Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,850,622	G→A	intergenic (+1246/‑149370)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,850,622	0	G	A	100.0%	243.2 / NA	74	intergenic (+1246/‑149370)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/41);  total (33/41)

CCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCGCCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGAATGGCTGGCACAATGGGACATCGGCGACATGGAAGAAACCG  >  1/1850383‑1850865                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    ccATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAAtt                                                                                                                                                                                                                                          <  1:865799/251‑1 (MQ=255)       gCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATgg                                                                                                                                                                                                                                     <  1:881483/250‑1 (MQ=255)            gatTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGa                                                                                                                                                                                                                                >  1:875426/1‑250 (MQ=255)                ggTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACgggg                                                                                                                                                                                                                           >  1:77559/1‑251 (MQ=255)                   tgattgatGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCtt                                                                                                                                                                                                                        <  1:220733/251‑1 (MQ=255)                    gattgatGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTg                                                                                                                                                                                                                       >  1:726159/1‑251 (MQ=255)                       tgatGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGgtg                                                                                                                                                                                                                    <  1:119385/251‑1 (MQ=255)                       tgatGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGgtg                                                                                                                                                                                                                    <  1:122959/251‑1 (MQ=255)                        gatGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGgtgt                                                                                                                                                                                                                   >  1:831207/1‑251 (MQ=255)                           gCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGtgtg                                                                                                                                                                                                                  >  1:510709/1‑249 (MQ=255)                            ccGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTc                                                                                                                                                                                                               <  1:30637/251‑1 (MQ=255)                            ccGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTc                                                                                                                                                                                                               <  1:352143/251‑1 (MQ=255)                            ccGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTc                                                                                                                                                                                                               <  1:481424/251‑1 (MQ=255)                            ccGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTc                                                                                                                                                                                                               <  1:236539/251‑1 (MQ=255)                                  cGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTgg                                                                                                                                                                                                          <  1:610632/250‑1 (MQ=255)                                      aCGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGt                                                                                                                                                                                                     <  1:363458/251‑1 (MQ=255)                                              aaCTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCa                                                                                                                                                                                             >  1:294892/1‑251 (MQ=255)                                                cTCAATTCACGCACAAAATTGGTGGCGTTAAATTTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCAc                                                                                                                                                                                            <  1:725102/251‑1 (MQ=255)                                                  cAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTgg                                                                                                                                                                                         >  1:545886/1‑251 (MQ=255)                                                    aTTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCGACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGAt                                                                                                                                                                                       <  1:90523/251‑1 (MQ=255)                                                         cGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACt                                                                                                                                                                                  <  1:157504/251‑1 (MQ=255)                                                           cacaAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTg                                                                                                                                                                                 <  1:818354/250‑1 (MQ=255)                                                           cacaAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGc                                                                                                                                                                                <  1:216034/251‑1 (MQ=255)                                                           cacaAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACATTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGTTTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTg                                                                                                                                                                                 >  1:540930/1‑250 (MQ=255)                                                               aaaTTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAAc                                                                                                                                                                             >  1:770135/1‑250 (MQ=255)                                                                         cGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATAc                                                                                                                                                                  <  1:547803/251‑1 (MQ=255)                                                                                          gAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGa                                                                                                                                                     <  1:535/248‑1 (MQ=255)                                                                                            aCGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAAt                                                                                                                                                >  1:723780/1‑251 (MQ=255)                                                                                               ttACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATaa                                                                                                                                              <  1:372162/250‑1 (MQ=255)                                                                                                 aCCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGt                                                                                                                                           <  1:787590/251‑1 (MQ=255)                                                                                                                 cGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGATCTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGt                                                                                                                                                                                                     <  1:195739/177‑1 (MQ=255)                                                                                                                 cGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATAc                                                                                                                                                                  >  1:429753/1‑212 (MQ=255)                                                                                                                 cGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATAc                                                                                                                                                                  <  1:469108/212‑1 (MQ=255)                                                                                                                 cGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGt                                                                                                                                           <  1:74989/235‑1 (MQ=255)                                                                                                                 cGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAaacaac                                                                                                                                >  1:407582/1‑246 (MQ=255)                                                                                                                   gTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGgtg                                                                                                                                                                                                                    >  1:748079/1‑160 (MQ=255)                                                                                                                              gATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAAt                                                                                                                                                >  1:136599/1‑217 (MQ=255)                                                                                                                                    gcgcATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCtt                                                                                                        >  1:398168/1‑251 (MQ=255)                                                                                                                                       cATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTAt                                                                                                      >  1:873576/1‑250 (MQ=255)                                                                                                                                           cGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATAc                                                                                                                                                                  >  1:289599/1‑186 (MQ=255)                                                                                                                                           cGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATAc                                                                                                                                                                  <  1:697740/186‑1 (MQ=255)                                                                                                                                           cGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAg                                                                                                                                            <  1:85544/208‑1 (MQ=255)                                                                                                                                           cGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGttt                                                                                                 >  1:264778/1‑251 (MQ=255)                                                                                                                                                cAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAg                                                                                                                                            >  1:631360/1‑203 (MQ=255)                                                                                                                                                cAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAg                                                                                            >  1:736972/1‑251 (MQ=255)                                                                                                                                                          gCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAg                                                                                                                                                                                                      <  1:589015/135‑1 (MQ=255)                                                                                                                                                          gCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGc                                                                                                                                                                                >  1:357999/1‑157 (MQ=255)                                                                                                                                                          gCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGc                                                                                   >  1:645426/1‑250 (MQ=255)                                                                                                                                                               gATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGc                                                                                           >  1:267485/1‑237 (MQ=255)                                                                                                                                                               gATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGTCACGCCGAata                                                                             >  1:462256/1‑251 (MQ=255)                                                                                                                                                               gATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAata                                                                             >  1:271347/1‑251 (MQ=255)                                                                                                                                                                aTCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAg                                                                                                                                                                                                      >  1:455495/1‑129 (MQ=255)                                                                                                                                                                aTCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAatat                                                                            >  1:10389/1‑251 (MQ=255)                                                                                                                                                                  cttgcttgACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTg                                                                          <  1:862062/251‑1 (MQ=255)                                                                                                                                                                   ttgcttgACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTgc                                                                         <  1:764957/251‑1 (MQ=255)                                                                                                                                                                              cgcgCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCg                                                              <  1:243355/251‑1 (MQ=255)                                                                                                                                                                                  cAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGt                                                                                                                                                                                                     >  1:389001/1‑112 (MQ=255)                                                                                                                                                                                    ggCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCa                                                        >  1:506088/1‑251 (MQ=255)                                                                                                                                                                                        aTTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTCCCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAAcg                                                     <  1:883825/250‑1 (MQ=255)                                                                                                                                                                                                caTAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAg                                                                                                                                                                                                      >  1:148613/1‑97 (MQ=255)                                                                                                                                                                                                  tAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGaa                                          <  1:797481/251‑1 (MQ=255)                                                                                                                                                                                                  tAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGaa                                          <  1:529533/251‑1 (MQ=255)                                                                                                                                                                                                  tAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGaa                                          <  1:7569/251‑1 (MQ=255)                                                                                                                                                                                                  tAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGaa                                          <  1:288244/251‑1 (MQ=255)                                                                                                                                                                                                       aTCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGAAtggct                                     <  1:32654/251‑1 (MQ=255)                                                                                                                                                                                                           aTTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGAATGGCTGGca                                 >  1:888468/1‑251 (MQ=255)                                                                                                                                                                                                                gCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCAAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGAATGGCTGGCACAATg                            >  1:7505/1‑251 (MQ=255)                                                                                                                                                                                                                    aaCTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGAATGGCTGGCACAATgg                           <  1:794375/248‑1 (MQ=255)                                                                                                                                                                                                                     aCTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGaa                                          <  1:122252/232‑1 (MQ=255)                                                                                                                                                                                                                                aTTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTAGAAGCCGGCACGCCGAATATTGCAGCCGTTATCGGTTTCAACGCGGTGTTGGAATGGCTGGCACAATGGGACATCGGCGACAt             <  1:858634/250‑1 (MQ=255)                                                                                                                                                                                                                                      tCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGAATGGCTGGCACAATGGGACATCGGCGACATGgaagaa      <  1:867985/251‑1 (MQ=255)                                                                                                                                                                                                                                        gcgTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCt                                                                                                                       <  1:786380/136‑1 (MQ=255)                                                                                                                                                                                                                                         cgTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGAATGGCTGGCACAATGGGACATCGGCGACATGGAAGAAAcc   <  1:255834/251‑1 (MQ=255)                                                                                                                                                                                                                                           ttttCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGAATGGCTGGCACAATGGGACATCGGCGACATGGAAGAAAccg  >  1:657278/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    CCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCGCCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGCCGCCGTTATCGGTTTCAACGCGGTGTTGGAATGGCTGGCACAATGGGACATCGGCGACATGGAAGAAACCG  >  1/1850383‑1850865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑