Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	778,929	A→C	intergenic (+44056/+42904)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	778,929	0	A	C	100.0%	122.2 / NA	37	intergenic (+44056/+42904)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/17);  total (20/17)

AATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAACTTTTTAAAACTATGCCCGGATGCTAGTGATGATTCAGTGAACCAGGCTTTATCTACAAAATGCGCCCGCAAGCCGTTCAGTCGAAAATGTATATTTAAATAAAAACATAAACACATATAAAAATAAAAGCATAAATTGATATTTATGCTTTTATTG‑TTTTTGAGGTGATATAAAGCAACGGCTATATTACAAATTCACGCCCTTTTGCCAAGAAAGCGGAAATATAAACCCTGCCATTCCGCACAATAAATTTTACGCTATGCCCGTTTTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAGCGAAAAGAAACTTCCCTGCTTTGACTATCATACAAAATAAAACGAGCTTTTTCTAAAAG  >  1/778692‑779169                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 aaTAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAATTTTTTAAAATTGTGTCCAAATTCTAACGAGGACTCTATTAATCAAGCATTATCGAGCAAATGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTa                                                                                                                                                                                                                                       >  1:603365/1‑250 (MQ=255)           aTTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAATTTTTTAAAATTGTGTCCAAATTCTAACGAGGACTCTATTAATCAAGCATTATCGAGCAAATGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTcccg                                                                                                                                                                                                                             >  1:724935/1‑247 (MQ=255)                     ggCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAATTTTTTAAAATTGTGTCCAAATTCTAACGAGGACTCTATTAATCAAGCATTATCGAGCAAATGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGcta                                                                                                                                                                                                                  >  1:17085/1‑249 (MQ=255)                                                                                                             ttatcgagcaaaTGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACcat                                                                                                                           >  1:33572/10‑250 (MQ=255)                                                                                                                        aTGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCgg                                                                                                               <  1:867792/251‑1 (MQ=255)                                                                                                                                      cGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGAt                                                                                                 <  1:744425/251‑1 (MQ=255)                                                                                                                                                  gatgtatgtTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGaaa                                                                                                                                   <  1:817033/204‑1 (MQ=255)                                                                                                                                                            taaataaaAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTaa                                                                           <  1:685692/251‑1 (MQ=255)                                                                                                                                                                 aaaaaCATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTcc                                                                      <  1:179223/251‑1 (MQ=255)                                                                                                                                                                                     gaataaaaGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAAt                                                                                                                                  <  1:588393/170‑1 (MQ=255)                                                                                                                                                                                        taaaaGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACACACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGaaattt                                                >  1:224829/1‑247 (MQ=255)                                                                                                                                                                                                        tatTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACAt                                                                                                      <  1:865165/180‑1 (MQ=255)                                                                                                                                                                                                                cTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAAt                                                                          >  1:878131/1‑200 (MQ=255)                                                                                                                                                                                                                cTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTcc                                                                      <  1:525200/204‑1 (MQ=255)                                                                                                                                                                                                                    tATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATaaag                   <  1:780633/251‑2 (MQ=255)                                                                                                                                                                                                                          tttttGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTggg                                                                                       <  1:692009/177‑1 (MQ=255)                                                                                                                                                                                                                          tttttGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACaa                          <  1:861679/238‑1 (MQ=255)                                                                                                                                                                                                                          tttttGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAg               >  1:565232/1‑249 (MQ=255)                                                                                                                                                                                                                                aGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCttttt         >  1:36548/1‑249 (MQ=255)                                                                                                                                                                                                                                  gTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTaa                                                                           <  1:343508/181‑1 (MQ=255)                                                                                                                                                                                                                                  gTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACt                                   >  1:497216/1‑221 (MQ=255)                                                                                                                                                                                                                                    gATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACaa                          >  1:817324/1‑228 (MQ=255)                                                                                                                                                                                                                                    gATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTaaaa   >  1:552574/1‑251 (MQ=255)                                                                                                                                                                                                                                    gATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTaaaa   >  1:391340/1‑251 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACcatcat                                                                                                                        <  1:441804/133‑1 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAg                                                                                                                  >  1:682308/1‑139 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACAt                                                                                                      <  1:643197/151‑1 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGttt                                                                                            <  1:460038/161‑1 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTaa                                                                           >  1:408357/1‑178 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAAttt                                                                        >  1:71607/1‑181 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGAc                                    <  1:535816/217‑1 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAAt                       <  1:87948/230‑1 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAg  >  1:124670/1‑251 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAg  >  1:682644/1‑251 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAg  >  1:270422/1‑251 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAg  >  1:259673/1‑251 (MQ=255)                                                                                                                                                                                                                                     atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAg  >  1:246755/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 AATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAACTTTTTAAAACTATGCCCGGATGCTAGTGATGATTCAGTGAACCAGGCTTTATCTACAAAATGCGCCCGCAAGCCGTTCAGTCGAAAATGTATATTTAAATAAAAACATAAACACATATAAAAATAAAAGCATAAATTGATATTTATGCTTTTATTG‑TTTTTGAGGTGATATAAAGCAACGGCTATATTACAAATTCACGCCCTTTTGCCAAGAAAGCGGAAATATAAACCCTGCCATTCCGCACAATAAATTTTACGCTATGCCCGTTTTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAGCGAAAAGAAACTTCCCTGCTTTGACTATCATACAAAATAAAACGAGCTTTTTCTAAAAG  >  1/778692‑779169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑