Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,030,583	C→A	intergenic (‑27510/+22330)	unknown ← / ← unknown	tRNA‑Lys‑TTT/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,030,583	0	C	A	100.0%	159.3 / NA	48	intergenic (‑27510/+22330)	unknown/unknown	tRNA‑Lys‑TTT/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/25);  total (23/25)

TCAATTTCTGTCCCAAAACCATTAAAGGCTAAACCCGGAGTAATACAGGCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGCATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATACATGTGGCTCGACATTAAACGGAAGCTCATCTGGGTAAAGTTCAGCTATGGCTTTATCATAAGCACGTTGAAAG  >  1/1030346‑1030803                                                                                                                                                                                                                                              |                                                                                                                                                                                                                             tCAATTTCTGTCCCAAAACCATTAAAGGCTAAACCCGGAGTAATACAGGCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATga                                                                                                                                                                                                                 >  1:799183/1‑251 (MQ=255)          gTCCCAAAACCATTAAAGGCTAAACCCGGAGTAATACAGGCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCg                                                                                                                                                                                                        >  1:108021/1‑251 (MQ=255)               aaaaCCATTAAAGGCTAAACCCGGAGTAATACAGGCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAAc                                                                                                                                                                                                   <  1:337839/251‑1 (MQ=255)                     aTTAAAGGCTAAACCCGGAGTAATACAGGCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAat                                                                                                                                                                                             >  1:715096/1‑251 (MQ=255)                           ggCTAAACCCGGAGTAATACAGGCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGa                                                                                                                                                                                        >  1:545299/1‑250 (MQ=255)                                      gAGTAATACAGGCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTttat                                                                                                                                                                            >  1:208599/1‑251 (MQ=255)                                         tAATACAGGCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTttatta                                                                                                                                                                          <  1:62464/250‑1 (MQ=255)                                                ggCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAata                                                                                                                                                                                            <  1:605684/225‑1 (MQ=255)                                                ggCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCG‑CCATTTACGAATGGAATCATCGTCATTACGACTACCGACAACGTAAATATCGACCGGAGGTTTATTATTCACATaa                                                                                                                                                                 >  1:853157/1‑251 (MQ=255)                                                  cAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAag                                                                                                                                                                <  1:588075/251‑1 (MQ=255)                                                            gacTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCg                                                                                                                                                      >  1:824044/1‑251 (MQ=255)                                                             acTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAagag                                                                                                                                                              <  1:805729/242‑1 (MQ=255)                                                             acTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAAtt                                                                                                                                                        <  1:134220/248‑1 (MQ=255)                                                                    gTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCg                                                                                                                                                                                                                       <  1:318566/178‑1 (MQ=255)                                                                    gTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCg                                                                                                                                                                                                        >  1:527332/1‑193 (MQ=255)                                                                    gTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTttat                                                                                                                                                                            <  1:62587/221‑1 (MQ=255)                                                                    gTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGt                                                                                                                                              >  1:683949/1‑251 (MQ=255)                                                                                    cGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACAttttt                                                                                                                               >  1:745445/1‑250 (MQ=255)                                                                                        gCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTc                                                                                                                                             >  1:75982/1‑232 (MQ=255)                                                                                        gCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCgtg                                                                                                                                         <  1:187863/236‑1 (MQ=255)                                                                                                              ttGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAaataca                                                                                                    >  1:138281/1‑251 (MQ=255)                                                                                                                  aaTGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAaatacaatac                                                                                                <  1:346601/251‑1 (MQ=255)                                                                                                                  aaTGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAaatacaatac                                                                                                <  1:714669/251‑1 (MQ=255)                                                                                                                             cATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCAc                                                                                     <  1:99587/251‑1 (MQ=255)                                                                                                                             cATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCAc                                                                                     <  1:769882/251‑1 (MQ=255)                                                                                                                             cATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCAc                                                                                     <  1:405498/251‑1 (MQ=255)                                                                                                                                  tCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACa                                                                                                                                                                                                     <  1:614203/134‑1 (MQ=255)                                                                                                                                  tCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATAccc                                                                                              >  1:220764/1‑237 (MQ=255)                                                                                                                                   cccATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACAttt                                                                                                                                 >  1:736499/1‑201 (MQ=255)                                                                                                                                   cccATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCg                                                                               >  1:610116/1‑251 (MQ=255)                                                                                                                                         aCCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAatat                                                                                                                                                                                           <  1:9378/137‑1 (MQ=255)                                                                                                                                          ccATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTttat                                                                                                                                                                            >  1:474104/1‑151 (MQ=255)                                                                                                                                          ccATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATcc                                                                                                                    <  1:314802/207‑1 (MQ=255)                                                                                                                                                   catattacaccGGTTTCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATACATGTGGCTCGAc                                                               <  1:855515/242‑1 (MQ=255)                                                                                                                                                    gCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTttat                                                                                                                                                                            >  1:195779/1‑141 (MQ=255)                                                                                                                                                               gTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAaatacaatac                                                                                                >  1:670412/1‑206 (MQ=255)                                                                                                                                                                       aTTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAaatacaatac                                                                                                <  1:542357/198‑1 (MQ=255)                                                                                                                                                                                     aGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATACATGTGGCTCGACATTAAACGGAAGCTCATCTGGGt                                        <  1:901292/240‑1 (MQ=255)                                                                                                                                                                                           gttTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATAc                                                                           >  1:258500/1‑199 (MQ=255)                                                                                                                                                                                            ttttACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATACATGTGGCTCGACATTAAACGGAAGCTCATCTGGGTAAAGTTCAGCTATGGCt                       <  1:408327/250‑1 (MQ=255)                                                                                                                                                                                                     gACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGAt                                                                             >  1:620460/1‑187 (MQ=255)                                                                                                                                                                                                          gATCAATACGATGTTTTTTCGCCCATTGACGAATTGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAaatacaatac                                                                                                <  1:285822/163‑1 (MQ=255)                                                                                                                                                                                                          gATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACAttttt                                                                                                                               <  1:221838/132‑1 (MQ=255)                                                                                                                                                                                                          gATCAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATACATGTGGCTCGACATTAAACGGAAGCTCATCTGGGTAAAGTTCAGCTATGGCTTTATCATAAGCACGt        >  1:872068/1‑251 (MQ=255)                                                                                                                                                                                                             cAATACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATACATGTGGCTCGACATTAAACGGAAGCTCATCTGGGTAAAGTTCAGCTATGGCTTTATCATAAGCACGTTGa     >  1:116612/1‑251 (MQ=255)                                                                                                                                                                                                                tACGATGTTTTTTCGCCCATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATACATGTGGCTCGACATTAAACGGAAGCTCATCTGGGTAAAGTTCAGCTATGGCTTTATCATAAGCACGTTGAAAg  <  1:103945/251‑1 (MQ=255)                                                                                                                                                                                                                               cccATTGACGAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATACATGTGGc                                                                    <  1:356904/170‑1 (MQ=255)                                                                                                                                                                                                                                        gAATGGAATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATACATGTGGCTCGACATTAAACGGAAGCTCATCTGGGTAAAGTTCAGCTATGGCTTTATCa                 >  1:544461/1‑212 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                             TCAATTTCTGTCCCAAAACCATTAAAGGCTAAACCCGGAGTAATACAGGCAAAGAGACAGACTATCGGTACTAATAAGCGAGGCGTTGCCATTGACCGTCCTTTTGAATTTGGAATGCGACCGGCATTTTCCCATCACCATATTGCAGCCAGTTGCCGGTATCATGATTTAGGGTAATTAAGCGTTGTTTTACTTTGACCGGATCAATACGATGTTTTTTCGCCCATTGACGAATGGCATCATCGTCATGACGACTACCGACAACGTAAATATCGACCGGAGTTTTATTATTCACATAAGAGAGAATTCGTCCAATGTCCGTGTCACATTTTTCACAATTATCCAACCGGGTAAAATACAATACCCTTCCCGCACTTTGCGATACATGTGGCTCGACATTAAACGGAAGCTCATCTGGGTAAAGTTCAGCTATGGCTTTATCATAAGCACGTTGAAAG  >  1/1030346‑1030803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑