Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	997,376	G→A	intergenic (+47169/+5622)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	997,376	0	G	A	100.0%	231.1 / NA	71	intergenic (+47169/+5622)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/25);  total (46/25)

CGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCGGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGA  >  1/997150‑997620                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     cGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGa                                                                                                                                                                                                                              >  1:358561/1‑251 (MQ=255)             gCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGacac                                                                                                                                                                                                                    >  1:629420/1‑249 (MQ=255)                    aGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTcg                                                                                                                                                                                                            >  1:728064/1‑250 (MQ=255)                                     ttataTTGATGAACGCTATGGCGAGAAATTGTTATCGGAAAGGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCGGGTGGGCAGCTATAAAAACCATCTGGTCACCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGAc                                                                                                                                                                                           <  1:860204/249‑1 (MQ=255)                                     cTATATTGATGACCGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCCCGCTTTCCCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGAc                                                                                                                                                                                           <  1:347739/250‑1 (MQ=255)                                     cTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTcgcc                                                                                                                                                                                                          <  1:478949/235‑1 (MQ=255)                                     cTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGa                                                                                                                                                                                            >  1:161390/1‑249 (MQ=255)                                     cTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGa                                                                                                                                                                                            >  1:198569/1‑249 (MQ=255)                                     cTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGa                                                                                                                                                                                            >  1:396492/1‑249 (MQ=255)                                     cTATATTGATGAACGCTAGGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATAAAAATCATCTGGTCATCCCACGCTTTCCCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGcccc                                                                                                                                                                                               <  1:293938/246‑1 (MQ=255)                                     cTATATTGATAAACGCTATGGCAAAAAAATGTTATCGAAAACGGGGCGCCCGAAAGTAGCAGCCTGTTTAAAGCAGGTCGGCAGCTATAAAAATCATCTGGTCACCCCCCCTTTTCCCCGCCTAGGGCTGCCTGAATATGCCCCCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGAc                                                                                                                                                                                           <  1:535100/250‑1 (MQ=255)                                     cTATATGGATGAACGCTAGGGCGAAAAAATGTTATCGGAAACGTTGCGCCCGAAAGTAGCGGCCGGGTTAAGGCAGGTGGGCAGCTATCAAAATCATCTGGTCACCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGAc                                                                                                                                                                                           <  1:165052/250‑1 (MQ=255)                                       atatTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATAc                                                                                                                                                                                                                          >  1:121174/1‑217 (MQ=255)                                       atatTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTAttt                                                                                                                                                                                                              <  1:350883/229‑1 (MQ=255)                                       atatTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTa                                                                                                                                                                                        >  1:546129/1‑251 (MQ=255)                                       atatTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTa                                                                                                                                                                                        >  1:71018/1‑251 (MQ=255)                                       atatTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTa                                                                                                                                                                                        >  1:406673/1‑251 (MQ=255)                                       atatTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTa                                                                                                                                                                                        >  1:621607/1‑251 (MQ=255)                                       atatTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTa                                                                                                                                                                                        >  1:187749/1‑251 (MQ=255)                                                  cGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAACATCCATCAGTGATTTTGCCGAAAATCTTGCGAATAc                                                                                                                                                                                                                          >  1:452858/1‑206 (MQ=255)                                                                          gAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTg                                                                                                                                                      >  1:81857/1‑250 (MQ=255)                                                                                 agccggcGGAATTAGCAGCCTGTTAAAAGCGGGGCGGCACCTACCAAAATCATATGTTCCCCCCCCTTTTTCCCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGTTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTAATTTTGCCGAAAATCTTGCGAATACCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTaa                                                                                                                                                <  1:788982/243‑1 (MQ=255)                                                                                        ggAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCAACACCTATTTCGCCCGCCTCGCCCCTGACt                                                                                                                                                                                          >  1:432528/1‑200 (MQ=255)                                                                                         gAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGc                                                                                                                                        >  1:651444/1‑249 (MQ=255)                                                                                         gAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGc                                                                                                                                        >  1:202142/1‑249 (MQ=255)                                                                                                cagcCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGTTCACCCCACGCTTTCCCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTtctc                                                                                                                                 <  1:358036/249‑1 (MQ=255)                                                                                                      ggTTAAAGCGGGTCGGCAGCTATCAAAATCATCTGGTCACCCCCCGCTTTCCCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGcaca                                                                                                                                                                          <  1:785545/202‑1 (MQ=255)                                                                                                      ggTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTCGATTATTTCACCGCAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGACAGCCTTTCTCTGGAAGa                                                                                                                          >  1:466103/1‑250 (MQ=255)                                                                                                      ggTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACtt                                                                                                                                                                                         >  1:397779/1‑187 (MQ=255)                                                                                                      ggTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCAAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGa                                                                                                                          >  1:464867/1‑250 (MQ=255)                                                                                                             gCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCagtgtccgactgg                                                                                                                                                                       >  1:785218/1‑186 (MQ=255)                                                                                                             gCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTg                                                                                                                  >  1:109581/1‑251 (MQ=255)                                                                                                                ggTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGcac                                                                                                                                                                           >  1:558945/1‑191 (MQ=255)                                                                                                                ggTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGttt                                                                                                               >  1:531167/1‑251 (MQ=255)                                                                                                                          tATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATc                                                                                                       >  1:668879/1‑249 (MQ=255)                                                                                                                           aTCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATc                                                                                                       >  1:835888/1‑248 (MQ=255)                                                                                                                                  caatcTGGTCTACCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAACCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAAcc                                                                                             <  1:493477/249‑1 (MQ=255)                                                                                                                                        gttcaccccgcgttttACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCAcc                                                                                        <  1:174325/237‑1 (MQ=255)                                                                                                                                            accccACGCTTTCCCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGt                                                                                   <  1:43364/249‑1 (MQ=255)                                                                                                                                             tCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGacac                                                                                                                                                                                                                    <  1:416939/121‑1 (MQ=255)                                                                                                                                             tCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAAc                                                                                                                                              >  1:156010/1‑191 (MQ=255)                                                                                                                                             tCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTa                                                                                  >  1:726729/1‑251 (MQ=255)                                                                                                                                             tCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTa                                                                                  >  1:654217/1‑251 (MQ=255)                                                                                                                                                  cgttttccccGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCAcc                                                                                        <  1:781859/233‑1 (MQ=255)                                                                                                                                                  cGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGCCTTAGTGCAACTTGCACATTTAATCCAGTCGGac                                                                                                                                                          >  1:45291/1‑174 (MQ=255)                                                                                                                                                         cccGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGtt                                                                      >  1:87815/1‑251 (MQ=255)                                                                                                                                                            gCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGc                                                                                                                                                                                                                                            <  1:206724/82‑1 (MQ=255)                                                                                                                                                            gCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCCCTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGtttt                                                                    >  1:437102/1‑250 (MQ=255)                                                                                                                                                            gCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTaa                                                                                                                                                <  1:12120/174‑1 (MQ=255)                                                                                                                                                            gCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATcc                                                                                                      >  1:636353/1‑216 (MQ=255)                                                                                                                                                            gCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGtttt                                                                    >  1:575899/1‑250 (MQ=255)                                                                                                                                                             ccTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGtttcc                                                                   >  1:809456/1‑248 (MQ=255)                                                                                                                                                             ccTAGGGCTGCCTAAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATcc                                                                                                                                                                  <  1:221445/155‑1 (MQ=255)                                                                                                                                                                aGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCccg                                                                >  1:641911/1‑250 (MQ=255)                                                                                                                                                                      gCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGacac                                                                                                                                                        >  1:612220/1‑156 (MQ=255)                                                                                                                                                                          gAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCaa                                                     >  1:338978/1‑251 (MQ=255)                                                                                                                                                                          gAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCaa                                                     >  1:790972/1‑251 (MQ=255)                                                                                                                                                                          gAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCaa                                                     >  1:658903/1‑251 (MQ=255)                                                                                                                                                                                  cACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCAcc                                                                                        <  1:330284/208‑1 (MQ=255)                                                                                                                                                                                   cccaaaaGTGCGGTAGATTATTTCACCAAAAATAAAGAAAAACCCATCAGTGATTTTGCCGAAAATCTTGCGAATCCCGCCCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTAtgt                                            <  1:563536/247‑1 (MQ=255)                                                                                                                                                                                   aCCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTAtgt                                            >  1:478385/1‑251 (MQ=255)                                                                                                                                                                                           tGCGGTAGATTATTCCCCCGAAAAAAAAGAAAAACCCACCAGTGATTTTGCCGAAAATCTGGCGAATACCGACACTTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAAc                                      <  1:613537/249‑1 (MQ=255)                                                                                                                                                                                           ggCGGTAGATTATTCCCCCGAAAAAAAAAAAAAACCCATCAGTAATTTTGCCAAAAATCTTGCAAATCCCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAAc                                      <  1:335059/248‑1 (MQ=255)                                                                                                                                                                                            gCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATc                                                                                                       >  1:443935/1‑183 (MQ=255)                                                                                                                                                                                               gTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAAc                                                                                                                                              >  1:114565/1‑141 (MQ=255)                                                                                                                                                                                                tAGATTATTTCCCCGAAAATAAAAAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTcc                               <  1:865371/251‑1 (MQ=255)                                                                                                                                                                                                   attattTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGt                            >  1:313483/1‑251 (MQ=255)                                                                                                                                                                                                    ttattTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGtt                           >  1:814518/1‑251 (MQ=255)                                                                                                                                                                                                                aaaTAAAAAAAAACCCATCAGTGATTTTGCCAAAATTTTTGCGAATCCCGACCCCTATTCCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGc               <  1:698539/251‑1 (MQ=255)                                                                                                                                                                                                                      aGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTg                                                                                      >  1:514423/1‑174 (MQ=255)                                                                                                                                                                                                                             cccATCAGTGTTTTTGCCGAAAATCTTGCAAATACCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGa  <  1:106372/250‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     CGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCGGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGA  >  1/997150‑997620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑