Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,098,586	T→G	intergenic (+10845/+11693)	unknown → / ← unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,098,586	0	T	G	100.0%	238.6 / NA	69	intergenic (+10845/+11693)	unknown/unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/35);  total (34/35)

GCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCATAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGA  >  1/1098342‑1098831                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAAtct                                                                                                                                                                                                                                                  >  1:902089/1‑250 (MQ=255)    aaTTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATa                                                                                                                                                                                                                                              >  1:169400/1‑251 (MQ=255)         gCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc                                                                                                                                                                                                                                          <  1:856113/250‑1 (MQ=255)         gCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc                                                                                                                                                                                                                                          <  1:697904/250‑1 (MQ=255)         gCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc                                                                                                                                                                                                                                          <  1:711361/250‑1 (MQ=255)                       tGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCtttt                                                                                                                                                                                                                           <  1:5998/251‑1 (MQ=255)                                   ccATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAAt                                                                                                                                                                                                               >  1:791054/1‑251 (MQ=255)                                            catGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAg                                                                                                                                                                                                      <  1:863947/251‑1 (MQ=255)                                              tGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGt                                                                                                                                                                                                    >  1:87256/1‑251 (MQ=255)                                              tGGCTATAGTCACCACGCAAATATTCCCACATTTGACGAT‑CCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGtt                                                                                                                                                                                                   >  1:42714/1‑251 (MQ=255)                                                 cTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATAGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGtttt                                                                                                                                                                                                 >  1:808760/1‑251 (MQ=255)                                                                           caTTTGACGATTCCCCACACAGCGAATTGATGGCATATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGTCCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATTCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGTCGAATTGCAGAATCTCATAGGGCAGCGCTTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAac                                                                                                                                                                                 <  1:223358/241‑1 (MQ=255)                                                                           caTTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAg                                                                                                                                                                                                                                             <  1:544933/181‑1 (MQ=255)                                                                           caTTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTg                                                                                                                                                                       >  1:246172/1‑251 (MQ=255)                                                                           caTTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTg                                                                                                                                                                       >  1:59159/1‑251 (MQ=255)                                                                             tttGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAG‑TTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTgcgc                                                                                                                                                                    <  1:176895/251‑1 (MQ=255)                                                                                 aCGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAgg                                                                                                                                                                                                     <  1:883679/215‑1 (MQ=255)                                                                                    aTACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGc                                                                                                                                                                                               >  1:146704/1‑218 (MQ=255)                                                                                    aTACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAac                                                                                                                                                                                 >  1:681012/1‑232 (MQ=255)                                                                                      aCCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc                                                                                                                                                                                                                                          >  1:693253/1‑173 (MQ=255)                                                                                      aCCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAac                                                                                                                                                                                 >  1:753923/1‑230 (MQ=255)                                                                                      aCCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAAt                                                                                                                                                             >  1:474869/1‑250 (MQ=255)                                                                                      aCCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATc                                                                                                                                                            <  1:611164/251‑1 (MQ=255)                                                                                       ccccACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATcc                                                                                                                                                           <  1:792532/251‑1 (MQ=255)                                                                                       ccccACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATc                                                                                                                                                            >  1:103268/1‑250 (MQ=255)                                                                                        cccACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCg                                                                                                                                                          <  1:70520/251‑1 (MQ=255)                                                                                                   aaTGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCAt                                                                                                                                               <  1:352589/251‑1 (MQ=255)                                                                                                                ttCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTg                                                                                                                                                                       >  1:840359/1‑214 (MQ=255)                                                                                                                      tGCAAATCAGCGCGTCGGTGCAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTgcg                                                                                                                             >  1:274646/1‑250 (MQ=255)                                                                                                                                cgcgTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATg                                                                                                                     <  1:182218/248‑1 (MQ=255)                                                                                                                                  cgTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTg                                                                                                                                                                       <  1:217331/196‑1 (MQ=255)                                                                                                                                    tCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGa                                                                                                              >  1:355692/1‑251 (MQ=255)                                                                                                                                       gTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGAtt                                                                                                            <  1:727069/250‑1 (MQ=255)                                                                                                                                                      ctctTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACGCTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGAt                                                                                            <  1:778308/251‑1 (MQ=255)                                                                                                                                                      ctctTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGAt                                                                                            <  1:897735/251‑1 (MQ=255)                                                                                                                                                        ctTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGt                                                                                          >  1:599541/1‑251 (MQ=255)                                                                                                                                                        ctTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGt                                                                                          >  1:188537/1‑251 (MQ=255)                                                                                                                                                        ctTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGt                                                                                          <  1:715262/251‑1 (MQ=255)                                                                                                                                                         ttgttgAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTa                                                                                         <  1:269293/251‑1 (MQ=255)                                                                                                                                                              gAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATgc                                                                                     >  1:506121/1‑250 (MQ=255)                                                                                                                                                                       gTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTg                                                                                                                                                                       <  1:566617/159‑1 (MQ=255)                                                                                                                                                                       gTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGt                                                                                          >  1:61276/1‑236 (MQ=255)                                                                                                                                                                            aTCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACg                                                                      <  1:90624/251‑1 (MQ=255)                                                                                                                                                                              cATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGtt                                                                    >  1:808156/1‑251 (MQ=255)                                                                                                                                                                               aTTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCgaga                                                                                                                       <  1:186027/199‑1 (MQ=255)                                                                                                                                                                               aTTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTc                                                                   <  1:439846/251‑1 (MQ=255)                                                                                                                                                                                               gCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATa                                                                        <  1:194819/230‑1 (MQ=255)                                                                                                                                                                                               gCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAAtt                                                   >  1:609157/1‑251 (MQ=255)                                                                                                                                                                                               gCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAAtt                                                   >  1:787536/1‑251 (MQ=255)                                                                                                                                                                                                cTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAAttt                                                  <  1:892635/251‑1 (MQ=255)                                                                                                                                                                                                 tCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAAttt                                                  >  1:588064/1‑250 (MQ=255)                                                                                                                                                                                                      gCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGc                                                                <  1:431386/231‑1 (MQ=255)                                                                                                                                                                                                      gCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGc                                             >  1:861223/1‑250 (MQ=255)                                                                                                                                                                                                      gCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGc                                             >  1:286339/1‑250 (MQ=255)                                                                                                                                                                                                               cAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGt                                                                                                                                                                 >  1:262881/1‑125 (MQ=255)                                                                                                                                                                                                                aaCAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTc                                  <  1:323557/251‑1 (MQ=255)                                                                                                                                                                                                                    gATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACg                                                                      <  1:126204/211‑1 (MQ=255)                                                                                                                                                                                                                    gATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATcg                              >  1:145214/1‑251 (MQ=255)                                                                                                                                                                                                                    gATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTCTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATcg                              >  1:895675/1‑251 (MQ=255)                                                                                                                                                                                                                        tctctGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATgc                                                                                     <  1:407442/192‑1 (MQ=255)                                                                                                                                                                                                                         ctctGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTc                                                                                                      <  1:799722/174‑1 (MQ=255)                                                                                                                                                                                                                                ccGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAAt                  >  1:857563/1‑251 (MQ=255)                                                                                                                                                                                                                                   gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATgg                >  1:195680/1‑250 (MQ=255)                                                                                                                                                                                                                                   gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATgg                <  1:877740/250‑1 (MQ=255)                                                                                                                                                                                                                                   gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATgg                >  1:538148/1‑250 (MQ=255)                                                                                                                                                                                                                                   gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACCTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACg                                                                      <  1:660440/196‑1 (MQ=255)                                                                                                                                                                                                                                           gCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGc        <  1:348426/250‑1 (MQ=255)                                                                                                                                                                                                                                            cGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGcc       <  1:332588/250‑1 (MQ=255)                                                                                                                                                                                                                                                ttGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGa  >  1:642812/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCATAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGA  >  1/1098342‑1098831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑