Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	779,211	A→G	intergenic (+44338/+42622)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	779,211	0	A	G	100.0%	177.0 / NA	52	intergenic (+44338/+42622)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/27);  total (25/27)

ATATAAACCCTGCCATTCCGCACAATAAATTTTACGCTATGCCCGTTTTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAGCGAAAAGAAACTTCCCTGCTTTGACTATCATACAAAATAAAACGAGCTTTTTCTAAAAGCTGTTGTTCCGCTTCTTCCATCCAAACTACGGTGTAATCCAATTTCATTTTAAGCCCAATACTTTTTCCAGGTTAGCTAATACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATA  >  1/778973‑779456                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       atatAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTGTAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAg                                                                                                                                                                                                                                            >  1:529975/1‑251 (MQ=255) atatAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAg                                                                                                                                                                                                                                            >  1:118251/1‑251 (MQ=255) atatAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAg                                                                                                                                                                                                                                            >  1:540683/1‑251 (MQ=255) atatAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAg                                                                                                                                                                                                                                            >  1:880406/1‑251 (MQ=255)  tataAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAgt                                                                                                                                                                                                                                           >  1:572458/1‑250 (MQ=255)       aCCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAAt                                                                                                                                                                                                                                      <  1:544835/251‑1 (MQ=255)         ccTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATAc                                                                                                                                                                                                                                    <  1:635224/251‑1 (MQ=255)          ctgtcATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATa                                                                                                                                                                                                                                     >  1:751239/5‑249 (MQ=255)          ctgtcATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATa                                                                                                                                                                                                                                     >  1:310625/5‑249 (MQ=255)                          tAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTa                                                                                                                                                                                                                   <  1:499693/251‑1 (MQ=255)                              ttttACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTaacac                                                                                                                                                                                                               <  1:357153/251‑4 (MQ=255)                                   cGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTcc                                                                                                                                                                                                                             <  1:880829/232‑1 (MQ=255)                                              ttcttAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGAttta                                                                                                                                                                                               >  1:172116/4‑250 (MQ=255)                                                         ataGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTtc                                                                                                                                                                                    <  1:647162/251‑1 (MQ=255)                                                            gATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTtctc                                                                                                                                                                                  >  1:494770/1‑250 (MQ=255)                                                             atatGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACttcttc                                                                                                                                                                                                         <  1:584562/226‑1 (MQ=255)                                                             atatGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCg                                                                                                                                                                                >  1:623180/1‑251 (MQ=255)                                                                     tCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCt                                                                                                                                                                                                                    <  1:668694/207‑1 (MQ=255)                                                                      cGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTaac                                                                                                                                                                       <  1:96777/251‑3 (MQ=255)                                                                             catTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGtt                                                                                                                                                                                                                        <  1:162446/195‑1 (MQ=255)                                                                              atTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                                                                                                                                                     >  1:500174/1‑245 (MQ=255)                                                                              atTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                                                                                                                                                     >  1:362266/1‑245 (MQ=255)                                                                              atTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaa                                                                                                                                                               >  1:313485/1‑251 (MQ=255)                                                                               ttATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                                                                                                                                                     <  1:392137/244‑1 (MQ=255)                                                                                        gTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTaacac                                                                                                                                                                                                               <  1:198665/193‑4 (MQ=255)                                                                                        gTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaaaaacaatac                                                                                                                                                      <  1:506439/250‑7 (MQ=255)                                                                                        gTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaaaaacaatac                                                                                                                                                      <  1:727580/250‑7 (MQ=255)                                                                                            cTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGAt                                                                                                                                                                                                  <  1:275858/202‑1 (MQ=255)                                                                                            cTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTaac                                                                                                                                                                       <  1:630338/229‑3 (MQ=255)                                                                                            cTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaaaaacaatacttctt                                                                                                                                                 >  1:897615/1‑240 (MQ=255)                                                                                              aCATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATAc                                                                                                                                                                                                                                    >  1:268657/1‑166 (MQ=255)                                                                                              aCATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTaac                                                                                                                                                                       <  1:545803/227‑3 (MQ=255)                                                                                              aCATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                                                                                                                                                     <  1:179950/229‑1 (MQ=255)                                                                                                aTAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTaac                                                                                                                                                                                                                 >  1:494815/1‑182 (MQ=255)                                                                                                aTAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTaac                                                                                                                                                                       >  1:198054/1‑223 (MQ=255)                                                                                                aTAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                                                                                                                                                     <  1:734928/227‑1 (MQ=255)                                                                                                 tAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGaa                                                                                                                                            <  1:726771/251‑1 (MQ=255)                                                                                                   ggATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAg                                                                                                                                          >  1:413180/1‑251 (MQ=255)                                                                                                   ggATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAg                                                                                                                                          >  1:715473/1‑251 (MQ=255)                                                                                                        gTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTaacc                                                                                                                                                                      <  1:522284/218‑4 (MQ=255)                                                                                                           tttGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACtt                                                                                                                                  <  1:699369/251‑1 (MQ=255)                                                                                                               ggTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGtt                                                                                                                                                                                                                        >  1:496670/1‑161 (MQ=255)                                                                                                                                                                                                tAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGGCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATc                                            <  1:277438/251‑1 (MQ=255)                                                                                                                                                                                                      ctgctgctCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCa                                                 >  1:473074/8‑240 (MQ=255)                                                                                                                                                                                                             tCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCg                               <  1:859939/251‑1 (MQ=255)                                                                                                                                                                                                               cGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATc                                            >  1:889129/1‑236 (MQ=255)                                                                                                                                                                                                                       ccgtccaAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAg                     <  1:500328/248‑1 (MQ=255)                                                                                                                                                                                                                          tccaAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCaa                  <  1:716382/251‑1 (MQ=255)                                                                                                                                                                                                                                     gtgtAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGAt                                      >  1:624756/1‑220 (MQ=255)                                                                                                                                                                                                                                     gtgtAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAAc         >  1:69249/1‑249 (MQ=255)                                                                                                                                                                                                                                     gtgtAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACAt       >  1:284637/1‑251 (MQ=255)                                                                                                                                                                                                                                          aTCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAata  <  1:94911/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       ATATAAACCCTGCCATTCCGCACAATAAATTTTACGCTATGCCCGTTTTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAGCGAAAAGAAACTTCCCTGCTTTGACTATCATACAAAATAAAACGAGCTTTTTCTAAAAGCTGTTGTTCCGCTTCTTCCATCCAAACTACGGTGTAATCCAATTTCATTTTAAGCCCAATACTTTTTCCAGGTTAGCTAATACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATA  >  1/778973‑779456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑