gTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAAc                                                                                                                                                  >  1:304761/1‑211 (MQ=255)                                                                                                                                      gcgcTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGt                                                                                                      <  1:845299/250‑1 (MQ=255)                                                                                                                                       cgcTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGATCGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAATGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGtt                                                                                                     <  1:381713/250‑1 (MQ=255)                                                                                                                                                     ggTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                   >  1:670424/1‑222 (MQ=255)                                                                                                                                                     ggTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                           <  1:397597/230‑1 (MQ=255)                                                                                                                                                      gTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTc                                                                                                                                                         <  1:21720/183‑1 (MQ=255)                                                                                                                                                      gTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAatat                                                                                                                <  1:209463/224‑1 (MQ=255)                                                                                                                                                       tATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                           <  1:271996/228‑1 (MQ=255)                                                                                                                                                                tcgtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGagaa                                                                           >  1:578325/1‑251 (MQ=255)                                                                                                                                                                 cgtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGagaag                                                                          >  1:205563/1‑251 (MQ=255)                                                                                                                                                                 cgtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCAGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCa                                                                                                                                                                                     >  1:565006/1‑144 (MQ=255)                                                                                                                                                                  gtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGt                                                                                                      <  1:427831/222‑1 (MQ=255)                                                                                                                                                                         ccGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATc                                                                  <  1:66918/251‑1 (MQ=255)                                                                                                                                                                         ccGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATc                                                                  <  1:557273/251‑1 (MQ=255)                                                                                                                                                                                   aaaCTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGAt                                                                                                                              <  1:379379/181‑1 (MQ=255)                                                                                                                                                                                        actCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTaa                                                   >  1:801674/1‑251 (MQ=255)                                                                                                                                                                                            tGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGc                                               <  1:871585/251‑1 (MQ=255)                                                                                                                                                                                              aaCGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTg                                             <  1:479328/251‑1 (MQ=255)                                                                                                                                                                                                 ggAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAAt                                                 >  1:300040/1‑244 (MQ=255)                                                                                                                                                                                                  gAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGAc                                                                                                                                                                                                      <  1:753708/94‑1 (MQ=255)                                                                                                                                                                                                  gAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAAc                                                                                                                                                  >  1:414319/1‑146 (MQ=255)                                                                                                                                                                                                  gAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                           >  1:102567/1‑185 (MQ=255)                                                                                                                                                                                                  gAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTg                                             <  1:752146/247‑1 (MQ=255)                                                                                                                                                                                                        agCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCAtt                                     <  1:761445/249‑1 (MQ=255)                                                                                                                                                                                                          cgcATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTc                                 <  1:791672/251‑1 (MQ=255)                                                                                                                                                                                                          cgcATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTc                                 <  1:743213/251‑1 (MQ=255)                                                                                                                                                                                                                 gcagcaATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCAcc                                                                                                                                                           <  1:707211/122‑1 (MQ=255)                                                                                                                                                                                                                       aTGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACgg                    <  1:592100/251‑1 (MQ=255)                                                                                                                                                                                                                                         tGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAAc  <  1:689372/251‑1 (MQ=255)                                                                                                                                                                                                                                         tGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAAc  <  1:697711/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGAAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAAC  >  1/2053706‑2054188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑