Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	354,711	A→G	intergenic (‑7889/+57761)	unknown ← / ← unknown	tRNA‑Ser‑GCT/tRNA‑Ala‑TGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	354,711	0	A	G	100.0%	290.8 / NA	83	intergenic (‑7889/+57761)	unknown/unknown	tRNA‑Ser‑GCT/tRNA‑Ala‑TGC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/46);  total (37/46)

CTTTTCATCTTTTGTAAACTTTGAATTGCTTTTTGACGTTCTTCTCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGATCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAAGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTAAAGCATTCGCCATAATTTGGGCTGTTGCAATTTCATCATAAAATTTAACATC  >  1/354469‑354938                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    cTTTTCATCTTTTGTAAACTTTGAATTGCTTTTTGACGTTCTTCTCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTc                                                                                                                                                                                                                             <  1:346325/251‑1 (MQ=255)       aTCTTTTGTAAACTTTGAATTGCTTTTTGACGTTCTTCTCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATac                                                                                                                                                                                                                       <  1:140002/251‑1 (MQ=255)       aTCTTTTGTAAACTTTGAATTGCTTTTTGACGTTCTTCTCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATac                                                                                                                                                                                                                       <  1:273570/251‑1 (MQ=255)       aTCTTTTGTAAACTTTGAATTGCTTTTTGACGTTCTTCTCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATac                                                                                                                                                                                                                       <  1:52087/251‑1 (MQ=255)                       gAATTGCTTTTTGACGTTCTTCTCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCa                                                                                                                                                                                                                            <  1:836787/230‑1 (MQ=255)                            gCTTTTTGACGTTCTTCTCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATac                                                                                                                                                                                                                       >  1:494827/1‑230 (MQ=255)                            gCTTTTTGACGTTCTTCTCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATac                                                                                                                                                                                                                       >  1:519436/1‑230 (MQ=255)                                    aCGTTCTTCTCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCg                                                                                                                                                                                           <  1:269715/250‑1 (MQ=255)                                          ttctCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTAc                                                                                                                                                                                    <  1:550831/251‑1 (MQ=255)                                           tctcCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCTAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATac                                                                                                                                                                                                                       >  1:230482/1‑215 (MQ=255)                                              ccACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCata                                                                                                                                                                                >  1:322995/1‑251 (MQ=255)                                                aCTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCatat                                                                                                                                                                                                                         >  1:33904/1‑208 (MQ=255)                                                aCTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCAtata                                                                                                                                                                              >  1:513875/1‑251 (MQ=255)                                                aCTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCAtata                                                                                                                                                                              >  1:280191/1‑251 (MQ=255)                                                   aaGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAAt                                                                                                                                                                           <  1:853546/251‑1 (MQ=255)                                                     gTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAg                                                                                                                                                                         >  1:357802/1‑251 (MQ=255)                                                       tCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAgtg                                                                                                                                                                       <  1:561502/251‑1 (MQ=255)                                                             cAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATac                                                                                                                                                                                                                       <  1:336398/197‑1 (MQ=255)                                                             cAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAAt                                                                                                                                                                  >  1:148319/1‑250 (MQ=255)                                                                ttACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCat                                                                                                                                                                                                                           >  1:206504/1‑190 (MQ=255)                                                                ttACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTAc                                                                                                                                                                                    >  1:503460/1‑229 (MQ=255)                                                                      tCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGTTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAAc                                                                                                                                                        <  1:431566/251‑1 (MQ=255)                                                                           gTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATacc                                                                                                                                                                                                                      >  1:105194/1‑184 (MQ=255)                                                                           gTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATac                                                                                                                                                                                                                       <  1:884111/183‑1 (MQ=255)                                                                           gTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACaataa                                                                                                                                                   >  1:249939/1‑251 (MQ=255)                                                                           gTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACaataa                                                                                                                                                   >  1:751598/1‑251 (MQ=255)                                                                             ttttAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAataata                                                                                                                                                 <  1:114456/251‑1 (MQ=255)                                                                               ttAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATaatatc                                                                                                                                               <  1:82873/251‑1 (MQ=255)                                                                               ttAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATaatatc                                                                                                                                               <  1:514369/251‑1 (MQ=255)                                                                                tAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATaatatca                                                                                                                                              <  1:811765/251‑1 (MQ=255)                                                                                            tatttGTGCTCATTAATACATGCGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAAt                                                                                                                                                                           >  1:521177/1‑210 (MQ=255)                                                                                             atttGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCAtata                                                                                                                                                                                                                        >  1:700546/1‑164 (MQ=255)                                                                                             atttGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACtt                                                                                                                                 <  1:898561/251‑1 (MQ=255)                                                                                                 gtgCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTg                                                                                                                             >  1:12911/1‑251 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTc                                                                                                                                                                                                                             >  1:57313/1‑150 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATac                                                                                                                                                                                                                       >  1:652530/1‑156 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTc                                                                                                                                                                                         <  1:863458/186‑1 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAAt                                                                                                                                                                           <  1:672017/200‑1 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAg                                                                                                                                                                         >  1:538654/1‑202 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATaatatcaatat                                                                                                                                          <  1:326145/233‑1 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATaatatc                                                                                                                                               <  1:148958/228‑1 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATaatat                                                                                                                                                <  1:305527/227‑1 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACtt                                                                                                                                 <  1:897186/242‑1 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCaa                                                                                                                        >  1:499897/1‑251 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCaa                                                                                                                        >  1:284912/1‑251 (MQ=255)                                                                                                      cATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCaa                                                                                                                        >  1:90405/1‑251 (MQ=255)                                                                                                        ttAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATaccac                                                                                                                                                                                                                    >  1:637732/1‑157 (MQ=255)                                                                                                        ttAATACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATaatatcaatat                                                                                                                                          <  1:332607/231‑1 (MQ=255)                                                                                                          aaTACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAAt                                                                                                                                                                           >  1:882805/1‑196 (MQ=255)                                                                                                            tACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTAc                                                                                                                                                                                    <  1:203256/185‑1 (MQ=255)                                                                                                            tACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTa                                                                                                                  <  1:765079/251‑1 (MQ=255)                                                                                                            tACATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTa                                                                                                                  <  1:355041/251‑1 (MQ=255)                                                                                                              cATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATac                                                                                                                                                                                                                       <  1:410551/148‑1 (MQ=255)                                                                                                              cATGTGGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACtt                                                                                                                                 >  1:488105/1‑234 (MQ=255)                                                                                                                  tgGAGTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTc                                                                                                             <  1:666020/250‑1 (MQ=255)                                                                                                                      gTTTTTCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATaatat                                                                                                                                                >  1:169549/1‑211 (MQ=255)                                                                                                                        ttttCGGATTATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCTATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCAc                                                                                                      <  1:451869/251‑1 (MQ=255)                                                                                                                                ttATGGAAGCGGTCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGatat                                                                                                                                                                                                                               >  1:898117/1‑122 (MQ=255)                                                                                                                                         cggtCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAg                                                                                                                                                                         >  1:837383/4‑167 (MQ=255)                                                                                                                                         cggtCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTAATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGaaa                                                                                       <  1:203615/246‑1 (MQ=255)                                                                                                                                                           aTTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATGAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAg                                                                                  <  1:750218/236‑1 (MQ=255)                                                                                                                                                                 cGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACAcc                                                                 >  1:620500/1‑247 (MQ=255)                                                                                                                                                                                       ggAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATaccac                                                                                                                                                                                                                    <  1:84267/78‑1 (MQ=255)                                                                                                                                                                                        gAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAg                                                                                                                                                                         <  1:641917/120‑1 (MQ=255)                                                                                                                                                                                        gAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATaatat                                                                                                                                                <  1:389068/145‑1 (MQ=255)                                                                                                                                                                                        gAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTa                                                      <  1:511342/235‑1 (MQ=255)                                                                                                                                                                                        gAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATACTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGAt                                                                                >  1:25203/1‑209 (MQ=255)                                                                                                                                                                                               cccAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTAAAGCATTCGCCATAATTTGGGc                                <  1:397481/250‑1 (MQ=255)                                                                                                                                                                                                   aGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACaa                                                                                                                                                      <  1:559475/128‑1 (MQ=255)                                                                                                                                                                                                     cTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAacac                                                                  <  1:320996/210‑1 (MQ=255)                                                                                                                                                                                                        ccATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTAAAGCATTCGCCATAATTTGGGCTGTTGCAAtt                      <  1:699975/251‑1 (MQ=255)                                                                                                                                                                                                        ccATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTAAAGCATTCGCCATAATTTGGGCTGTTGCAAtt                      <  1:697763/251‑1 (MQ=255)                                                                                                                                                                                                        ccATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTAAAGCATTCGCCATAATTTGGGCTGTTGCAAtt                      <  1:37517/251‑1 (MQ=255)                                                                                                                                                                                                            cacaAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTAAAGCATTCGCCATAATTTGGGCTGTTGCAATTtcat                  >  1:96143/1‑251 (MQ=255)                                                                                                                                                                                                                    cAGGTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTAc                                                                                                                                                                                    <  1:582652/81‑1 (MQ=255)                                                                                                                                                                                                                      ggTTCACCATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTAAAGCATTCGCCATAATTTGGGCTGTTGCAAtt                      >  1:229692/1‑237 (MQ=255)                                                                                                                                                                                                                             cATTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATaa                                                                              <  1:269377/174‑1 (MQ=255)                                                                                                                                                                                                                              aTTCGGGCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTAAAGTATTCGCCATAATTTGGGCTGTTGCAATTTCATCATAAAATTTAACATc  >  1:619082/1‑249 (MQ=255)                                                                                                                                                                                                                                   ggCTTTTAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAAtt                                                                                                                                                                                                      <  1:446463/48‑1 (MQ=255)                                                                                                                                                                                                                                       tttAAATTCTAAGGGATATTCATATTCCACCGGCAATTTCAATTCACGCAACTCGTCATTTCCATATAAATAGTGTGAATCGCCGGTAACAATaatatcaatat                                                                                                                                          <  1:473703/104‑1 (MQ=255)                                                                                                                                                                                                                                       tttAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCatat                                                                                                                                                                               >  1:865207/1‑67 (MQ=255)                                                                                                                                                                                                                                       tttAAATTCTAAGGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAAt                                                                                                                                                                           >  1:853856/1‑71 (MQ=255)                                                                                                                                                                                                                                            aTTCTAAGGGATATTCATATACCACCGGCGATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTAAAGCATTCGCCATAAt                                      >  1:781796/1‑199 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    CTTTTCATCTTTTGTAAACTTTGAATTGCTTTTTGACGTTCTTCTCCACTAAGTTCCTGCCAATTACCGTCACTGTTTTTAACTTTTAATTTATTTGTGCTCATTAATACATGTGGAGTTTTTCGGATTATGGAAGCGATCCCATCTTTGCTGAATTTAACGCCTAAATCGCCCACTACGGCGGAATAAGCCCAAGCTTCCATCACAAAAACAGGTTCACCATTCGGGCTTTTAAATTCTAAAGGATATTCATATACCACCGGCAATTTCAATTCACGCAACTCGTCATTACCATATAAATAGTGTGAATCGCCGGTAACAATAATATCAATATCATTTACTTTTTGCGCAATTTCTATGTTCTTTTCACTGCCCGCATGGGAAAGTAAGATAATTTTGTTAACACCCTGTGCTTTTAAAGCATTCGCCATAATTTGGGCTGTTGCAATTTCATCATAAAATTTAACATC  >  1/354469‑354938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑