Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,147,721	T→C	intergenic (‑37367/‑158748)	unknown ← / → unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,147,721	0	T	C	100.0%	256.1 / NA	75	intergenic (‑37367/‑158748)	unknown/unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/40);  total (35/40)

GTTTACCTGCGCCGATTGCCGGATGCGCTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCTTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGGTAGTAATCGCTTTTCAGCACGCCGTGTCCCCAGGCTTCGCCCGTCATCCAAAAGGGTTCAC  >  1/1147475‑1147963                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   gtttACCTGCGCCGATTGCCGGATGCGCTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGCAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGATAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTgg                                                                                                                                                                                                                                                >  1:845831/1‑251 (MQ=255)  tttACCTGCGCCGATTGCCGGATGCGCTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGc                                                                                                                                                                                                                                               <  1:433021/251‑1 (MQ=255)  tttACCTGCGCCGATTGCCGGATGCGCTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGc                                                                                                                                                                                                                                               <  1:501369/251‑1 (MQ=255)      ccTGCGCCGATTGCCGGATGCGCTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTAt                                                                                                                                                                                                                                            >  1:10035/1‑250 (MQ=255)      ccTGCGCCGATTGCCGGATGCGCTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTAt                                                                                                                                                                                                                                            >  1:841515/1‑250 (MQ=255)        tGCGCCGATTGCCGGATGCGCTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTAAAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCg                                                                                                                                                                                                                                         <  1:821429/251‑1 (MQ=255)               aTTGCCGGATGCGCTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGAt                                                                                                                                                                                                                                   <  1:555355/250‑1 (MQ=255)                        tGCGCTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACg                                                                                                                                                                                                                         <  1:736187/251‑1 (MQ=255)                          cgcTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACgg                                                                                                                                                                                                                        <  1:212303/250‑1 (MQ=255)                              tGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATc                                                                                                                                                                                                                                           <  1:803209/227‑1 (MQ=255)                                         ggCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGaaa                                                                                                                                                                                                        >  1:213400/1‑251 (MQ=255)                                         ggCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGaaa                                                                                                                                                                                                        >  1:803662/1‑251 (MQ=255)                                            gAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACGGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGt                                                                                                                                                                                                     >  1:449040/1‑251 (MQ=255)                                              gtgtTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTaa                                                                                                                                                                                                   >  1:771904/1‑251 (MQ=255)                                                        gTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACatat                                                                                                                                                                                         >  1:837953/1‑251 (MQ=255)                                                           ggTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTc                                                                                                                                                                                                <  1:54862/241‑1 (MQ=255)                                                            gTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGa                                                                                                                                                                                      <  1:33592/250‑1 (MQ=255)                                                             tGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGaaa                                                                                                                                                                                    <  1:124374/251‑1 (MQ=255)                                                                gCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATc                                                                                                                                                                                  <  1:447136/250‑1 (MQ=255)                                                                   gggCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGAt                                                                                                                                                                                                                                   >  1:541439/1‑198 (MQ=255)                                                                   gggCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTAt                                                                                                                                                                                                                    <  1:385942/213‑1 (MQ=255)                                                                         gtttgtCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTg                                                                                                                                                                        >  1:803849/1‑251 (MQ=255)                                                                                     ttGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTc                                                                                                                                                            <  1:21990/251‑1 (MQ=255)                                                                                       gtgCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGtt                                                                                                                                                             >  1:155993/1‑248 (MQ=255)                                                                                          ccGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGAt                                                                                                                                                        <  1:868521/250‑1 (MQ=255)                                                                                          ccGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATa                                                                                                                                                       <  1:308465/251‑1 (MQ=255)                                                                                                   tGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGAt                                                                                                                                               >  1:167912/1‑250 (MQ=255)                                                                                                       aaTTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAAtg                                                                                                                                          <  1:843991/251‑1 (MQ=255)                                                                                                       aaTTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAAtg                                                                                                                                          <  1:355228/251‑1 (MQ=255)                                                                                                        aTTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTc                                                                                                                                                            <  1:21113/232‑1 (MQ=255)                                                                                                              atCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGa                                                                                                                                   >  1:91740/1‑251 (MQ=255)                                                                                                                  gAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCg                                                                                                                                                                                                                                         >  1:286974/1‑145 (MQ=255)                                                                                                                    aCGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAAt                                                                                                                             >  1:269915/1‑251 (MQ=255)                                                                                                                     cgcgTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAAtg                                                                                                                                          <  1:505168/237‑1 (MQ=255)                                                                                                                       cgTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATcc                                                                                                                           <  1:186691/250‑1 (MQ=255)                                                                                                                                 aTCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCAttttt                                                                                                                <  1:215841/251‑1 (MQ=255)                                                                                                                                     ggTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCAttttt                                                                                                                >  1:408971/1‑247 (MQ=255)                                                                                                                                                 tGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGaaa                                                                                                 <  1:901759/250‑1 (MQ=255)                                                                                                                                                 tGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGaaa                                                                                                 <  1:4585/250‑1 (MQ=255)                                                                                                                                                    gTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTc                                                                                                                                                            >  1:871611/1‑188 (MQ=255)                                                                                                                                                    gTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATacac                                                                                                                                                    <  1:487830/196‑1 (MQ=255)                                                                                                                                                    gTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTggg                                                                                                                                      <  1:857051/210‑1 (MQ=255)                                                                                                                                                    gTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAg                                                                                                <  1:50356/248‑1 (MQ=255)                                                                                                                                                    gTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCg                                                                                             >  1:642758/1‑251 (MQ=255)                                                                                                                                                    gTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCg                                                                                             >  1:423298/1‑251 (MQ=255)                                                                                                                                                             cccGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATc                                                                                    <  1:849762/251‑1 (MQ=255)                                                                                                                                                             cccGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATc                                                                                    <  1:252792/251‑1 (MQ=255)                                                                                                                                                               cGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCAt                                                                                  >  1:527104/1‑251 (MQ=255)                                                                                                                                                                  ccGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGc                                                                               <  1:808360/251‑1 (MQ=255)                                                                                                                                                                          aTCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGc                                                                       <  1:650153/251‑1 (MQ=255)                                                                                                                                                                              ccGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATc                                                                                    >  1:563867/1‑234 (MQ=255)                                                                                                                                                                                 tAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACat                                                                                                                                                                                           >  1:201022/1‑128 (MQ=255)                                                                                                                                                                                 tAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCa                                                                                                                                                                 >  1:572276/1‑154 (MQ=255)                                                                                                                                                                                     aGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAAt                                                                                                                                                                                   <  1:629190/132‑1 (MQ=255)                                                                                                                                                                                     aGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGgtag                                                            >  1:829188/1‑251 (MQ=255)                                                                                                                                                                                      gATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTg                                                                                       >  1:504447/1‑223 (MQ=255)                                                                                                                                                                                      gATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGgtagt                                                           >  1:167002/1‑251 (MQ=255)                                                                                                                                                                                        tttGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGaa                                                                                                  <  1:244831/210‑1 (MQ=255)                                                                                                                                                                                           gTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGgt                                                              >  1:192165/1‑243 (MQ=255)                                                                                                                                                                                                    ccGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGGTAGTAATCGCTTTTCAGc                                             <  1:284145/251‑1 (MQ=255)                                                                                                                                                                                                          ccAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGGTAGTAATCGCTTTTCAGCACGCCg                                       <  1:522303/251‑1 (MQ=255)                                                                                                                                                                                                            aGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAAtg                                                                                                                                          >  1:568884/1‑150 (MQ=255)                                                                                                                                                                                                            aGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGGTAGTAATCGCTTTTCAGCACGCCgtg                                     >  1:682508/1‑251 (MQ=255)                                                                                                                                                                                                               aataaCAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATcc                                                                                                                           >  1:86875/1‑162 (MQ=255)                                                                                                                                                                                                               aataaCAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGaaa                                                                                                 <  1:658637/188‑1 (MQ=255)                                                                                                                                                                                                               aataaCAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGGTAGTAATCGCTTTTCAGCACGCCGTGTcc                                  <  1:850028/251‑1 (MQ=255)                                                                                                                                                                                                                 taaCAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGtt                                                                                                                                                                          >  1:27010/1‑113 (MQ=255)                                                                                                                                                                                                                 taaCAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGaaa                                                                                                 <  1:368722/186‑1 (MQ=255)                                                                                                                                                                                                                      ggTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATac                                                                                                                                                      >  1:268949/1‑128 (MQ=255)                                                                                                                                                                                                                       gTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGGTAGTAATCGCTTTTCAGCACGCCGTGTCCCCAGGc                            <  1:601555/249‑1 (MQ=255)                                                                                                                                                                                                                                         tttCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGGTAGTAATCGCTTTTCAGCACGCCGTGTCCCCAGGCTTCGCCCGTCATCCAAAAg         <  1:140950/250‑1 (MQ=255)                                                                                                                                                                                                                                         tttCGCGTTGTTGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGGTAGTAATCGCTTTTCAGCACGCCGTGTCCCCAGGCTTCGCCCGTCATCCAAAAg         <  1:729998/250‑1 (MQ=255)                                                                                                                                                                                                                                               gttgttGCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGaa                                                                                                  >  1:623155/1‑155 (MQ=255)                                                                                                                                                                                                                                                ttgttgCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGGTAGTAATCGCTTTTCAGCACGCCGTGTCCCCAGGCTTCGCCCGTCATCCAAAAGGGTTCAc  >  1:8071/1‑250 (MQ=255)                                                                                                                                                                                                                                                  gttgCCTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTgg                                                               >  1:577238/1‑187 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   GTTTACCTGCGCCGATTGCCGGATGCGCTTGGCGGAAGCGGGCGAGTGTTTGCCAGTGGGTGAGCAGGGCTTGTTTGTCGCCGTTTGTGCCGAGGTCCTGCCAATTCATATCGGAACGCGTGCCTTGCATCGGGTCGGAACCGGTGGGTCCAAAGTCCCGTCCGCTTTCATCGCCGTAATAGATTTGTACAGCGCCCGGCGCCAGTAATAACAGGTTAGCGGCGATTTTTTGTTTCGCGTTGTTGCTTTGGCTATCCGAATGATAGAACAAACGGGTATCGTGGGAGGAAAGGTAACTCAACATATTGAAATCTTTCAGTTTGTCGCTCATGTTCTGATACACGGGATCAATGTGGGCGAAACAATCCAACGCATTTTTTGCTTGGTCCTGAAAGTCGAAATTGATCATGGCGTCGAAGCCGTTTTGGTAGTAATCGCTTTTCAGCACGCCGTGTCCCCAGGCTTCGCCCGTCATCCAAAAGGGTTCAC  >  1/1147475‑1147963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑