Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,115,682	T→C	intergenic (‑5328/‑190787)	unknown ← / → unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,115,682	0	T	C	100.0%	233.3 / NA	71	intergenic (‑5328/‑190787)	unknown/unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/41);  total (30/41)

TATCCAATACTTGATTCTGTTTATTCGGTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGTACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATG  >  1/1115435‑1115915                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          tatCCAATACTTGATTCTGTTTATTCGGTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGcaca                                                                                                                                                                                                                                        >  1:1610/1‑251 (MQ=255)  atCCAATACTTGATTCTGTTTATTCGGTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGcaca                                                                                                                                                                                                                                        >  1:371875/1‑250 (MQ=255)  atCCAATACTTGATTCTGTTTATTCGGTTCAAAAGCATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGcaca                                                                                                                                                                                                                                        >  1:317682/1‑250 (MQ=255)         aCTTGATTCTGTTTATTCGGTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAAt                                                                                                                                                                                                                                >  1:594123/1‑251 (MQ=255)         aCTTGATTCTGTTTATTCGGTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAAt                                                                                                                                                                                                                                >  1:495661/1‑251 (MQ=255)           ttGATTCTGTTTATTCGGTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGc                                                                                                                                                                                                                              <  1:198815/251‑1 (MQ=255)           ttGATTCTGTTTATTCGGTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGc                                                                                                                                                                                                                              <  1:265480/251‑1 (MQ=255)                 cTGTTTATTCGGTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGa                                                                                                                                                                                                                        <  1:599958/251‑1 (MQ=255)                   gTTTATTCGGTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACa                                                                                                                                                                                                                      >  1:601243/1‑251 (MQ=255)                            gTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCcaca                                                                                                                                                                                                             >  1:318716/1‑251 (MQ=255)                            gTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCcaca                                                                                                                                                                                                             >  1:236946/1‑251 (MQ=255)                            gTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCcaca                                                                                                                                                                                                             >  1:682878/1‑251 (MQ=255)                             ttCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACAt                                                                                                                                                                                                            <  1:714927/251‑1 (MQ=255)                                   aGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGAttt                                                                                                                                                                                                      >  1:208611/1‑251 (MQ=255)                                    gTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGAtttt                                                                                                                                                                                                     >  1:119039/1‑251 (MQ=255)                                         atgCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTg                                                                                                                                                                                                 <  1:506589/250‑1 (MQ=255)                                                  gAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATatgat                                                                                                                                                                                       >  1:460981/1‑251 (MQ=255)                                                   aaaTCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATatgatg                                                                                                                                                                                      <  1:664518/251‑1 (MQ=255)                                                   aaaTCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATatgatg                                                                                                                                                                                      <  1:656261/251‑1 (MQ=255)                                                   aaaTCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATatgatg                                                                                                                                                                                      <  1:608890/251‑1 (MQ=255)                                                   aaaTCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATatgat                                                                                                                                                                                       <  1:830027/250‑1 (MQ=255)                                                    aaTCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGt                                                                                                                                                                                     <  1:720300/251‑1 (MQ=255)                                                     aTCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATg                                                                                                                                                                                                                               >  1:179734/1‑208 (MQ=255)                                                        aaaCAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAAcc                                                                                                                                                                                 <  1:500013/251‑1 (MQ=255)                                                        aaaCAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAAcc                                                                                                                                                                                 <  1:571526/251‑1 (MQ=255)                                                         aacaacGCGAATTCCAGCTGTCGCAAAACAAACTTCACCGCTTGGAAATTTTTCGCTTTGAGTAATTGGTTCTGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAAccc                                                                                                                                                                                <  1:672909/251‑1 (MQ=255)                                                         aacaacGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAAccc                                                                                                                                                                                <  1:613182/251‑1 (MQ=255)                                                         aacaacGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAAccc                                                                                                                                                                                <  1:818277/251‑1 (MQ=255)                                                             acGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAAcc                                                                                                                                                                                 <  1:594793/246‑1 (MQ=255)                                                             acGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATg                                                                                                                                                                             >  1:893320/1‑250 (MQ=255)                                                                             aCGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAAc                                                                                                                                                             <  1:99586/250‑1 (MQ=255)                                                                                 aaaaCAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGt                                                                                                                                                        <  1:19424/251‑1 (MQ=255)                                                                                 aaaaCAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGt                                                                                                                                                        <  1:98363/251‑1 (MQ=255)                                                                                  aaacaaacATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTa                                                                                                                                                       >  1:731437/1‑251 (MQ=255)                                                                                      aaacaTCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGa                                                                                                                                                   <  1:170549/251‑1 (MQ=255)                                                                                       aacctCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGAc                                                                                                                                                  <  1:806278/247‑1 (MQ=255)                                                                                       aacaTCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGAc                                                                                                                                                  <  1:204950/251‑1 (MQ=255)                                                                                        acaTCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCCGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACt                                                                                                                                                 <  1:515605/251‑1 (MQ=255)                                                                                                         tttttCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGaataa                                                                                                                                <  1:531348/251‑1 (MQ=255)                                                                                                         tttttCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGaataa                                                                                                                                <  1:213911/251‑1 (MQ=255)                                                                                                          cctcggatttgtgtataagagacAGTTTCTCTTTTGGTAACGGTTCCCCCGTTTGATAATGACCGGAAAGAAACTCCAACGCTTCTTCTTCCCAACACCGTTTTTCGATAAATTGACTGGGCAATTCCTCGACATCCCAAGACACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGaataa                                                                                                                                <  1:515999/228‑1 (MQ=255)                                                                                                                    gAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGa                                                                                                                                                   <  1:737304/221‑1 (MQ=255)                                                                                                                    gAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGACTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGAt                                                                                                                     >  1:741951/1‑251 (MQ=255)                                                                                                                       tAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCGCCCGTTTGATAATGACCGGAAATAAACTGCATCGCTTCTTCTTCCCAGCGCCAGTTTTCCATAAATTGACTTGGCAATTCCCCGGCATCCCAAGGCGCACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCAc                                                                                                                  <  1:523536/251‑1 (MQ=255)                                                                                                                        aaTTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGccc                                                                                                                                                                          >  1:526206/1‑194 (MQ=255)                                                                                                                        aaTTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGt                                                                                                                                                        >  1:602917/1‑212 (MQ=255)                                                                                                                            gggTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAg                                                                                                              >  1:226683/1‑250 (MQ=255)                                                                                                                                  gTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGcc                                                                                                                                                                                                                             <  1:334513/133‑1 (MQ=255)                                                                                                                                  gTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTg                                                                                                                                                                                                 >  1:570134/1‑161 (MQ=255)                                                                                                                                     tctcTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTg                                                                                                    >  1:323268/1‑251 (MQ=255)                                                                                                                                                   ggTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTaa                                                                                      >  1:531031/1‑251 (MQ=255)                                                                                                                                                         cccGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAgg                                                                                 >  1:293220/1‑250 (MQ=255)                                                                                                                                                            gTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGaat                                                                                                                                  <  1:492881/198‑1 (MQ=255)                                                                                                                                                                           gAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATatgatg                                                                                                                                                                                      >  1:814375/1‑131 (MQ=255)                                                                                                                                                                           gAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGa                                                                >  1:80021/1‑249 (MQ=255)                                                                                                                                                                                aaaCTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAAc                                                                                                                                                             >  1:5047/1‑151 (MQ=255)                                                                                                                                                                                   cTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATatgat                                                                                                                                                                                       >  1:568235/1‑122 (MQ=255)                                                                                                                                                                                    tGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGAt                                                     <  1:703214/251‑1 (MQ=255)                                                                                                                                                                                         cGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGAc                                                                                                                                                  >  1:753930/1‑153 (MQ=255)                                                                                                                                                                                            ttcttcttcCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTAc                                                            <  1:759858/236‑1 (MQ=255)                                                                                                                                                                                              cttcttcCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGcga                                           <  1:882970/251‑1 (MQ=255)                                                                                                                                                                                                   tcCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCgacgac                                       >  1:833495/1‑250 (MQ=255)                                                                                                                                                                                                        gCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATg                                                                                                                                                                                                                               >  1:550427/1‑61 (MQ=255)                                                                                                                                                                                                         cACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGa                                                                                                                                                   <  1:799677/136‑1 (MQ=255)                                                                                                                                                                                                         cACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATAc                                 <  1:27314/250‑1 (MQ=255)                                                                                                                                                                                                               ttttCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGtc                          <  1:670527/251‑1 (MQ=255)                                                                                                                                                                                                                    cATAAATTGACTTGGCAATTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGtccatcc                     <  1:472168/251‑1 (MQ=255)                                                                                                                                                                                                                                     aTTCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTa    >  1:713173/1‑251 (MQ=255)                                                                                                                                                                                                                                       tCCACGGCATCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATg  <  1:89323/251‑1 (MQ=255)                                                                                                                                                                                                                                                tCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAAccc                                                                                                                                                                                >  1:101988/1‑68 (MQ=255)                                                                                                                                                                                                                                                tCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGAc                                                               <  1:355173/181‑1 (MQ=255)                                                                                                                                                                                                                                                tCCCAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGcga                                           <  1:481825/201‑1 (MQ=255)                                                                                                                                                                                                                                                 cccAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATatgat                                                                                                                                                                                       >  1:388761/1‑60 (MQ=255)                                                                                                                                                                                                                                                  ccAAGGCACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAAt                                                                                    <  1:626860/158‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          TATCCAATACTTGATTCTGTTTATTCGGTTCAAAAGTATGATGCAAACGGAAATCAAACAACGCGAATTCCAGCTGACGCAAAACAAACATCGCCGCTTGGAAATTTTTCGCTTTGAGTAATTGGGTCAGTTTCTCTTTTGGTAACGGTTCACCCGTTTGATAATGACCGGAAATAAACTGCAACGCTTCTTCTTCCCAGCACCAGTTTTCCATAAATTGACTTGGCAATTCCACGGCATCCCAAGGTACACCGTTAATGCCGGCGACATCCGCCACATCGATTTTGGTGAGCATATGATGTAACCCATGCCCGAATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATG  >  1/1115435‑1115915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑