Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,597,756	C→G	intergenic (+265234/+4044)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,597,756	0	C	G	100.0%	262.3 / NA	76	intergenic (+265234/+4044)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (38/38);  total (38/38)

GGATAATAAAGCTAACATTAGAACAAATTCTCCAAAGCCTGAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTTTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATCGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACGTCAAATGTTTGATTTAACTTCAAATAAAACTGCATTTCTTT  >  1/1597526‑1597998                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ggATAATAAAGCTAACATTAGAACAAATTCTCCAAAGCCTGAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCTGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGc                                                                                                                                                                                                                                       <  1:148820/244‑1 (MQ=255)                cATTAGAACAAATTCTCCAAAGCCTGAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTgcg                                                                                                                                                                                                                  >  1:412877/1‑250 (MQ=255)                cATTAGAACAAATTCTCCAAAGCCTGAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTgcg                                                                                                                                                                                                                  >  1:480052/1‑250 (MQ=255)                     gAACAAATTCTCCAAAGCCTGAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGttt                                                                                                                                                                                                                      >  1:343717/1‑241 (MQ=255)                         aaaTTCTCCAAAGCCTGAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGcc                                                                                                                                                                                                        <  1:620299/251‑1 (MQ=255)                                  aaaGCCTGAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAAtt                                                                                                                                                                                               <  1:275037/251‑1 (MQ=255)                                     gCCTGAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGa                                                                                                                                                                                            >  1:606832/1‑251 (MQ=255)                                     gCCTGAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGa                                                                                                                                                                                            >  1:652857/1‑251 (MQ=255)                                         gAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAATCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTg                                                                                                                                                                                        >  1:443341/1‑251 (MQ=255)                                              aaCACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAAt                                                                                                                                                                                    >  1:280202/1‑250 (MQ=255)                                                     aaTCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCaaa                                                                                                                                                                            >  1:707394/1‑251 (MQ=255)                                                            tttATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAatat                                                                                                                                                                      <  1:132608/250‑1 (MQ=255)                                                               aTCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGc                                                                                                                                                                                                                                       >  1:525603/1‑182 (MQ=255)                                                                tCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGGCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTcc                                                                                                                                                                 <  1:437135/251‑1 (MQ=255)                                                                            ccGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATaaaa                                                                                                                                                     <  1:700626/251‑1 (MQ=255)                                                                              gTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAaca                                                                                                                                                   >  1:257440/1‑251 (MQ=255)                                                                               ttttGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAacac                                                                                                                                                  <  1:846134/251‑1 (MQ=255)                                                                               ttttGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAacac                                                                                                                                                  <  1:490875/251‑1 (MQ=255)                                                                                                     cACGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAAc                                                                                                                            <  1:58330/251‑1 (MQ=255)                                                                                                       cGTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACAt                                                                                                                          <  1:188945/251‑1 (MQ=255)                                                                                                        gTAAGAAACAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCa                                                                                                                                          <  1:524791/234‑1 (MQ=255)                                                                                                             aaaCAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCttt                                                                                                                    <  1:784013/251‑1 (MQ=255)                                                                                                                cAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAAt                                                                                                                 >  1:690891/1‑251 (MQ=255)                                                                                                                cAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAAt                                                                                                                 >  1:823650/1‑251 (MQ=255)                                                                                                                cAATAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAAt                                                                                                                 >  1:628489/1‑251 (MQ=255)                                                                                                                  aTAAAACACACCGCCGAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCg                                                                                                               >  1:516103/1‑251 (MQ=255)                                                                                                                            ccgccgAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATc                                                                                                                >  1:409290/1‑240 (MQ=255)                                                                                                                            ccgccgAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTg                                                                                                     <  1:395261/251‑1 (MQ=255)                                                                                                                              gccgAAATGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTaa                                                                                                                                                                                     <  1:151876/169‑1 (MQ=255)                                                                                                                                     tGGGTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGtt                                                                                             >  1:734994/1‑250 (MQ=255)                                                                                                                                      gggTGTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCttt                                                                                                                    <  1:330245/226‑1 (MQ=255)                                                                                                                                       ggtgtcatagtcatagTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATc                                                                                                                <  1:71763/225‑1 (MQ=255)                                                                                                                                        gtgtcatagtcatagTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATc                                                                                                                >  1:701602/4‑228 (MQ=255)                                                                                                                                              tagtcatagtTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAg                                                                                        >  1:142545/1‑246 (MQ=255)                                                                                                                                                gtcatagtTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAacac                                                                                                                                                  >  1:724866/1‑186 (MQ=255)                                                                                                                                                gtcatagtTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACcg                                                                                                                                  <  1:251442/202‑1 (MQ=255)                                                                                                                                                gtcatagtTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAgt                                                                                 >  1:351404/1‑251 (MQ=255)                                                                                                                                                  catagtTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAgtgg                                                                               <  1:79710/251‑1 (MQ=255)                                                                                                                                                  catagtTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAgtgg                                                                               >  1:399457/1‑251 (MQ=255)                                                                                                                                                        ttGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGc                                                                         <  1:111368/251‑1 (MQ=255)                                                                                                                                                           cGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCtg                                                                       <  1:229612/250‑1 (MQ=255)                                                                                                                                                                cGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTaa                                                                 <  1:663346/251‑1 (MQ=255)                                                                                                                                                                cGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCATAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTaa                                                                 <  1:255441/251‑1 (MQ=255)                                                                                                                                                                   tgttgtAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCCCGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGc                                                              <  1:95477/251‑1 (MQ=255)                                                                                                                                                                    gttgtAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGaa                                                                                                                                      >  1:3186/1‑178 (MQ=255)                                                                                                                                                                    gttgtAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTaa                                                                 <  1:174093/247‑1 (MQ=255)                                                                                                                                                                    gttgtAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACg                                                               >  1:73376/1‑249 (MQ=255)                                                                                                                                                                    gttgtAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACg                                                               >  1:217/1‑249 (MQ=255)                                                                                                                                                                       gtAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGt                                                                                                                                                                                                                           >  1:601932/1‑90 (MQ=255)                                                                                                                                                                       gtAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTaa                                                                 <  1:277821/244‑1 (MQ=255)                                                                                                                                                                       gtAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGa                                                          >  1:438305/1‑251 (MQ=255)                                                                                                                                                                       gtAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGa                                                          >  1:639225/1‑251 (MQ=255)                                                                                                                                                                            tAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAgtgg                                                                               <  1:197549/225‑1 (MQ=255)                                                                                                                                                                             aaCGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAac                                                    <  1:132023/251‑1 (MQ=255)                                                                                                                                                                                 gTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGt                                                                                     <  1:581839/214‑1 (MQ=255)                                                                                                                                                                                 gTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGc                                                                         >  1:602963/1‑226 (MQ=255)                                                                                                                                                                                      aTGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAacac                                                                                                                                                  <  1:678840/148‑1 (MQ=255)                                                                                                                                                                                      aTGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAg                                                                                        <  1:416525/206‑1 (MQ=255)                                                                                                                                                                                      aTGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTaa                                                                 <  1:531979/229‑1 (MQ=255)                                                                                                                                                                                      aTGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACg                                           >  1:883640/1‑251 (MQ=255)                                                                                                                                                                                             cGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACGTCAAATg                                    <  1:495941/251‑1 (MQ=255)                                                                                                                                                                                             cGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACGTCAAATg                                    <  1:775891/251‑1 (MQ=255)                                                                                                                                                                                                   aaataaatATTGCCAGTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAggg                                                                                      >  1:171530/1‑195 (MQ=255)                                                                                                                                                                                                                  gTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGc                                                              <  1:774893/204‑1 (MQ=255)                                                                                                                                                                                                                  gTCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACGTCAAATGTTTGATTTAACTTCAAATaaa               >  1:741922/1‑251 (MQ=255)                                                                                                                                                                                                                   tCGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAAc                                                                                                                                    >  1:340084/1‑133 (MQ=255)                                                                                                                                                                                                                    cGTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACGTCAAATGTTTGATTTAACTTCAAATAAAAc             <  1:151892/251‑1 (MQ=255)                                                                                                                                                                                                                     gTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTgcgc                                                                                                                                                                                                                 <  1:857148/54‑1 (MQ=255)                                                                                                                                                                                                                     gTAATGATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAacac                                                  >  1:43685/1‑213 (MQ=255)                                                                                                                                                                                                                          gATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGc                                                                         <  1:90196/185‑1 (MQ=255)                                                                                                                                                                                                                          gATTAGTTAGCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACGTCAAATGTTTGATTTAACTTCAAATAAAACTGCAtt       >  1:153613/1‑251 (MQ=255)                                                                                                                                                                                                                              agttagCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAGTTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGc                                                              <  1:335716/192‑1 (MQ=255)                                                                                                                                                                                                                              agttagCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACGTCAAATGTTTGATTTAACTTCAAATAAAACTGCAtttctt   >  1:762039/1‑251 (MQ=255)                                                                                                                                                                                                                              agttagCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACGTCAAATGTTTGATTTAACTTCAAATAAAACTGCAtttctt   >  1:266416/1‑251 (MQ=255)                                                                                                                                                                                                                               gttagCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACGTCAAATGTTTGATTTAACTTCAAATAAAACTGCAtttcttt  >  1:53481/1‑251 (MQ=255)                                                                                                                                                                                                                                 tagCATGGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCtg                                                                       >  1:17976/1‑180 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   GGATAATAAAGCTAACATTAGAACAAATTCTCCAAAGCCTGAATTAACACGTAATCCGGTTTATCAAAAAACACGCCGTTTTGATTTTCGCCGTTCATGCCACGTAAGAAACAATAAAACACACCGCCGAAATGGGTTTCATAGTCATAGTTTGCGTCACGTTGTTGTAAATAACGGTGCAATGCCAGCGTGTAAAATAAATATTGCCAGTCGTAATGATTAGTTAGCATCGCTTGCTGAAGGCTTGTGCCGACGTAGTTTTGCGCTTCCACGCCAAGAAAATTGGATTTGTAATCCGCCAAATAATATTTTCCGTTATGATAAAACACCAAATCCATGAAACCGCGTAACATTCCTTTAATCGCTTCAAATTGCAGGGGTTCTGAGGGTAAGTGGTGATGCTGTTGTAACGCTTGATTAAACACCGCCACGTCAAATGTTTGATTTAACTTCAAATAAAACTGCATTTCTTT  >  1/1597526‑1597998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑