Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	779,472	A→G	intergenic (+44599/+42361)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	779,472	0	A	G	100.0%	40.5 / NA	13	intergenic (+44599/+42361)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/9);  total (4/9)

AGGTTAGCTAATACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGTTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTCTGTACATAACTCATATCCCAGCAAGGAGTACAAATTATGAATATTCATCATATCATTATCTCTATTCTTATC  >  1/779239‑779677                                                                                                                                                                                                                                           |                                                                                                                                                                                                              aGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATaaa                                                                                                                                                                                                       <  1:654542/242‑1 (MQ=255)            caCTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGCTAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTAtgcg                                                                                                                                                                                   >  1:441258/2‑249 (MQ=255)                                                               aaacaatacttcttCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCt                                                                                                                                <  1:737277/243‑1 (MQ=255)                                                                                                                              cttgcatATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAata                                                                                                                                                                             >  1:731009/7‑144 (MQ=255)                                                                                                                                tgcatATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGttggcacataac                                                                <  1:593174/247‑12 (MQ=255)                                                                                                                                    tATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACttat                                                            >  1:592425/1‑248 (MQ=255)                                                                                                                                                        tCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCGCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACaaa                                        <  1:434772/251‑1 (MQ=255)                                                                                                                                                          cTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAAtc                                      <  1:895159/251‑2 (MQ=255)                                                                                                                                                                                               acaacaATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATc  <  1:458486/250‑1 (MQ=255)                                                                                                                                                                                                              tattaACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCt                                                                                                                                >  1:858519/1‑109 (MQ=255)                                                                                                                                                                                                                   aCATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTc                                                                                       <  1:371622/145‑1 (MQ=255)                                                                                                                                                                                                                                     gATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGttggcacataac                                                                <  1:159326/150‑12 (MQ=255)                                                                                                                                                                                                                                     gATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAAtc                                      <  1:766106/176‑2 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                              AGGTTAGCTAATACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGTTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTCTGTACATAACTCATATCCCAGCAAGGAGTACAAATTATGAATATTCATCATATCATTATCTCTATTCTTATC  >  1/779239‑779677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑