Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,031,329	C→T	intergenic (+31162/+25427)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,031,329	0	C	T	100.0%	108.6 / NA	33	intergenic (+31162/+25427)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/16);  total (17/16)

CCGATTTGTTGGGCATAAAGATCAACAGCAACCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGCTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATAAAATTCCTTGATAATCAATATATCGAATTTGACGGCAAAGTAACAAAATTT  >  1/2031087‑2031564                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            ccGATTTGTTGGGCATAAAGATCAACAGCAACCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTg                                                                                                                                                                                                                                      <  1:128079/250‑1 (MQ=255)                    gATCAACAGCAACCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTACATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATa                                                                                                                                                                                                                  >  1:219166/1‑251 (MQ=255)                     aTCAACAGCAACCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACAt                                                                                                                                                                                                                   <  1:650499/249‑1 (MQ=255)                              aaCCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGaaa                                                                                                                                                                                                        >  1:22882/1‑251 (MQ=255)                                       aaGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATc                                                                                                                                                                                               <  1:820335/251‑1 (MQ=255)                                                  ggTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCaaa                                                                                                                                                                                    >  1:614643/1‑251 (MQ=255)                                                                    ccGTCTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAAcc                                                                                                                                                                                                                                        >  1:204036/1‑181 (MQ=255)                                                                                tGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATttaaa                                                                                                                                                      >  1:115403/1‑251 (MQ=255)                                                                                                     tGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATAc                                                                                                                                 <  1:276290/251‑1 (MQ=255)                                                                                                       aaaTTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTtatata                                                                                                                                  >  1:100147/1‑248 (MQ=255)                                                                                                           tattTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATAc                                                                                                                                 >  1:150893/1‑245 (MQ=255)                                                                                                               ttACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCaa                                                                                                                       <  1:685534/251‑1 (MQ=255)                                                                                                                tACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCaa                                                                                                                                                                                     <  1:868973/188‑1 (MQ=255)                                                                                                                     gCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACAt                                                                                                                                                                                                                   >  1:664545/1‑153 (MQ=255)                                                                                                                                  aCATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTTAATGCTCGCTACCCGTAAAgg                                                                                                    <  1:263276/251‑1 (MQ=255)                                                                                                                                   cATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCt                                                                                                                                        <  1:589064/214‑1 (MQ=255)                                                                                                                                                aaaaTCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAAcc                                                                                                                                                                                                                                        >  1:365853/1‑105 (MQ=255)                                                                                                                                                       aCATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTaa                                                                               >  1:393627/1‑251 (MQ=255)                                                                                                                                                            gAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAGAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAg                                                                                                                                              >  1:488816/1‑183 (MQ=255)                                                                                                                                                                 ttGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGt                                                                     <  1:3586/251‑1 (MQ=255)                                                                                                                                                                       atgtgtataaagagacagaaaTCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGcac                                                                <  1:234885/232‑1 (MQ=255)                                                                                                                                                                               gTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATc                                                                                                                                                                                               <  1:360797/115‑1 (MQ=255)                                                                                                                                                                                       caAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATaa                                                    >  1:511141/1‑246 (MQ=255)                                                                                                                                                                                       caAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATaa                                                    >  1:322295/1‑246 (MQ=255)                                                                                                                                                                                                  ccGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGaa                                                                                                                                            <  1:98056/147‑1 (MQ=255)                                                                                                                                                                                                                  tctATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATaaaa                                                  <  1:826514/221‑1 (MQ=255)                                                                                                                                                                                                                     atatTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATc                                                                                                                                                                                               <  1:256623/77‑1 (MQ=255)                                                                                                                                                                                                                     atatTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAg                                                                                                                                                              >  1:42166/1‑110 (MQ=255)                                                                                                                                                                                                                     atatTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAg                                                                                                                                              >  1:77459/1‑126 (MQ=255)                                                                                                                                                                                                                                    aGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATAAAATTCCTTGATAATCAATATATCGAATTTGACGGCAAAGTAACAAAAttt  >  1:681000/1‑251 (MQ=255)                                                                                                                                                                                                                                     gATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAgg                                                                                                    <  1:166635/152‑1 (MQ=255)                                                                                                                                                                                                                                     gATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGAc                                                                            >  1:820905/1‑176 (MQ=255)                                                                                                                                                                                                                                     gATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATAAAAt                                                 <  1:554534/203‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            CCGATTTGTTGGGCATAAAGATCAACAGCAACCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGCTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATAAAATTCCTTGATAATCAATATATCGAATTTGACGGCAAAGTAACAAAATTT  >  1/2031087‑2031564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑