Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,017,066	G→A	intergenic (+16899/+39690)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,017,066	0	G	A	100.0%	231.7 / NA	66	intergenic (+16899/+39690)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/30);  total (36/30)

GACATCGCCCGTATGCTCGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCGCTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAGGGTCAACGCCGAGCATATTTAATCCTTTATTGGTTGTACC  >  1/2016820‑2017303                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              gACATCGCCCGTATGCTCGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTa                                                                                                                                                                                                                                           >  1:304341/1‑251 (MQ=255) gACATCGCCCGTATGCTCGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTa                                                                                                                                                                                                                                           >  1:882298/1‑251 (MQ=255) gACATCGCCCGTATGCTCGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTa                                                                                                                                                                                                                                           >  1:398744/1‑251 (MQ=255)    aTCGCCCGTATGCTCGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTAttt                                                                                                                                                                                                                                        <  1:190900/251‑1 (MQ=255)        cccGTATGCTCGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTgg                                                                                                                                                                                                                                     <  1:558229/250‑1 (MQ=255)        cccGTATGCTCGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTgg                                                                                                                                                                                                                                     <  1:900784/250‑1 (MQ=255)          cGTATGCTCGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTa                                                                                                                                                                                                                                  >  1:660803/1‑251 (MQ=255)                cTCGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATc                                                                                                                                                                                                                             <  1:236584/250‑1 (MQ=255)                  cGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCaa                                                                                                                                                                                                                          >  1:846953/1‑251 (MQ=255)                        gccttgcAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGatt                                                                                                                                                                                                                    >  1:103568/1‑251 (MQ=255)                        gccttgcAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGatt                                                                                                                                                                                                                    >  1:88536/1‑251 (MQ=255)                             gcagcaCACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTAttt                                                                                                                                                                                                                                        <  1:197706/226‑1 (MQ=255)                                     ccGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGGAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTAtttt                                                                                                                                                                                                                                       <  1:638015/219‑1 (MQ=255)                                        gaagaaGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTtgt                                                                                                                                                                                                    >  1:25967/1‑251 (MQ=255)                                        gaagaaGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTtgt                                                                                                                                                                                                    >  1:784838/1‑251 (MQ=255)                                            aagGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGt                                                                                                                                                                                                <  1:797459/251‑1 (MQ=255)                                                        cTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGAttatta                                                                                                                                                                                                                <  1:158091/223‑1 (MQ=255)                                                        cTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAg                                                                                                                                                                                    >  1:221671/1‑251 (MQ=255)                                                                  aGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAata                                                                                                                                                                          >  1:4994/1‑251 (MQ=255)                                                                           cGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAgg                                                                                                                                                                 >  1:822952/1‑251 (MQ=255)                                                                             aTTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGAt                                                                                                                                                                                                                              >  1:394113/1‑188 (MQ=255)                                                                             aTTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATc                                                                                                                                                                                                                             >  1:781699/1‑189 (MQ=255)                                                                             aTTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGc                                                                                                                                                                <  1:705693/250‑1 (MQ=255)                                                                                tAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGc                                                                                                                                                                <  1:860270/247‑1 (MQ=255)                                                                                               gCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATAttt                                                                                                                                                                       <  1:673112/225‑1 (MQ=255)                                                                                               gCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGc                                                                                                                                                                <  1:121558/232‑1 (MQ=255)                                                                                                 aTTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAg                                                                                                                                           <  1:394645/251‑1 (MQ=255)                                                                                                         gTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCa                                                                                                                                   >  1:210611/1‑251 (MQ=255)                                                                                                              tCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCaaaaaa                                                                                                                              >  1:500369/1‑251 (MQ=255)                                                                                                                     aCAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATcc                                                                                                                       <  1:402260/251‑1 (MQ=255)                                                                                                                          cgtcttatggcttggCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCttttt                                                                                                                  >  1:284303/15‑251 (MQ=255)                                                                                                                                aTGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTc                                                                                                                                                                     <  1:133706/194‑1 (MQ=255)                                                                                                                                aTGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTacga                                                                                                             >  1:543868/1‑250 (MQ=255)                                                                                                                                        gCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAACGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAg                                                                                                    >  1:426447/1‑251 (MQ=255)                                                                                                                                        gCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAg                                                                                                    >  1:97503/1‑251 (MQ=255)                                                                                                                                         cGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAgg                                                                                                   <  1:234632/251‑1 (MQ=255)                                                                                                                                            ggatggatGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTAt                                                                                                >  1:228168/1‑251 (MQ=255)                                                                                                                                                     cccGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATa                                                                                       >  1:134622/1‑251 (MQ=255)                                                                                                                                                     cccGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATa                                                                                       >  1:414456/1‑251 (MQ=255)                                                                                                                                                      ccGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTa                                                                                                                <  1:799763/225‑1 (MQ=255)                                                                                                                                                           gtGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGa                                                                                 >  1:821434/1‑251 (MQ=255)                                                                                                                                                              aTGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAgg                                                                                                                                                                                                                       <  1:488935/114‑1 (MQ=255)                                                                                                                                                              aTGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGaaa                                                                                                                                                                            >  1:81454/1‑157 (MQ=255)                                                                                                                                                              aTGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACaa                                                                              >  1:692317/1‑251 (MQ=255)                                                                                                                                                                gtgGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACg                                                                            >  1:403561/1‑251 (MQ=255)                                                                                                                                                                   gCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAg                                                                                                                                                                  <  1:520915/162‑1 (MQ=255)                                                                                                                                                                             ccTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAg                                                                                                                                                                  <  1:777259/152‑1 (MQ=255)                                                                                                                                                                             ccTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCaaaaaaa                                                                                                                             >  1:393973/1‑189 (MQ=255)                                                                                                                                                                                               ccccGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAAt                                             <  1:281973/251‑1 (MQ=255)                                                                                                                                                                                                           cTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTc                                                                                                                                                                                                            >  1:399396/1‑80 (MQ=255)                                                                                                                                                                                                           cTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAGGGTCAACGc                                 <  1:204639/251‑1 (MQ=255)                                                                                                                                                                                                                 tACCGATGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGAc                                                                                <  1:342805/198‑1 (MQ=255)                                                                                                                                                                                                                      aTGGTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGcccc                                                                 <  1:785041/208‑1 (MQ=255)                                                                                                                                                                                                                        ggTTACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAAc                                                           >  1:152350/1‑212 (MQ=255)                                                                                                                                                                                                                          ttACCGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAGGGTCAACGCCGAGCATATTTAATc                  <  1:63453/251‑1 (MQ=255)                                                                                                                                                                                                                             ccGATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAGGGTCAACGCCGAGCATATTTAATCCtt               >  1:44406/1‑251 (MQ=255)                                                                                                                                                                                                                               gATATGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAGGGTCAACGCCGAGCATATTTAATCCTTTa             >  1:839312/1‑251 (MQ=255)                                                                                                                                                                                                                                atatGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCtt                                                                                                                     >  1:189115/1‑146 (MQ=255)                                                                                                                                                                                                                                atatGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAg                                                                                            >  1:639915/1‑171 (MQ=255)                                                                                                                                                                                                                                atatGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAggg                                        >  1:210731/1‑223 (MQ=255)                                                                                                                                                                                                                                  atGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAGGGTCAACGCCGAGCATATTTAATCCTTTATTg          <  1:143671/251‑1 (MQ=255)                                                                                                                                                                                                                                  atGGAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAGGGTCAACGCCGAGCATATTTAATCCTTTATTg          <  1:759024/251‑1 (MQ=255)                                                                                                                                                                                                                                     gAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATAttt                                                                                                                                                                       >  1:861519/1‑91 (MQ=255)                                                                                                                                                                                                                                     gAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAAc                                                           <  1:822251/199‑1 (MQ=255)                                                                                                                                                                                                                                     gAAGACATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAGGGTCAACGCCGAGCATATTTAATCCTTTATTGGt        <  1:360855/250‑1 (MQ=255)                                                                                                                                                                                                                                          cATCCTCACCCGCACTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAGGGTCAACGCCGAGCATATTTAATCCTTTATTGGTTGTAcc  <  1:399805/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              GACATCGCCCGTATGCTCGGCTTGCCTTGCAGCACACCGGAAGAAGGCGTGCGTTCTTACGCACAAGCCTGCTACGATTTAGCTGTGCGTTGTGGCATTAAGATGTCTTTCAAAGAACAAGGCTTGGATGAAAAAGCGTGGATGGATGCCCGTCGTGATGTGGCACTGCTCGCCTTTGAAGACCAATGTTCCCCGGCAAACCCTCGTTTACCGATGGTTACCGATATGGAAGACATCCTCACCCGCGCTTATTTTGGTTATGATCCAAAGGATTATTAATTCACCGTTGTGAGTTCCACAAAAAAGCCTAGAAATATTTTCTAGGCTTTTCTTTTTTCCAGTAAAAGTGCGGTCAAAAAAATTATCCTTTTTTACGACGTTTTAAGGTATCAAGTAATACGGCGACAACGATAATCGCCCCTTTAACAATTTGTTGCCAATAAGGGTCAACGCCGAGCATATTTAATCCTTTATTGGTTGTACC  >  1/2016820‑2017303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑