Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	778,898	G→A	intergenic (+44025/+42935)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	778,898	0	G	A	100.0%	43.2 / NA	14	intergenic (+44025/+42935)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/8);  total (6/8)

ATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAACTTTTTAAAACTATGCCCGGATGCTAGTGATGATTCAGTGAACCAGGCTTTATCTACAAAATGCGCCCGCAAGCCGTTCAGTCGAAAATGTATATTTAAATAAAAACATAAACACATATAAAAATAAAAGCATAAATTGATATTTATGCTTTTATTG‑TTTTTGAGGTGATATAAAGCAACGGCTATATTACAAATTCACGCCCTTTTGCCAAGAAAGCGGAAATATAAACCCTGCCATTCCGCACAATAAATTTTACGCTATGCCCGTTTTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAGCGAAAAGAAACTT  >  1/778681‑779123                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   aTCCGCCGTTAAATAAGGTCTATTCAAATCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAATTTTTTAAAATTGTGTCCAAATTCTAACGAGGACTCTATTAATCAAGCATTATCGAGCAAATGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCacc                                                                                                                                                                                                   <  1:357263/251‑3 (MQ=255)            aaTAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAATTTTTTAAAATTGTGTCCAAATTCTAACGAGGACTCTATTAATCAAGCATTATCGAGCAAATGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTa                                                                                                                                                                                         >  1:603365/1‑250 (MQ=255)             aTAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAATTTTTTAAAATTGTGTCCAAATTCTAACGAGGACTCTATTAATCAAGCATTATCGAGCAAATGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATaa                                                                                                                                                                                                         >  1:344237/1‑233 (MQ=255)                      aTTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAATTTTTTAAAATTGTGTCCAAATTCTAACGAGGACTCTATTAATCAAGCATTATCGAGCAAATGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTcccg                                                                                                                                                                               >  1:724935/1‑247 (MQ=255)                                ggCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAATTTTTTAAAATTGTGTCCAAATTCTAACGAGGACTCTATTAATCAAGCATTATCGAGCAAATGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGcta                                                                                                                                                                    >  1:17085/1‑249 (MQ=255)                                                                                                                        ttatcgagcaaaTGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACcat                                                                             >  1:33572/10‑250 (MQ=255)                                                                                                                                   aTGCGCCCGCAAGCCGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCgg                                                                 <  1:867792/251‑1 (MQ=255)                                                                                                                                                 cGTTTAGTCGAAGATGTATGTTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGAt                                                   <  1:744425/251‑1 (MQ=255)                                                                                                                                                             gatgtatgtTTAAATAAAAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGaaa                                                                                     <  1:817033/204‑1 (MQ=255)                                                                                                                                                                       taaataaaAACATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTaa                             <  1:685692/251‑1 (MQ=255)                                                                                                                                                                            aaaaaCATAAATATACTTAAGAATAAAAGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTcc                        <  1:179223/251‑1 (MQ=255)                                                                                                                                                                                                gaataaaaGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAAt                                                                                    <  1:588393/170‑1 (MQ=255)                                                                                                                                                                                                   taaaaGCATAAATATTTATTTATACTTTTATTGTTTTTTGAGGTGATATAACACACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGaaattt  >  1:224829/1‑247 (MQ=255)                                                                                                                                                                                                                   tatTTATACTTTTATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACAt                                                        <  1:865165/180‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   ATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAACTTTTTAAAACTATGCCCGGATGCTAGTGATGATTCAGTGAACCAGGCTTTATCTACAAAATGCGCCCGCAAGCCGTTCAGTCGAAAATGTATATTTAAATAAAAACATAAACACATATAAAAATAAAAGCATAAATTGATATTTATGCTTTTATTG‑TTTTTGAGGTGATATAAAGCAACGGCTATATTACAAATTCACGCCCTTTTGCCAAGAAAGCGGAAATATAAACCCTGCCATTCCGCACAATAAATTTTACGCTATGCCCGTTTTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAGCGAAAAGAAACTT  >  1/778681‑779123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑