Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,614,088	A→G	intergenic (‑12212/+7091)	unknown ← / ← unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,614,088	0	A	G	100.0%	185.2 / NA	53	intergenic (‑12212/+7091)	unknown/unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/21);  total (32/21)

ATCGCCCGTCGGGTTCATACCAATAACCAAAATCTTCCAGTTTGCGACGCTCTTTGCCTGCAACCAGCCAATGGGCGATTTCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTATGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTATTCTTTGGTTCTTCCC  >  1/1613844‑1614332                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     aTCGCCCGTCGGGTTCATACCAATAACCAAAATCTTCCAGTTTGCGACGCTCTTTGCCTGCAACCAGCCAATGGGCGATTGCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTc                                                                                                                                                                                                                                                 <  1:438255/250‑1 (MQ=255)    gCCCGTCGGGTTCATACCAATAACCAAAATCTTCCAGTTTGCGACGCTCTTTGCCTGCAACCAGCCAATGGGCGATTTCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCata                                                                                                                                                                                                                                             >  1:677959/1‑251 (MQ=255)             gTTCATACCAATAACCAAAATCTTCCAGTTTGCGACGCTCTTTGCCTGCAACCAGCCAATGGGCGATTTCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTcc                                                                                                                                                                                                                                     >  1:733043/1‑250 (MQ=255)                    ccAATAACCAAAATCTTCCAGTTTGCGACGCTCTTTGCCTGCAACCAGCCAATGGGCGATTTCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAAc                                                                                                                                                                                                                             <  1:300060/251‑1 (MQ=255)                           ccAAAATCTTCCAGTTTGCGACGCTCTTTGCCTGCAACCAGCCAATGGGCGATTTCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGcc                                                                                                                                                                                                                      <  1:682486/251‑1 (MQ=255)                                  cTTCCAGTTTGCGACGCTCTTTGCCTGCAACCAGCCAATGGGCGATTTCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAAt                                                                                                                                                                                                               <  1:454574/251‑1 (MQ=255)                                                  ctctTTGCCTGCAACCAGCCAATGGGCGATTTCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAAcc                                                                                                                                                                                                          >  1:214984/1‑240 (MQ=255)                                                   tctTTGCCTGCAACCAGCCAATGGGCGATTTCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAg                                                                                                                                                                                              <  1:500247/251‑1 (MQ=255)                                                                   gCCAATGGGCGATTTCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCAAGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAAt                                                                                                                                                                                   <  1:265686/246‑1 (MQ=255)                                                                                   aTGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACaa                                                                                                                                                                                                >  1:568085/1‑217 (MQ=255)                                                                                    tGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTc                                                                                                                                                                          <  1:182582/238‑1 (MQ=255)                                                                                               gctgTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTgtgt                                                                                                                                                                                                                                                   <  1:822416/154‑1 (MQ=255)                                                                                               gctgTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAAc                                                                                                                                                                                                                             >  1:746480/1‑176 (MQ=255)                                                                                               gctgTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAg                                                                                                                                                                            >  1:134047/1‑225 (MQ=255)                                                                                               gctgTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCct                                                                                                                                                   >  1:128820/1‑250 (MQ=255)                                                                                               gctgTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCct                                                                                                                                                   >  1:561953/1‑250 (MQ=255)                                                                                                                cAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAAt                                                                                                                                                                                   >  1:314469/1‑201 (MQ=255)                                                                                                                 aaaaTAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTg                                                                                                                                                                                       >  1:552851/1‑196 (MQ=255)                                                                                                                  aaaTAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGa                                                                                                                               <  1:763900/251‑1 (MQ=255)                                                                                                                    atagatagCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTaaa                                                                                                                                  >  1:491300/1‑246 (MQ=255)                                                                                                                     tagatagCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAg                                                                                                                                          >  1:136681/1‑237 (MQ=255)                                                                                                                           gCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAat                                                                                                                       >  1:876733/1‑250 (MQ=255)                                                                                                                           gCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAat                                                                                                                       >  1:264455/1‑250 (MQ=255)                                                                                                                               tGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAAcc                                                                                                                                                                                                          <  1:616362/163‑1 (MQ=255)                                                                                                                                    ggACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCaaaa                                                                                                                                                        >  1:334570/1‑208 (MQ=255)                                                                                                                                        aaTCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTcc                                                                                                         <  1:40036/251‑1 (MQ=255)                                                                                                                                             tCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAAcc                                                                                                                                                                                                          >  1:226688/1‑149 (MQ=255)                                                                                                                                               aTTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCAc                                                                                                  <  1:599836/251‑1 (MQ=255)                                                                                                                                                   gCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTtg                                                                                              >  1:143825/1‑251 (MQ=255)                                                                                                                                                              cAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGc                                                                                   <  1:100555/251‑1 (MQ=255)                                                                                                                                                                      cATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGca                                                                           <  1:498075/251‑1 (MQ=255)                                                                                                                                                                       aTCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGc                                                                                                                                         <  1:206060/188‑1 (MQ=255)                                                                                                                                                                        tCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCaaaa                                                                                                                                                        >  1:82340/1‑172 (MQ=255)                                                                                                                                                                              cccTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGcc                                                                                                                                                    >  1:243864/1‑170 (MQ=255)                                                                                                                                                                              cccTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAg                                                                                                                                <  1:860945/190‑1 (MQ=255)                                                                                                                                                                                   gACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCAttt                                                              >  1:250128/1‑251 (MQ=255)                                                                                                                                                                                            ggTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCtt                                                     >  1:417858/1‑251 (MQ=255)                                                                                                                                                                                               gttgtATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCttttt                                                                                                                                             >  1:368648/1‑160 (MQ=255)                                                                                                                                                                                                ttgtATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTg                                                                    >  1:20631/1‑232 (MQ=255)                                                                                                                                                                                                    aTTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCttt                                              >  1:199576/1‑250 (MQ=255)                                                                                                                                                                                                     ttCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTcc                                                                                                         <  1:517838/190‑1 (MQ=255)                                                                                                                                                                                                         tGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGgctg                                        <  1:131440/251‑1 (MQ=255)                                                                                                                                                                                                            tCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGgctg                                        >  1:712778/1‑248 (MQ=255)                                                                                                                                                                                                            tCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGgctg                                        >  1:331396/1‑248 (MQ=255)                                                                                                                                                                                                               aaaCATTGATTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAgg                                  <  1:879311/251‑1 (MQ=255)                                                                                                                                                                                                                       aTTAAAAATATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCtt                          <  1:137385/251‑1 (MQ=255)                                                                                                                                                                                                                           aaaaTATGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAg                                                                                                                                                                            >  1:796635/1‑101 (MQ=255)                                                                                                                                                                                                                                atGGATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTAt                 <  1:635380/251‑1 (MQ=255)                                                                                                                                                                                                                                  ggATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTAtt                >  1:212889/1‑250 (MQ=255)                                                                                                                                                                                                                                   gATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTAtt                >  1:725056/1‑249 (MQ=255)                                                                                                                                                                                                                                   gATTAAATCTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTAtt                >  1:365382/1‑249 (MQ=255)                                                                                                                                                                                                                                           cTTCGATTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTATTCTTTGGtt        >  1:565891/1‑249 (MQ=255)                                                                                                                                                                                                                                                aTTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGaa                                                                                                                        >  1:744263/1‑132 (MQ=255)                                                                                                                                                                                                                                                aTTTTGTGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTATTCTTTGGTTCTTccc  <  1:629362/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ATCGCCCGTCGGGTTCATACCAATAACCAAAATCTTCCAGTTTGCGACGCTCTTTGCCTGCAACCAGCCAATGGGCGATTTCATGTAATCCACTGCTGTAGAATCCGCGGGCAAAATAGATAGCGTTGTAAGGACAATCTTCATTTGCCGGCACGTACAAAGGCTCATCGCCACCCTTGACTAATTTGGTGTTGTATTCCTGTTCAAAACATTGATTAAAAATATGGATTAAATCTTCGATTTTATGTTCCATATCGACTCCCGAAAAACTGACGCCTAAAAATAAACCCGCTTAACAAAGCGGGTTGTAATCATAAAGTCTTTGCAACTAGCTCAAAAAGCCTCTTTTTTAGCTATTAAAAGATTTGGAATCATCATTTTTTTCCACCGCACTTTGTGTTTCCACGCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTATTCTTTGGTTCTTCCC  >  1/1613844‑1614332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑