Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	867,145	C→T	intergenic (‑15947/‑56572)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	867,145	0	C	T	100.0%	253.0 / NA	72	intergenic (‑15947/‑56572)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/45);  total (27/45)

GATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGCTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTAT  >  1/866900‑867385                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 gatgaCATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTcga                                                                                                                                                                                                                                              >  1:792636/1‑250 (MQ=255) gatgaCATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTcga                                                                                                                                                                                                                                              >  1:589438/1‑250 (MQ=255)         tCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATcc                                                                                                                                                                                                                                     <  1:830768/251‑1 (MQ=255)              aTAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAAc                                                                                                                                                                                                                                <  1:653584/251‑1 (MQ=255)                                    ttGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTAGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGagaa                                                                                                                                                                                                          >  1:689557/1‑251 (MQ=255)                                              gAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAAc                                                                                                                                                                                                 <  1:301853/250‑1 (MQ=255)                                              gAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACg                                                                                                                                                                                                >  1:678144/1‑251 (MQ=255)                                                             ttGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATc                                                                                                                                                                                 <  1:283630/251‑1 (MQ=255)                                                             ttGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATc                                                                                                                                                                                 <  1:603870/251‑1 (MQ=255)                                                                       aTTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGcac                                                                                                                                                                       <  1:74588/251‑1 (MQ=255)                                                                          tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATc                                                                                                                                                                                 <  1:456691/238‑1 (MQ=255)                                                                          tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                                                                                    <  1:486776/251‑1 (MQ=255)                                                                          tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                                                                                    <  1:361764/251‑1 (MQ=255)                                                                          tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                                                                                    <  1:519951/251‑1 (MQ=255)                                                                               ttCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGgaga                                                                                                                                                                                   <  1:674734/231‑1 (MQ=255)                                                                                   tATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCAc                                                                                                                                                                                             <  1:517075/217‑1 (MQ=255)                                                                                    aTGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAAc                                                                                                                                                          <  1:124046/251‑1 (MQ=255)                                                                                      gATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                                                                                    >  1:323701/1‑239 (MQ=255)                                                                                      gATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACAt                                                                                                                                                        >  1:644077/1‑251 (MQ=255)                                                                                            aacCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAAc                                                                                                                                                  <  1:338368/251‑1 (MQ=255)                                                                                             acCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGaagaaaga                                                                                                                                                                                                    >  1:465414/1‑200 (MQ=255)                                                                                                cAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTAtt                                                                                                                                              <  1:237814/251‑1 (MQ=255)                                                                                                  aaaTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGaagaaaga                                                                                                                                                                                                    <  1:67888/195‑1 (MQ=255)                                                                                                    aTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTtata                                                                                                                                          <  1:48770/251‑1 (MQ=255)                                                                                                                cGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGaaaaa                                                                                                                              >  1:557630/1‑251 (MQ=255)                                                                                                                   cTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACt                                                                                                                           <  1:243630/251‑1 (MQ=255)                                                                                                                       ggaggCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTgg                                                                                                                        >  1:497088/1‑250 (MQ=255)                                                                                                                        gaggCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGc                                                                                                                       <  1:151698/250‑1 (MQ=255)                                                                                                                          ggCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                                                                                    >  1:75581/1‑203 (MQ=255)                                                                                                                            cAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTccc                                                                                                                   >  1:122096/1‑250 (MQ=255)                                                                                                                             aaaTATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGgag                                                                                                                                                                                    <  1:520510/184‑1 (MQ=255)                                                                                                                                  tGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGaagaaaga                                                                                                                                                                                                    <  1:27244/163‑1 (MQ=255)                                                                                                                                   gACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAAcgg                                                                                                                                                                                                                 <  1:755964/149‑1 (MQ=255)                                                                                                                                            agCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGaa                                                                                                                                                            <  1:20633/193‑1 (MQ=255)                                                                                                                                            agCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAg                                                                                                   >  1:764416/1‑250 (MQ=255)                                                                                                                                                 aaaaaGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGc                                                                                             <  1:212320/251‑1 (MQ=255)                                                                                                                                                          ggttAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACACCTGTTCGAAACATTGTaa                                                                                                                                                   >  1:429203/1‑188 (MQ=255)                                                                                                                                                           gttAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAAc                                                                                                                                                  >  1:169392/1‑188 (MQ=255)                                                                                                                                                           gttAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTAtt                                                                                                                                              <  1:741655/192‑1 (MQ=255)                                                                                                                                                             tAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGt                                                                                 <  1:494128/251‑1 (MQ=255)                                                                                                                                                             tAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGt                                                                                 <  1:590924/251‑1 (MQ=255)                                                                                                                                                             tAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGt                                                                                 <  1:692890/251‑1 (MQ=255)                                                                                                                                                             tAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGt                                                                                 <  1:532025/251‑1 (MQ=255)                                                                                                                                                                    cgcTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                                                                                    <  1:689049/161‑1 (MQ=255)                                                                                                                                                                        tGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACACTGTAACTa                                                                                                                                                >  1:289443/1‑177 (MQ=255)                                                                                                                                                                         gggATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTa                                                                                                                                                <  1:473958/176‑1 (MQ=255)                                                                                                                                                                         gggATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGc                                                                                             <  1:36691/227‑1 (MQ=255)                                                                                                                                                                             tGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGt                                                                                 >  1:329222/1‑235 (MQ=255)                                                                                                                                                                              gCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                                                                                    <  1:454924/151‑1 (MQ=255)                                                                                                                                                                                  aTTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGaagaaaga                                                                                                                                                                                                    >  1:757157/1‑115 (MQ=255)                                                                                                                                                                                    tCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGc                                                          <  1:14479/251‑1 (MQ=255)                                                                                                                                                                                                          gtgtGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                                                                                    >  1:750547/1‑123 (MQ=255)                                                                                                                                                                                                          gtgtGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                                                                                    <  1:391889/123‑1 (MQ=255)                                                                                                                                                                                                          gtgtGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAAc                                                                                                                                                          >  1:47695/1‑133 (MQ=255)                                                                                                                                                                                                          gtgtGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTaa                                                                                                                                                   <  1:680107/140‑1 (MQ=255)                                                                                                                                                                                                          gtgtGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCa                                                                            <  1:605292/211‑1 (MQ=255)                                                                                                                                                                                                          gtgtGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAg                                    >  1:365343/1‑251 (MQ=255)                                                                                                                                                                                                            gtgGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTtatat                                                                                                                                         >  1:69407/1‑148 (MQ=255)                                                                                                                                                                                                             tgGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGAt                                  <  1:570497/250‑1 (MQ=255)                                                                                                                                                                                                               gATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTaa                                                                                                                                                   <  1:625627/135‑1 (MQ=255)                                                                                                                                                                                                               gATCAAGGCACTTGCCGGGGCTGCGTGCCGGCGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGt                                                                                 <  1:441259/201‑1 (MQ=255)                                                                                                                                                                                                                  cAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTa                                                                                                                                                <  1:323358/135‑1 (MQ=255)                                                                                                                                                                                                                         cTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCtt                      >  1:737887/1‑250 (MQ=255)                                                                                                                                                                                                                           tGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCg                   >  1:704721/1‑251 (MQ=255)                                                                                                                                                                                                                             ccGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGcc                 <  1:233468/251‑1 (MQ=255)                                                                                                                                                                                                                              cGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGccc                <  1:526883/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCtt              <  1:454825/250‑1 (MQ=255)                                                                                                                                                                                                                                     gCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAg                                                                                  >  1:370258/1‑178 (MQ=255)                                                                                                                                                                                                                                     gCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCggg          >  1:270103/1‑250 (MQ=255)                                                                                                                                                                                                                                        tGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGACAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGTTCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACg      >  1:424490/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCTGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGt     <  1:452456/250‑1 (MQ=255)                                                                                                                                                                                                                                            ggtggtGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTAt  >  1:837115/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 GATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGCTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTAT  >  1/866900‑867385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑