Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,955,767	T→C	intergenic (+106391/‑44225)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,955,767	0	T	C	100.0%	254.3 / NA	74	intergenic (+106391/‑44225)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/39);  total (35/39)

TCACCGATGCGTTCAGCTAAACCTTGCTCCGCCAGTACTTGTTTCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTTAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGTCATTAATGTCTTTTAA  >  1/1955525‑1956011                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     tCACCGATGCGTTCAGCTAAACCTTGCTCCGCCAGTACTTGTTTCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTaaa                                                                                                                                                                                                                                              >  1:323830/1‑251 (MQ=255)   cccGATGCGTTCAGCTAACCCTTGCTCCGCCAGTACTTGTTTCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAg                                                                                                                                                                                                                                            <  1:269830/250‑1 (MQ=255)           gTTCAGCTAAACCTTGCTCCGCCAGTACTTGTTTCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTa                                                                                                                                                                                                                                    >  1:699561/1‑251 (MQ=255)            ttCAGCTAAACCTTGCTCCGCCAGTACTTGTTTCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTa                                                                                                                                                                                                                                    <  1:170119/250‑1 (MQ=255)            ttCAGCTAAACCTTGCTCCGCCAGTACTTGTTTCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTAt                                                                                                                                                                                                                                   <  1:273016/251‑1 (MQ=255)                                    tACTTGTTTCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAAtc                                                                                                                                                                                                            <  1:275807/250‑1 (MQ=255)                                    tACTTGTTTCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAG‑TTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAAtc                                                                                                                                                                                                            <  1:318306/249‑1 (MQ=255)                                     aCTTGTTTCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAAtct                                                                                                                                                                                                           >  1:249368/1‑250 (MQ=255)                                        tGTTTCTCTGCTGCCAAACGTACCTCTTCGTTTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTa                                                                                                                                                                                                        >  1:413041/1‑251 (MQ=255)                                          tttCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAAc                                                                                                                                                                                                     <  1:789031/251‑1 (MQ=255)                                          tttCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAAc                                                                                                                                                                                                     <  1:15331/251‑1 (MQ=255)                                          tttCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAAc                                                                                                                                                                                                     <  1:571204/251‑1 (MQ=255)                                           ttCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACt                                                                                                                                                                                                    <  1:864335/251‑1 (MQ=255)                                                 gctgcCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAAc                                                                                                                                                                                                                                                   <  1:125436/198‑1 (MQ=255)                                                 gctgcCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTa                                                                                                                                                                                              >  1:356339/1‑251 (MQ=255)                                                    gcCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAg                                                                                                                                                                                                                                            >  1:413024/1‑202 (MQ=255)                                                    gcCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAAc                                                                                                                                                                                                     <  1:489326/241‑1 (MQ=255)                                                      cAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTaaaaa                                                                                                                                                                                          >  1:165286/1‑250 (MQ=255)                                                       aaaCGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGa                                                                                                                                                                                                                                           <  1:413314/200‑1 (MQ=255)                                                           gTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCg                                                                                                                                                                                                                         <  1:120309/214‑1 (MQ=255)                                                                    cG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAg                                                                                                                                                                           <  1:244165/251‑1 (MQ=255)                                                                       ttttttttCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGaa                                                                                                                                                                         <  1:486855/251‑1 (MQ=255)                                                                       ttttttttCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGaa                                                                                                                                                                         <  1:781811/251‑1 (MQ=255)                                                                       ttttttttCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGaa                                                                                                                                                                         <  1:486429/251‑1 (MQ=255)                                                                       ttttttttCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGaa                                                                                                                                                                         <  1:165651/251‑1 (MQ=255)                                                                                           aCGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAg                                                                                                                                                                           >  1:878511/1‑229 (MQ=255)                                                                                            cGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGaaa                                                                                                                                                     >  1:495446/1‑250 (MQ=255)                                                                                              ctctTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGa                                                                                                                                                                          >  1:372165/1‑227 (MQ=255)                                                                                                  tcttcCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGaa                                                                                                                                                                         >  1:341388/1‑224 (MQ=255)                                                                                                     tcCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGc                                                                                                                                           <  1:864046/251‑1 (MQ=255)                                                                                                     tcCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGc                                                                                                                                           <  1:208510/251‑1 (MQ=255)                                                                                                               tttCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAgg                                                                                                                                 <  1:98922/251‑1 (MQ=255)                                                                                                               tttCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAgg                                                                                                                                 <  1:488149/251‑1 (MQ=255)                                                                                                                ttCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTAt                                                                                                                                                                                                                                   <  1:868284/152‑1 (MQ=255)                                                                                                                ttCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAAc                                                                                                                                                                                                     >  1:237054/1‑182 (MQ=255)                                                                                                                    aaaGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATcc                                                                                                                            <  1:609360/251‑1 (MQ=255)                                                                                                                             tgctgcACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCACTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTc                                                                                                                   <  1:447138/251‑1 (MQ=255)                                                                                                                              gctgcACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAgg                                                                                                                                 >  1:50518/1‑236 (MQ=255)                                                                                                                                  cACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAgg                                                                                                                                 >  1:33418/1‑232 (MQ=255)                                                                                                                                      aaaCCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGtt                                                                                                          >  1:171379/1‑251 (MQ=255)                                                                                                                                        aCCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCt                                                                                                                                                                                                                 >  1:209758/1‑146 (MQ=255)                                                                                                                                        aCCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAAtc                                                                                                                                                                                                            <  1:709965/151‑1 (MQ=255)                                                                                                                                         ccTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGttttt                                                                                                       >  1:80488/1‑251 (MQ=255)                                                                                                                                                  gTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTa                                                                                                                                                                                                                                    >  1:368314/1‑117 (MQ=255)                                                                                                                                                      gtgCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAAc                                                                                                                                                                                                     >  1:216335/1‑144 (MQ=255)                                                                                                                                                         ccTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTa                                                                                       >  1:669763/1‑251 (MQ=255)                                                                                                                                                                        tGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCaa                                                                        <  1:741803/251‑1 (MQ=255)                                                                                                                                                                             gCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAAt                                                                   >  1:338245/1‑251 (MQ=255)                                                                                                                                                                              ccTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTa                                                                                                                               >  1:35461/1‑190 (MQ=255)                                                                                                                                                                              ccTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAAt                                                                   <  1:677711/250‑1 (MQ=255)                                                                                                                                                                                tCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAAtt                                                                  >  1:729490/1‑249 (MQ=255)                                                                                                                                                                                    aaGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGctt                                                            <  1:644987/251‑1 (MQ=255)                                                                                                                                                                                                cttcCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGaa                                                                                                                                                                         <  1:142669/130‑1 (MQ=255)                                                                                                                                                                                                            cATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCtt                                    <  1:66791/251‑1 (MQ=255)                                                                                                                                                                                                               tCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAACCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAAt                                                                   <  1:69573/217‑1 (MQ=255)                                                                                                                                                                                                                ccccACATAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATc                                            >  1:537963/1‑239 (MQ=255)                                                                                                                                                                                                                   cacaTAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATc                             <  1:495965/251‑1 (MQ=255)                                                                                                                                                                                                                    acaTAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAAt                              >  1:50355/1‑249 (MQ=255)                                                                                                                                                                                                                     caTAGAACGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCtt                           >  1:145044/1‑251 (MQ=255)                                                                                                                                                                                                                           aCGCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTa                     <  1:653916/251‑1 (MQ=255)                                                                                                                                                                                                                             gCCATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTc                                                       <  1:611434/215‑1 (MQ=255)                                                                                                                                                                                                                              ccATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTaaaaa                                                                                                                                                                                          >  1:133216/1‑83 (MQ=255)                                                                                                                                                                                                                              ccATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGt                  >  1:321205/1‑251 (MQ=255)                                                                                                                                                                                                                              ccATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGt                  >  1:804473/1‑251 (MQ=255)                                                                                                                                                                                                                              ccATAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGt                  >  1:351106/1‑251 (MQ=255)                                                                                                                                                                                                                                 tAGCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGt                                 >  1:567429/1‑233 (MQ=255)                                                                                                                                                                                                                                   gCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCcg                                                                                                                           >  1:789488/1‑141 (MQ=255)                                                                                                                                                                                                                                   gCTAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGTCATTa             >  1:640353/1‑251 (MQ=255)                                                                                                                                                                                                                                     tAACGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGTCATTAAt           <  1:176182/251‑1 (MQ=255)                                                                                                                                                                                                                                      aaCGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGTCATTAATg          <  1:52011/251‑1 (MQ=255)                                                                                                                                                                                                                                       aCGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGTCATTAATGt         <  1:167584/251‑1 (MQ=255)                                                                                                                                                                                                                                       aCGTCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGTCATTAATGt         <  1:66707/251‑1 (MQ=255)                                                                                                                                                                                                                                          tCCATGCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTACACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGCTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTgcagc                                                                          >  1:782191/1‑183 (MQ=255)                                                                                                                                                                                                                                               gCGGTCAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCAAACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGCATCCGCGTCCTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGTCATTAATGTCTTTTaa  >  1:179363/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     TCACCGATGCGTTCAGCTAAACCTTGCTCCGCCAGTACTTGTTTCTCTGCTGCCAAACGTACCTCTTCG‑TTTTTTTTCTCGACGGCAAGACGCTCTTCTTCCGCTTTATTTTCTAAAGCCAGTTGCTGCACCAAACCTAAATCAGTTTGTGCCTGAGTTTGCTCCGTGGCAGCCTCCGAAGGCTTCGTTTCTTCCGCAACCGCATTCCCCACATAGAACGCCATAGCTAACGTCCATGCGGTTAACTTAAAAGATTTCGTATTCATTAATCGCTGTGCCTTAATCTCTAAACTTAGCTAAAAACGACCACACTTTTAGAAAGTGCGGTCAGAAAATGAAACGTTTACGGCTTTGCAGAGGTATCCGCGTCTTTCAGTCCGGTTTTTTCCGCATCAAATTTTACGACATCTGCAGCAATGAATTCTGCTTCTTTCAGAAAGAAATCCGGGGCTTCGTAATCTTTCGGTAAGTCATTAATGTCTTTTAA  >  1/1955525‑1956011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑