Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	299,927	T→C	intergenic (‑31649/+46577)	unknown ← / ← unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Arg‑ACG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	299,927	0	T	C	100.0%	264.2 / NA	77	intergenic (‑31649/+46577)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Arg‑ACG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/29);  total (48/29)

ACGAACCTGTTGGAAAATGGGTTCTGTTTTCATGAATAATTTGACCTTTATTGGTATCTTTTAATATATTTTGACAGCTATTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGTGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCGTTATCTGAATCGGTTATCCGGCCCTTTTTTAGAT  >  1/299688‑300170                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    aCGAACCTGTTGGAAAATGGGTTCTGTTTTCATGAATAATTTGACCTTTATTGGTATCTTTTAATATATTTTGACAGCTATTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCtt                                                                                                                                                                                                                                          <  1:271090/251‑1 (MQ=255)      ccTGTTGGAAAATGGGTTCTGTTTTCATGAATAATTTGACCTTTATTGGTATCTTTTAATATATTTTGACAGCTATTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATg                                                                                                                                                                                                                                     <  1:290247/251‑1 (MQ=255)                                           gACCTTTATTGGTATCTTTTAATATATTTTGACAGCTATTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGAc                                                                                                                                                                                                     >  1:497464/1‑246 (MQ=255)                                                ttATTGGTATCTTTTAATATATTTTGACAGCTATTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCCCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGt                                                                                                                                                                                            >  1:233088/1‑250 (MQ=255)                                                     ggTATCTTTTAATATATTTTGACAGCTATTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCCCTGCGTCAACCa                                                                                                                                                                                      >  1:294342/1‑251 (MQ=255)                                                          cTTTTAATATATTTTGACAGCTATTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGt                                                                                                                                                                                            <  1:107163/240‑1 (MQ=255)                                                          cTTTTAATATATTTTGACAGCTATTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGa                                                                                                                                                                                 >  1:50168/1‑251 (MQ=255)                                                                             gCTATTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAattattatt                                                                                                                                                              >  1:718998/1‑251 (MQ=255)                                                                                aTTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGcc                                                                                                                                                                                                                              >  1:432078/1‑184 (MQ=255)                                                                                aTTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAattattat                                                                                                                                                               <  1:714992/247‑1 (MQ=255)                                                                                 tttGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTcc                                                                                                                                                           >  1:379667/1‑250 (MQ=255)                                                                                 tttGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTcc                                                                                                                                                           >  1:631966/1‑250 (MQ=255)                                                                                  ttGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCcg                                                                                                                                                         >  1:495632/1‑251 (MQ=255)                                                                                        cTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGcgccaa                                                                                                                                                   >  1:217863/1‑251 (MQ=255)                                                                                                ccATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGaa                                                                                                                                                                                >  1:15716/1‑214 (MQ=255)                                                                                                ccATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGa                                                                                                                                           >  1:332158/1‑251 (MQ=255)                                                                                                ccATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGa                                                                                                                                           >  1:277229/1‑251 (MQ=255)                                                                                                ccATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGa                                                                                                                                           >  1:767154/1‑251 (MQ=255)                                                                                                  aTTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAAcc                                                                                                                                                                                       <  1:177239/205‑1 (MQ=255)                                                                                                  aTTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGaa                                                                                                                                                                                <  1:746831/212‑1 (MQ=255)                                                                                                  aTTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGAtt                                                                                                                                         >  1:804485/1‑251 (MQ=255)                                                                                                  aTTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGAtt                                                                                                                                         >  1:489648/1‑251 (MQ=255)                                                                                                  aTTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGAtt                                                                                                                                         >  1:93666/1‑251 (MQ=255)                                                                                                     aaaTAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAattattat                                                                                                                                                               >  1:225221/1‑226 (MQ=255)                                                                                                     aaaTAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCaa                                                                                                                                      >  1:185407/1‑251 (MQ=255)                                                                                                              cttctAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCTGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCa                                                                                                                             >  1:837826/1‑251 (MQ=255)                                                                                                              cttctAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCa                                                                                                                             >  1:170007/1‑251 (MQ=255)                                                                                                                     gggTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAAttt                                                                                                                       >  1:398818/1‑250 (MQ=255)                                                                                                                            aaaaTAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGaaa                                                                                                                                                                      >  1:543739/1‑196 (MQ=255)                                                                                                                                    cTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGa                                                                                                       <  1:49201/251‑1 (MQ=255)                                                                                                                                      aaaGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATg                                                                                                     <  1:522564/251‑1 (MQ=255)                                                                                                                                         gCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATg                                                                                                                                                                                                                                     <  1:82499/120‑1 (MQ=255)                                                                                                                                         gCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAAt                                                                                                  >  1:283119/1‑251 (MQ=255)                                                                                                                                              aTTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAg                                                                                             >  1:686641/1‑251 (MQ=255)                                                                                                                                                tCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGcc                                                                                           <  1:339520/251‑1 (MQ=255)                                                                                                                                                      gTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGaa                                                                                                                                                                                >  1:21375/1‑160 (MQ=255)                                                                                                                                                       ttCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAgg                                                                                    >  1:798780/1‑251 (MQ=255)                                                                                                                                                              ttttGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTAt                                                                             <  1:610720/251‑1 (MQ=255)                                                                                                                                                               tttGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATc                                                                            <  1:535727/251‑1 (MQ=255)                                                                                                                                                               tttGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATc                                                                            <  1:754402/251‑1 (MQ=255)                                                                                                                                                               tttGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATc                                                                            <  1:521882/251‑1 (MQ=255)                                                                                                                                                               tttGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATc                                                                            <  1:500498/251‑1 (MQ=255)                                                                                                                                                                ttGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCa                                                                           <  1:812450/251‑1 (MQ=255)                                                                                                                                                                     gATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCa                                                                      >  1:771310/1‑251 (MQ=255)                                                                                                                                                                      aTTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCa                                                                      >  1:389085/1‑250 (MQ=255)                                                                                                                                                                       ttATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGaa                                                                                                                                                                                <  1:515582/143‑1 (MQ=255)                                                                                                                                                                       ttATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAg                                                                                     >  1:857228/1‑234 (MQ=255)                                                                                                                                                                       ttATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACg                                                                    >  1:273782/1‑251 (MQ=255)                                                                                                                                                                          tGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCAt                                                                 <  1:528385/251‑1 (MQ=255)                                                                                                                                                                           gCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCa                                                                  <  1:167780/249‑1 (MQ=255)                                                                                                                                                                           gCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATa                                                                >  1:466572/1‑251 (MQ=255)                                                                                                                                                                              aCTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGAc                                                                                                                                                                                                     <  1:646309/115‑1 (MQ=255)                                                                                                                                                                              aCTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGAc                                                                                                                                                                                                     <  1:399261/115‑1 (MQ=255)                                                                                                                                                                                  aTTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCa                                                          >  1:756426/1‑250 (MQ=255)                                                                                                                                                                                  aTTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCa                                                          >  1:308649/1‑250 (MQ=255)                                                                                                                                                                                  aTTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCa                                                          >  1:620829/1‑250 (MQ=255)                                                                                                                                                                                             tGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCacaac                                              <  1:139565/251‑1 (MQ=255)                                                                                                                                                                                                     gCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCa                                                                      >  1:509404/1‑219 (MQ=255)                                                                                                                                                                                                     gCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCAcaacaa                                            >  1:173460/1‑245 (MQ=255)                                                                                                                                                                                                       aCGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCg                                    <  1:318591/251‑1 (MQ=255)                                                                                                                                                                                                          aTCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAAc                                                                                                                   >  1:400998/1‑169 (MQ=255)                                                                                                                                                                                                          aTCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCa                                                                      <  1:55664/214‑1 (MQ=255)                                                                                                                                                                                                           tCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGt                                                                                                                                                                             <  1:383028/110‑1 (MQ=255)                                                                                                                                                                                                           tCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGa                                                                                                       >  1:237817/1‑180 (MQ=255)                                                                                                                                                                                                           tCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATc                                                                                                 >  1:595073/1‑186 (MQ=255)                                                                                                                                                                                                           tCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATc                                                                            <  1:363485/207‑1 (MQ=255)                                                                                                                                                                                                           tCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATc                                                                            >  1:153361/1‑207 (MQ=255)                                                                                                                                                                                                           tCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCGTTAt                                >  1:377462/1‑251 (MQ=255)                                                                                                                                                                                                           tCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCGTTAt                                >  1:398342/1‑251 (MQ=255)                                                                                                                                                                                                            ccccAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCAt                                                                                                                                                                                     >  1:153106/1‑101 (MQ=255)                                                                                                                                                                                                            ccccAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTcaccac                                                 <  1:427807/233‑1 (MQ=255)                                                                                                                                                                                                            ccccAAACCGGGCGAAATTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCGTTAt                                >  1:354049/1‑250 (MQ=255)                                                                                                                                                                                                                          aaaTTTCCCTCGCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCGTTATCTGAATCGGTTATc                  >  1:372915/1‑250 (MQ=255)                                                                                                                                                                                                                                     gCTCACAATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCGTTATCTGAATCGGTTATCCGGCCCtttttt      >  1:204024/1‑251 (MQ=255)                                                                                                                                                                                                                                        cacaATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCGTTATCTGAATCGGTTATCCGGccc            >  1:306736/1‑242 (MQ=255)                                                                                                                                                                                                                                         acaATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCGTTATCTGAATCGGTTATCCGGCCCTTTTTTAGAt  <  1:163917/251‑1 (MQ=255)                                                                                                                                                                                                                                         acaATGGCGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCGTTATCTGAATCGGTTATCCGGCCCTTTTTTAGAt  <  1:543933/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    ACGAACCTGTTGGAAAATGGGTTCTGTTTTCATGAATAATTTGACCTTTATTGGTATCTTTTAATATATTTTGACAGCTATTTGTAGCTTTTGTGCCATTAAATAGTATCTTCTAAGGGTAAAAAAATAGGCTAAAGCCTTATTCTATCGTTCTAAGTTTTGCCGATTATGCCACTTATTTGTAACTTTGACAGCGGCACGATCCCCAAACCGGGCGAAATTTCCCTCGCTCACAATGGTGTGCTTTTCTTAGATGAGTTGCCCGAATTTGAACGCAAAGTGCTGGACGCGCTGCGTCAACCATTGGAAAGTGGCGAAATTATTATTTCCCGCGCCAACGCCAAGATTCAATTTCCGGCAAAATTTCAACTGATTGCCGCGATGAATCCCAGCCCGACAGGGCATTATCAAGGCACGCATAATCGCACCTCACCACAACAAGTTATGCGTTATCTGAATCGGTTATCCGGCCCTTTTTTAGAT  >  1/299688‑300170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑