Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,215,820	G→A	intergenic (‑105466/‑90649)	unknown ← / → unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,215,820	0	G	A	100.0%	287.0 / NA	82	intergenic (‑105466/‑90649)	unknown/unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/47);  total (35/47)

AAGAATTCCCCGTCGCTACCAACAAAGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTGCTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAGTGGAAATTTTGGTTGGGTTAATGACGGATAAAAACGTCACAAAATTGGTTTCCGGT  >  1/1215576‑1216064                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     aagaaTTCCCCGTCGCTACCAACAAAGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACtgatg                                                                                                                                                                                                                                                <  1:413745/251‑1 (MQ=255)             tCGCTACCAACAAAGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGt                                                                                                                                                                                                                                      >  1:264966/1‑249 (MQ=255)             tCGCTACCAACAAAGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTAt                                                                                                                                                                                                                                    <  1:796304/251‑1 (MQ=255)               gCTACCAACAAAGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATgg                                                                                                                                                                                                                                  >  1:193894/1‑251 (MQ=255)                   ccAACAAAGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGgtgtg                                                                                                                                                                                                                              >  1:150986/1‑251 (MQ=255)                   ccAACAAAGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGgtgtg                                                                                                                                                                                                                              >  1:44943/1‑251 (MQ=255)                     aacaaAGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGgtg                                                                                                                                                                                                                                >  1:473840/1‑247 (MQ=255)                        aaaGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCAc                                                                                                                                                                                                                         <  1:655382/251‑1 (MQ=255)                         aaGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCAcc                                                                                                                                                                                                                        <  1:871301/251‑1 (MQ=255)                              cgcTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTcc                                                                                                                                                                                                                   <  1:368654/251‑1 (MQ=255)                                cTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCtt                                                                                                                                                                                                                 <  1:80082/251‑1 (MQ=255)                                cTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCtt                                                                                                                                                                                                                 >  1:557956/1‑251 (MQ=255)                                cTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCt                                                                                                                                                                                                                  <  1:792212/250‑1 (MQ=255)                                   cACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTAtg                                                                                                                                                                                                              <  1:26169/251‑1 (MQ=255)                                   cACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTAtg                                                                                                                                                                                                              <  1:719381/251‑1 (MQ=255)                                      tGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGa                                                                                                                                                                                                           <  1:272542/251‑1 (MQ=255)                                      tGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGa                                                                                                                                                                                                           <  1:586524/251‑1 (MQ=255)                                            tgtTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGatcatc                                                                                                                                                                                                      <  1:69787/250‑1 (MQ=255)                                               tttCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACg                                                                                                                                                                                                  >  1:856586/1‑251 (MQ=255)                                                ttcttcAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACgg                                                                                                                                                                                                 <  1:653354/251‑1 (MQ=255)                                                        tttACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATAc                                                                                                                                                                                          <  1:786629/250‑1 (MQ=255)                                                                cTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGgtgt                                                                                                                                                                                                                               >  1:45281/1‑205 (MQ=255)                                                                cTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATa                                                                                                                                                                                           >  1:822426/1‑241 (MQ=255)                                                                cTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGAc                                                                                                                                                                                  >  1:470121/1‑250 (MQ=255)                                                                cTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGAc                                                                                                                                                                                  >  1:832001/1‑250 (MQ=255)                                                                           gAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCtt                                                                                                                                                                                                                 >  1:432013/1‑208 (MQ=255)                                                                           gAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGc                                                                                                                                                                       <  1:542676/250‑1 (MQ=255)                                                                              cATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGta                                                                                                                                                                   >  1:325418/1‑251 (MQ=255)                                                                              cATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGta                                                                                                                                                                   >  1:816768/1‑251 (MQ=255)                                                                               aTCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGtat                                                                                                                                                                  <  1:11792/251‑1 (MQ=255)                                                                                  tGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAAc                                                                                                                                                               <  1:155063/251‑1 (MQ=255)                                                                                  tGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAAc                                                                                                                                                               <  1:880814/251‑1 (MQ=255)                                                                                  tGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAAc                                                                                                                                                               <  1:755854/251‑1 (MQ=255)                                                                                    tCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             <  1:104086/251‑1 (MQ=255)                                                                                    tCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             <  1:260514/251‑1 (MQ=255)                                                                                    tCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             <  1:740782/251‑1 (MQ=255)                                                                                    tCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATCATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             <  1:753868/251‑1 (MQ=255)                                                                                        ccGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGt                                                                                                                                                                    >  1:604177/1‑240 (MQ=255)                                                                                           cATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGgtg                                                                                                                                                                                                                                <  1:889933/177‑1 (MQ=255)                                                                                                cTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCt                                                                                                                                                 <  1:213603/251‑1 (MQ=255)                                                                                                          ccTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACGTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATgcgc                                                                                                                                                  <  1:606014/240‑1 (MQ=255)                                                                                                          ccTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             <  1:772223/229‑1 (MQ=255)                                                                                                          ccTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             >  1:613720/1‑229 (MQ=255)                                                                                                          ccTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTg                                                                                                                                       >  1:445746/1‑251 (MQ=255)                                                                                                                       gCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             >  1:689324/1‑216 (MQ=255)                                                                                                                       gCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTaa                                                                                                                          >  1:476496/1‑251 (MQ=255)                                                                                                                            atatCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGa                                                                                                                                                                                                                                           <  1:38382/133‑1 (MQ=255)                                                                                                                            atatCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTAtg                                                                                                                                                                                                              <  1:349291/162‑1 (MQ=255)                                                                                                                            atatCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCaa                                                                                                                                                                                              >  1:17060/1‑178 (MQ=255)                                                                                                                            atatCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAAc                                                                                                                                                               <  1:170412/209‑1 (MQ=255)                                                                                                                            atatCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAAc                                                                                                                                                               >  1:900239/1‑209 (MQ=255)                                                                                                                                cAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAaat                                                                                                                 <  1:331084/251‑1 (MQ=255)                                                                                                                                  aaTTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             >  1:769930/1‑205 (MQ=255)                                                                                                                                   aTTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAaataat                                                                                                              <  1:473858/251‑1 (MQ=255)                                                                                                                                   aTTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAaataat                                                                                                              <  1:625956/251‑1 (MQ=255)                                                                                                                                           gCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGgtg                                                                                                                                                                                                                                <  1:402010/129‑1 (MQ=255)                                                                                                                                                   aaTCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAata                                                                                              <  1:773871/251‑1 (MQ=255)                                                                                                                                                   aaTCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGACCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAATGACGTTTTGACCGCCAGCACGCTGTTTAACGATGATTATGCGCTTGCCTTGGTGGATTTTTTATTATTTAGAAAATAATAACGCAAGGATAAatt                                                                                              <  1:513683/251‑2 (MQ=255)                                                                                                                                                    aTCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             <  1:142311/187‑1 (MQ=255)                                                                                                                                                      cTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             >  1:824315/1‑185 (MQ=255)                                                                                                                                                         gTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             <  1:521294/182‑1 (MQ=255)                                                                                                                                                                 gCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTAt                                                                                 <  1:689759/250‑1 (MQ=255)                                                                                                                                                                     cAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTc                                                                             <  1:106031/250‑1 (MQ=255)                                                                                                                                                                              ggCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCAcc                                                                                                                                                                                                                        >  1:71716/1‑102 (MQ=255)                                                                                                                                                                              ggCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAAc                                                                                                                                                               >  1:110820/1‑159 (MQ=255)                                                                                                                                                                                      gcgcGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAAt                                                                                       >  1:146429/1‑223 (MQ=255)                                                                                                                                                                                       cgcgATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGTGCAGGAAg                                                          <  1:345957/251‑1 (MQ=255)                                                                                                                                                                                         cgATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGa                                                                                                                                                                                                                                           >  1:287682/1‑72 (MQ=255)                                                                                                                                                                                         cgATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTAtg                                                                                                                                                                                                              <  1:318776/101‑1 (MQ=255)                                                                                                                                                                                         cgATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAGTg                                                        >  1:493651/1‑251 (MQ=255)                                                                                                                                                                                           aTAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             >  1:509526/1‑148 (MQ=255)                                                                                                                                                                                           aTAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAGTGGa                                                      >  1:806125/1‑251 (MQ=255)                                                                                                                                                                                                  cTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATCCGTTTTGACCGCCAGCACGCTGTATAACga                                                                                                                                                             <  1:770502/141‑1 (MQ=255)                                                                                                                                                                                                           gTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAGTGGAAATTTTGGTTGGGTTa                                      >  1:703748/1‑251 (MQ=255)                                                                                                                                                                                                           gTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAGTGGAAATTTTGGTTGGGTTa                                      >  1:472900/1‑251 (MQ=255)                                                                                                                                                                                                               cATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAGTGGAAATTTTGGTTGGGTTAATGa                                  <  1:564589/251‑1 (MQ=255)                                                                                                                                                                                                                aTTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAACTTGGTATTGTCGGCGCCATGGCGCAGGAAGTGGAAATTTTGGTTGGGTTAATGa                                  >  1:590905/1‑250 (MQ=255)                                                                                                                                                                                                                      aTCAGATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAg                                                          <  1:810644/220‑1 (MQ=255)                                                                                                                                                                                                                          gATTCACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAat                                                                                               <  1:810915/179‑1 (MQ=255)                                                                                                                                                                                                                              cACCAAAAAGTGAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAGTGGAAATTTTGGTTGGGTTAATGACGGATAAAAACGTca                   >  1:191124/1‑251 (MQ=255)                                                                                                                                                                                                                                         gAATGACGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAGTGGAAATTTTGGTTGGGTTAATGACGGATAAAAACGTCACAAAATTGGtt        >  1:457545/1‑251 (MQ=255)                                                                                                                                                                                                                                               cGCCGTACTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAGTGGAAATTTTGGTTGGGTTAATGACGGATAAAAACGTCACAAAATTGGTTTCCGGt  <  1:495925/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     AAGAATTCCCCGTCGCTACCAACAAAGCGCGCTTCACTGCCGGTGTTTTCTTCAGTTTACAAGCTTTAAATGTGGAGCATCTGTCTTCCGCATTACTGTTTGAAACCTCGGAACGGCAGCGTTATATCAAATTATCCCGCATTGAAAATCTGGTACATTGGCTGCAAAAACAAGGCATTGAGCGCGATAGTTTCTTGGAAATGTATCATTCCGATCAGATTCACCAAAAAGTGAATGACGCCGTGCTGATGACGGAAGAGTATGGTGTGTTCACCTTTCCTTATGTGATCATCAACGGCAAATACGTTTTGACCGCCAGCACGCTGTATAACGATGATTATGCGCTCGCCGTGTTGGATTTTTTAGTAAATAGAAAATAATAACGCAAGGATAAATATGAAAATTGGTATTGTCGGCGCCATGGCGCAGGAAGTGGAAATTTTGGTTGGGTTAATGACGGATAAAAACGTCACAAAATTGGTTTCCGGT  >  1/1215576‑1216064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑