Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	779,539	T→C	intergenic (+44666/+42294)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	779,539	0	T	C	100.0%	50.6 / NA	16	intergenic (+44666/+42294)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/11);  total (5/11)

AAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGTTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTCTGTACATAACTCATATCCCAGCAAGGAGTACAAATTATGAATATTCATCATATCATTATCTCTATTCTTATCGCAAGACAA‑‑‑‑‑‑‑‑ACCGGTACATATCCCGGCAATGAAAGGTAAAGAATTTGCCGAATTCATGTCATTGTTGCATATTGCGTTAACTCAATCT  >  1/779301‑779765                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           aaacaatacttcttCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCt                                                                                                                                                                                                                                <  1:737277/243‑1 (MQ=255)                                                                  tgcatATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGttggcacataac                                                                                                                                                                <  1:593174/247‑12 (MQ=255)                                                                      tATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACttat                                                                                                                                                            >  1:592425/1‑248 (MQ=255)                                                                                          tCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCGCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACaaa                                                                                                                                        <  1:434772/251‑1 (MQ=255)                                                                                            cTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAAtc                                                                                                                                      <  1:895159/251‑2 (MQ=255)                                                                                                                                 acaacaATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATc                                                                                                  <  1:458486/250‑1 (MQ=255)                                                                                                                                                tattaACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCt                                                                                                                                                                                                                                >  1:858519/1‑109 (MQ=255)                                                                                                                                                     aCATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTc                                                                                                                                                                                       <  1:371622/145‑1 (MQ=255)                                                                                                                                                                       gATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGttggcacataac                                                                                                                                                                <  1:159326/150‑12 (MQ=255)                                                                                                                                                                       gATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAAtc                                                                                                                                      <  1:766106/176‑2 (MQ=255)                                                                                                                                                                                             atGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATCGCAAGCCAA‑‑‑‑‑‑‑‑Actggcagaccggtacac                                                               <  1:370051/217‑17 (MQ=255)                                                                                                                                                                                               gTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATCGc                                                                                                <  1:893741/190‑1 (MQ=255)                                                                                                                                                                                                                        tatttattGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATCGCAAGCCAAACTGGCAGACCGGTACACATTCCGGCGATGAAAGGCAGGGAATTTGCCGAATTCATGTCAttgttacgcattgcattac          <  1:895597/251‑15 (MQ=255)                                                                                                                                                                                                                          tttattGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTAtctc                                                                                                            >  1:447812/1‑151 (MQ=255)                                                                                                                                                                                                                                gATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATCGCAAGCCAAACTGGCAGACCGGTACACATTCCGGCGATGAAAGGCAGGGAATTTGCCGAATTCATGTCAttgttacgcattgcattacagtcttaa  >  1:73272/1‑229 (MQ=255)                                                                                                                                                                                                                                 aTTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCag                                                                                                                                                                               >  1:813876/1‑77 (MQ=255)                                                                                                                                                                                                                                       gtttttatgctttGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTc                                                                                                       <  1:107181/143‑1 (MQ=255)                                                                                                                                                                                                                                        tttttatgctttGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGAt                                                                                                                                                                                              >  1:618992/1‑55 (MQ=255)                                                                                                                                                                                                                                        tttttatgctttGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATgcaacttaatca                                                                                                                        <  1:525371/125‑12 (MQ=255)                                                                                                                                                                                                                                          tttatgctttGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACaa                                                                                                                                         >  1:295821/1‑106 (MQ=255)                                                                                                                                                                                                                                          tttatgctttGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATCGCAAGCCAAACTGGCAGACCGGTACACATTCCGGCGATGAAAGGCAGGGAATTTGCCGAATTCATGTCAttgttacgcattgcattac          >  1:516886/1‑219 (MQ=255)                                                                                                                                                                                                                                          tttatgctttGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATCGCAAGCCAAACTGGCAGACCGGTACACATTCCGGCGATGAAAGGCAGGGAATTTGCCGAATTCATGTCAttgttacgcattgcattac          <  1:629046/233‑15 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           AAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGTTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTCTGTACATAACTCATATCCCAGCAAGGAGTACAAATTATGAATATTCATCATATCATTATCTCTATTCTTATCGCAAGACAA‑‑‑‑‑‑‑‑ACCGGTACATATCCCGGCAATGAAAGGTAAAGAATTTGCCGAATTCATGTCATTGTTGCATATTGCGTTAACTCAATCT  >  1/779301‑779765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑