Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	778,559	T→C	intergenic (+43686/+43274)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	778,559	0	T	C	100.0%	144.6 / NA	44	intergenic (+43686/+43274)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/21);  total (23/21)

AAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGTGCCAATATCAGTACATATCAGAAAGACCTATTAAAAAATAATGTCAATATAACTTTCAGGAAAGGAGTTAAAAATGAAAAAGCCCTTTAAAATTGGTGTAATCTTTCTTGCTTTATGCAGTATGAGCCAAATAAGCCATGCGGTGGATGAATTAACCGGTGATACCAAAACCGCCTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAACTTTTTAAAAC  >  1/778315‑778761                                                                                                                                                                                                                                                     |                                                                                                                                                                                                           aaGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATg                                                                                                                                                                                                       >  1:416563/1‑250 (MQ=255) aaGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGc                                                                                                                                                                                                      <  1:543900/251‑1 (MQ=255)      aTTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTAt                                                                                                                                                                                                 <  1:792673/251‑1 (MQ=255)            tgaatgaaACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                                                                                            <  1:587181/250‑1 (MQ=255)                  aaaCAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaa                                                                                                                                                                                      <  1:575032/250‑6 (MQ=255)                       gAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaagtcatg                                                                                                                                                                                >  1:104668/1‑240 (MQ=255)                        aaaaaGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGAGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaagtca                                                                                                                                                                                  <  1:901850/248‑10 (MQ=255)                                     tttCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTa                                                                                                                                                                  >  1:132505/1‑251 (MQ=255)                                          aCTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCgg                                                                                                                                                             >  1:850524/1‑251 (MQ=255)                                               gcgcATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATa                                                                                                                                                        >  1:179385/1‑251 (MQ=255)                                                       tGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                                                                                            >  1:138341/1‑207 (MQ=255)                                                           gATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGctt                                                                                                                                            >  1:416471/1‑249 (MQ=255)                                                               gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                                                                                            <  1:103457/199‑1 (MQ=255)                                                               gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggta                                                                                                                                                                                       >  1:596469/1‑200 (MQ=255)                                                               gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGc                                                                                                                                                                               >  1:671611/1‑212 (MQ=255)                                                               gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGa                                                                                                                                        >  1:314977/1‑251 (MQ=255)                                                               gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGa                                                                                                                                        >  1:210422/1‑251 (MQ=255)                                                                      aaTCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATAcga                                                                                                                                                     >  1:26376/1‑229 (MQ=255)                                                                       aTCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTAc                                                                                                                                <  1:30571/251‑1 (MQ=255)                                                                                   ccGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGGTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCACTTGTGAAGCTCTACTCTGCTTatcat                                                                                                                    <  1:442918/251‑1 (MQ=255)                                                                                             ccGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTatca                                                                                                                     <  1:894063/240‑1 (MQ=255)                                                                                                  gCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACgcgg                                                                                                     >  1:825176/1‑251 (MQ=255)                                                                                                     tataAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTAc                                                                                                                                <  1:88811/221‑1 (MQ=255)                                                                                                               ggTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGc                                                                                                                                                                                                      <  1:239120/141‑1 (MQ=255)                                                                                                                       gCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCAt                                                                                 <  1:168404/250‑1 (MQ=255)                                                                                                                            tGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATccgccg                                                                           >  1:338557/1‑251 (MQ=255)                                                                                                                              gggTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATg                                                                                                                                                                       <  1:373705/157‑1 (MQ=255)                                                                                                                              gggTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATg                                                                                                                                                                       <  1:810093/157‑1 (MQ=255)                                                                                                                                           catcagcacacacccaaaaGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGc                                                                                                                           >  1:399862/17‑188 (MQ=255)                                                                                                                                           catcagcacacacccaaaaGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTc                                                               >  1:127959/17‑248 (MQ=255)                                                                                                                                                       cccaaaaGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTActc                                                                                                                              >  1:888267/5‑173 (MQ=255)                                                                                                                                                       cccaaaaGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGAc                                                                                                         <  1:638895/190‑1 (MQ=255)                                                                                                                                                                   tttAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATG‑AAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACg                                   <  1:615130/250‑1 (MQ=255)                                                                                                                                                                      aaaaaaTAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACAAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGaa                                 <  1:654238/251‑1 (MQ=255)                                                                                                                                                                             aatGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTaa                          <  1:545701/251‑1 (MQ=255)                                                                                                                                                                              atGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTaaa                         >  1:638875/1‑251 (MQ=255)                                                                                                                                                                                gttaaTATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGa                       >  1:19413/4‑251 (MQ=255)                                                                                                                                                                                 taaaTATAACTTTCAGAAGAGGAGTGAAAAATAAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTACCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATAAATTACCCGGTGATACAAAAACCGCTTGTAAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGAAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGa                       <  1:706607/248‑1 (MQ=255)                                                                                                                                                                                         aCTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAAt                                                       >  1:287343/1‑210 (MQ=255)                                                                                                                                                                                         aCTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGAATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAAt                                                       >  1:200640/1‑210 (MQ=255)                                                                                                                                                                                                gaagaGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAAtttttt       >  1:745600/5‑251 (MQ=255)                                                                                                                                                                                                                          cTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATccgc                                                                             <  1:821279/155‑1 (MQ=255)                                                                                                                                                                                                                                         gatatgtcttGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGa                       >  1:333823/7‑194 (MQ=255)                                                                                                                                                                                                                                              gtcttGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAATTTTTTaaaat  <  1:699793/209‑2 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                           AAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGTGCCAATATCAGTACATATCAGAAAGACCTATTAAAAAATAATGTCAATATAACTTTCAGGAAAGGAGTTAAAAATGAAAAAGCCCTTTAAAATTGGTGTAATCTTTCTTGCTTTATGCAGTATGAGCCAAATAAGCCATGCGGTGGATGAATTAACCGGTGATACCAAAACCGCCTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGAAACGTCGTAACTTTTTAAAAC  >  1/778315‑778761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑