Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,868,617	G→A	intergenic (+19241/‑131375)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,868,617	0	G	A	100.0%	206.2 / NA	61	intergenic (+19241/‑131375)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/23);  total (38/23)

CGCTATCTTGATGCAGGGGAAACGGTGGCGGTGAGATTGCGTGTGGAATAATGAATTATCTTAGTCACTTCTGAAAACACGAATCTCCTAGCCATAGCCGTTTGAAATATTTTGGCTCTTTAGTAACTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATGTAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTCCCTATATTCGTGA  >  1/1868375‑1868860                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     cGCTATCTTGATGCAGGGGAAACGGTGGCGGTGAGATTGCGTGTGGAATAATGAATTATCTTAGTCACTTCTGAAAACACGAATCTCCTAGCCATAGCCGTTTGAAATATTTTGGCTCTTTAGTAACTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAg                                                                                                                                                                                                                                              <  1:216190/251‑1 (MQ=255)      tCTTGATGCAGGGGAAACGGTGGCGGTGAGATTGCGTGTGGAATAATGAATTATCTTAGTCACTTCTGAAAACACGAATCTCCTAGCCATAGCCGTTTGAAATATTTTGGCTCTTTAGTAACTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCgg                                                                                                                                                                                                                                         >  1:235329/1‑251 (MQ=255)                                       gCGTGTGGAATAATGAATTATCTTAGTCACTTCTGAAAACACGAATCTCCTAGCCATAGCCGTTTGAAATATTTTGGCTCTTTAGTAACTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGaaa                                                                                                                                                                                                        >  1:641127/1‑251 (MQ=255)                                                          aTCTTAGTCACTTCTGAAAACACGAATCTCCTAGCCATAGCCGCTTGAAATATTCTGGCTCTTTAGTAACTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGCTGTGGTTAGGAAACGTGCCAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGa                                                                                                                                                                                                                               >  1:416175/1‑209 (MQ=255)                                                                              cacGAATCTCCTAGCCATAGCCGTTTGAAATATTTTGGCTCTTTAGTAACTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATatgaa                                                                                                                                                                 <  1:825216/251‑1 (MQ=255)                                                                              cacGAATCTCCTAGCCATAGCCGTTTGAAATATTTTGGCTCTTTAGTAACTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATatgaa                                                                                                                                                                 <  1:241590/251‑1 (MQ=255)                                                                                cGAATCTCCTAGCCATAGCCGTTTGAAATATTTTGGCTCTTTAGTAACTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATatgaatg                                                                                                                                                               <  1:733736/251‑1 (MQ=255)                                                                                                                        ttAGTAACTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTa                                                                                                                       >  1:475210/1‑251 (MQ=255)                                                                                                                              aCTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTaaaaaaa                                                                                                                 >  1:822809/1‑251 (MQ=255)                                                                                                                                           cGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGaaaa                                                                                                    >  1:643375/1‑251 (MQ=255)                                                                                                                                           cGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGaaaa                                                                                                    >  1:635412/1‑251 (MQ=255)                                                                                                                                                     gggTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGc                                                                                                                                                        >  1:488654/1‑189 (MQ=255)                                                                                                                                                     gggTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTaaaaa                                                                                         >  1:528299/1‑251 (MQ=255)                                                                                                                                                     gggTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTaaaaa                                                                                         >  1:395156/1‑251 (MQ=255)                                                                                                                                                       gTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATaaa                                                                                                                                                                                                                                                  <  1:675377/97‑1 (MQ=255)                                                                                                                                                              aCTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAaccgaccg                                                                                >  1:168408/1‑251 (MQ=255)                                                                                                                                                               cTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATaaaaa                                                                                                                                                                                                                                                <  1:599192/91‑1 (MQ=255)                                                                                                                                                               cTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGAt                                                                                                                                                                                                    >  1:117238/1‑135 (MQ=255)                                                                                                                                                                       atatCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTg                                                                         >  1:139579/1‑249 (MQ=255)                                                                                                                                                                        tatCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGCCCCGGGAATGGATGGGGAAAATGCCCCGCACTTTTGCTTAAATTTGATTGATTATGCTAACACGCTCCTCTTAACGCGTTTCCTTTGTTAAAAAAGGTTC‑AATGAAAAACAACTAAAAAACCCAACCGAACTTTGCta                                                                      >  1:72918/1‑251 (MQ=255)                                                                                                                                                                        tatCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATcc                                                                                                                              >  1:152131/1‑196 (MQ=255)                                                                                                                                                                         atCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCtat                                                                     <  1:687604/251‑1 (MQ=255)                                                                                                                                                                         atCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCtat                                                                     <  1:313399/251‑1 (MQ=255)                                                                                                                                                                           cAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATaa                                                                   <  1:845822/251‑1 (MQ=255)                                                                                                                                                                           cAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGCGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATaa                                                                   <  1:340355/251‑1 (MQ=255)                                                                                                                                                                             aTGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTaaaa                                                                                          >  1:30594/1‑226 (MQ=255)                                                                                                                                                                             aTGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATaa                                                                   >  1:564569/1‑249 (MQ=255)                                                                                                                                                                               gTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGAcg                                                                                                                                     <  1:46062/182‑1 (MQ=255)                                                                                                                                                                               gTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATgcg                                                               >  1:87976/1‑251 (MQ=255)                                                                                                                                                                                                cGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGAcgcg                                                                                                                                   >  1:22869/1‑167 (MQ=255)                                                                                                                                                                                                  gTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTg                                                                                                                                                                                                                                >  1:297380/1‑72 (MQ=255)                                                                                                                                                                                                  gTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGACTGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTaa                                            >  1:596926/1‑251 (MQ=255)                                                                                                                                                                                                  gTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATaa                                                                   <  1:369823/228‑1 (MQ=255)                                                                                                                                                                                                  gTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTc                                                  <  1:561506/245‑1 (MQ=255)                                                                                                                                                                                                       ggTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTaaaa                                                                                          >  1:487215/1‑200 (MQ=255)                                                                                                                                                                                                                   tGCTAGCGTAGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAgaga                                 >  1:878858/1‑245 (MQ=255)                                                                                                                                                                                                                            aGTGATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGa                    >  1:215373/1‑249 (MQ=255)                                                                                                                                                                                                                             gTGATTATTAATAGTATGACATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACtt                 >  1:575651/1‑251 (MQ=255)                                                                                                                                                                                                                               gATTATTAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGc                                                                                                                                                        <  1:264638/115‑1 (MQ=255)                                                                                                                                                                                                                                attattAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCa                                                 <  1:670140/216‑1 (MQ=255)                                                                                                                                                                                                                                attattAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTc               >  1:723561/1‑250 (MQ=255)                                                                                                                                                                                                                                attattAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTc               >  1:709259/1‑250 (MQ=255)                                                                                                                                                                                                                                attattAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTc               >  1:128782/1‑250 (MQ=255)                                                                                                                                                                                                                                attattAATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTc               >  1:854554/1‑250 (MQ=255)                                                                                                                                                                                                                                 ttattaATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGa                                                                                                                                       >  1:193098/1‑130 (MQ=255)                                                                                                                                                                                                                                   attaATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACGCGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTccc             <  1:13437/249‑1 (MQ=255)                                                                                                                                                                                                                                   attaATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTCCCta           <  1:639996/251‑1 (MQ=255)                                                                                                                                                                                                                                     taATAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCtat                                                                     <  1:383611/191‑1 (MQ=255)                                                                                                                                                                                                                                       aTAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTCCCTATAtt       >  1:874704/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTAGTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTCCCTATAtt       >  1:777028/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCtat                                                                     >  1:860523/1‑186 (MQ=255)                                                                                                                                                                                                                                          gTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATaa                                                                   >  1:362100/1‑188 (MQ=255)                                                                                                                                                                                                                                          gTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATg                                                                 <  1:901737/190‑1 (MQ=255)                                                                                                                                                                                                                                          gTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAagag                                  <  1:874394/221‑1 (MQ=255)                                                                                                                                                                                                                                          gTATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTCCCTATATTCGt    >  1:342025/1‑251 (MQ=255)                                                                                                                                                                                                                                           tATGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTCCCTATATTCg     <  1:7559/249‑1 (MQ=255)                                                                                                                                                                                                                                            aTGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTc                                                  <  1:601543/203‑1 (MQ=255)                                                                                                                                                                                                                                            aTGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTCCCTATATTCGTGa  >  1:712036/1‑251 (MQ=255)                                                                                                                                                                                                                                            aTGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTCCCTATATTCGTGa  >  1:375810/1‑251 (MQ=255)                                                                                                                                                                                                                                            aTGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTCCCTATATTCGTGa  >  1:256923/1‑251 (MQ=255)                                                                                                                                                                                                                                             tGATATATAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTgg                                                                                                                                                                                                                  <  1:488804/43‑1 (MQ=255)                                                                                                                                                                                                                                               atatatAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATaa                                                                   >  1:333162/1‑183 (MQ=255)                                                                                                                                                                                                                                               atatatAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATaa                                                                   >  1:172139/1‑183 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     CGCTATCTTGATGCAGGGGAAACGGTGGCGGTGAGATTGCGTGTGGAATAATGAATTATCTTAGTCACTTCTGAAAACACGAATCTCCTAGCCATAGCCGTTTGAAATATTTTGGCTCTTTAGTAACTTGGCTCCCCTCGTTTACAGGGGGTAAGATACTTTTGAAATATCAATGTATTACATGTTTCGAACGGTTGTGGTTAGGAAACGTGCTAGCGTAGTGATTATTAATAGTATGATATGTAAAAAAGTGCGGTGGATATTGAAAGTGTTTTTTGGACTCGAGAAATGATTGGGAAAATGACCCGCACTTTTGACTAAATATGAATGATTATGCTGACACGCGTCTCCTGACGCGTATCCTTTGTTAAAAAAAGTTCAAATGAAAAAC‑ACTAAAAAAACCGACCGCACTTTGCTATAATGCGCCCCGTTAATTTCACTTAAAAAATAAGAGACAACCATGACCGACTTTCCCTATATTCGTGA  >  1/1868375‑1868860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑