Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	779,499	T→C	intergenic (+44626/+42334)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	779,499	0	T	C	100.0%	38.0 / NA	13	intergenic (+44626/+42334)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/10);  total (3/10)

AAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGTTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTCTGTACATAACTCATATCCCAGCAAGGAGTACAAATTATGAATATTCATCATATCATTATCTCTATTCTTATCGCAAGACAAACCGGTACATATCCCGGC  >  1/779301‑779704                                                                                                                                                                                                        |                                                                                                                                                                                                              aaacaatacttcttCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCt                                                                                                                                                           <  1:737277/243‑1 (MQ=255)                                                                cttgcatATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAata                                                                                                                                                                                                        >  1:731009/7‑144 (MQ=255)                                                                  tgcatATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGttggcacataac                                                                                           <  1:593174/247‑12 (MQ=255)                                                                      tATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACttat                                                                                       >  1:592425/1‑248 (MQ=255)                                                                                          tCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCGCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACaaa                                                                   <  1:434772/251‑1 (MQ=255)                                                                                            cTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAAtc                                                                 <  1:895159/251‑2 (MQ=255)                                                                                                                                 acaacaATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATc                             <  1:458486/250‑1 (MQ=255)                                                                                                                                                tattaACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCt                                                                                                                                                           >  1:858519/1‑109 (MQ=255)                                                                                                                                                     aCATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTc                                                                                                                  <  1:371622/145‑1 (MQ=255)                                                                                                                                                                       gATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGttggcacataac                                                                                           <  1:159326/150‑12 (MQ=255)                                                                                                                                                                       gATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAAtc                                                                 <  1:766106/176‑2 (MQ=255)                                                                                                                                                                                             atGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATCGCAAGCCAAActggcagaccggtacac  <  1:370051/217‑17 (MQ=255)                                                                                                                                                                                               gTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATCGc                           <  1:893741/190‑1 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                              AAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGTTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTCTGTACATAACTCATATCCCAGCAAGGAGTACAAATTATGAATATTCATCATATCATTATCTCTATTCTTATCGCAAGACAAACCGGTACATATCCCGGC  >  1/779301‑779704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑