Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	778,509	A→G	intergenic (+43636/+43324)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	778,509	0	A	G	100.0%	168.6 / NA	49	intergenic (+43636/+43324)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/20);  total (29/20)

ATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGTGCCAATATCAGTACATATCAGAAAGACCTATTAAAAAATAATGTCAATATAACTTTCAGGAAAGGAGTTAAAAATGAAAAAGCCCTTTAAAATTGGTGTAATCTTTCTTGCTTTATGCAGTATGAGCCAAATAAGCCATGCGGTGGATGAATTAACCGGTGATACCAAAACCGCCTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGA  >  1/778269‑778740                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        aTCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTaaa                                                                                                                                                                                                                               >  1:500486/1‑251 (MQ=255)              aCCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGcc                                                                                                                                                                                                                   >  1:636335/1‑250 (MQ=255)              aCCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGcc                                                                                                                                                                                                                   >  1:513522/1‑250 (MQ=255)                     gTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTaaa                                                                                                                                                                                                            >  1:477520/1‑251 (MQ=255)                                tgtgAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgata                                                                                                                                                                                                >  1:145910/1‑241 (MQ=255)                                tgtgAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgata                                                                                                                                                                                                >  1:156918/1‑241 (MQ=255)                                     aCCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgatatgt                                                                                                                                                                                             >  1:6629/1‑236 (MQ=255)                                       cTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgatatgtcttg                                                                                                                                                                                         >  1:354759/1‑234 (MQ=255)                                           gTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgatatgtcttgcct                                                                                                                                                                                      <  1:674266/250‑21 (MQ=255)                                               aaGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATg                                                                                                                                                                                  >  1:416563/1‑250 (MQ=255)                                               aaGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGc                                                                                                                                                                                 <  1:543900/251‑1 (MQ=255)                                                    aTTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTAt                                                                                                                                                                            <  1:792673/251‑1 (MQ=255)                                                          tgaatgaaACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                                                                       <  1:587181/250‑1 (MQ=255)                                                                aaaCAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaa                                                                                                                                                                 <  1:575032/250‑6 (MQ=255)                                                                     gAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaagtcatg                                                                                                                                                           >  1:104668/1‑240 (MQ=255)                                                                      aaaaaGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGAGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaagtca                                                                                                                                                             <  1:901850/248‑10 (MQ=255)                                                                                    tttCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTa                                                                                                                                             >  1:132505/1‑251 (MQ=255)                                                                                         aCTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCgg                                                                                                                                        >  1:850524/1‑251 (MQ=255)                                                                                              gcgcATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATa                                                                                                                                   >  1:179385/1‑251 (MQ=255)                                                                                                      tGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                                                                       >  1:138341/1‑207 (MQ=255)                                                                                                          gATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGctt                                                                                                                       >  1:416471/1‑249 (MQ=255)                                                                                                              gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                                                                       <  1:103457/199‑1 (MQ=255)                                                                                                              gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggta                                                                                                                                                                  >  1:596469/1‑200 (MQ=255)                                                                                                              gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGc                                                                                                                                                          >  1:671611/1‑212 (MQ=255)                                                                                                              gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGa                                                                                                                   >  1:314977/1‑251 (MQ=255)                                                                                                              gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGa                                                                                                                   >  1:210422/1‑251 (MQ=255)                                                                                                                     aaTCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATAcga                                                                                                                                >  1:26376/1‑229 (MQ=255)                                                                                                                      aTCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTAc                                                                                                           <  1:30571/251‑1 (MQ=255)                                                                                                                                  ccGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGGTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCACTTGTGAAGCTCTACTCTGCTTatcat                                                                                               <  1:442918/251‑1 (MQ=255)                                                                                                                                            ccGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTatca                                                                                                <  1:894063/240‑1 (MQ=255)                                                                                                                                                 gCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACgcgg                                                                                >  1:825176/1‑251 (MQ=255)                                                                                                                                                    tataAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTAc                                                                                                           <  1:88811/221‑1 (MQ=255)                                                                                                                                                              ggTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGc                                                                                                                                                                                 <  1:239120/141‑1 (MQ=255)                                                                                                                                                                      gCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCAt                                                            <  1:168404/250‑1 (MQ=255)                                                                                                                                                                           tGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATccgccg                                                      >  1:338557/1‑251 (MQ=255)                                                                                                                                                                             gggTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATg                                                                                                                                                  <  1:810093/157‑1 (MQ=255)                                                                                                                                                                             gggTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATg                                                                                                                                                  <  1:373705/157‑1 (MQ=255)                                                                                                                                                                                          catcagcacacacccaaaaGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGc                                                                                                      >  1:399862/17‑188 (MQ=255)                                                                                                                                                                                          catcagcacacacccaaaaGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTc                                          >  1:127959/17‑248 (MQ=255)                                                                                                                                                                                                      cccaaaaGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTActc                                                                                                         >  1:888267/5‑173 (MQ=255)                                                                                                                                                                                                      cccaaaaGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGAc                                                                                    <  1:638895/190‑1 (MQ=255)                                                                                                                                                                                                                  tttAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATG‑AAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACg              <  1:615130/250‑1 (MQ=255)                                                                                                                                                                                                                     aaaaaaTAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACAAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGaa            <  1:654238/251‑1 (MQ=255)                                                                                                                                                                                                                            aatGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTaa     <  1:545701/251‑1 (MQ=255)                                                                                                                                                                                                                             atGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTaaa    >  1:638875/1‑251 (MQ=255)                                                                                                                                                                                                                               gttaaTATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGa  >  1:19413/4‑251 (MQ=255)                                                                                                                                                                                                                                taaaTATAACTTTCAGAAGAGGAGTGAAAAATAAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTACCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATAAATTACCCGGTGATACAAAAACCGCTTGTAAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGAAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGa  <  1:706607/248‑1 (MQ=255)                                                                                                                                                                                                                                        aCTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAAt                                  >  1:287343/1‑210 (MQ=255)                                                                                                                                                                                                                                        aCTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGAATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAAt                                  >  1:200640/1‑210 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        ATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGTGCCAATATCAGTACATATCAGAAAGACCTATTAAAAAATAATGTCAATATAACTTTCAGGAAAGGAGTTAAAAATGAAAAAGCCCTTTAAAATTGGTGTAATCTTTCTTGCTTTATGCAGTATGAGCCAAATAAGCCATGCGGTGGATGAATTAACCGGTGATACCAAAACCGCCTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCGTTAAATAAGTTCTATTCAATTCGGGCTAAAAAACCACACGAAACATTAAAGA  >  1/778269‑778740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑