Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	777,535	G→T	intergenic (+42662/+44298)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	777,535	0	G	T	100.0%	244.8 / NA	71	intergenic (+42662/+44298)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/42);  total (29/42)

AACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATTTTGATTTACGCGCCTTGCGCCGGGAAAAAATCACGATTTATTTCCACATTTCAGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCGGCATTAAAATATCAGTTATTACTACTCATGGATGAATTTACTGCGGTCGGGATGTTGAGCATTATCAATAAATCAGTAGGATTTATTGCCGGTTATGGTTTACGCCTATTGATTATCTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCCCCCGACAATCAGGAGGACGCGGAAGAATATTCTAAGGTGCTAGGTAATCGCACTGTAAATAAATTAA  >  1/777289‑777770                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            aaCCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGt                                                                                                                                                                                                                                         <  1:570178/251‑1 (MQ=255)   ccATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTg                                                                                                                                                                                                                                       <  1:518562/251‑1 (MQ=255)   ccATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTg                                                                                                                                                                                                                                       >  1:285079/1‑251 (MQ=255)      tttttCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGc                                                                                                                                                                                                                                    <  1:509584/251‑1 (MQ=255)        tttCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCAt                                                                                                                                                                                                                                  <  1:632859/251‑1 (MQ=255)           cAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTAt                                                                                                                                                                                                                               <  1:608420/251‑1 (MQ=255)                tGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATa                                                                                                                                                                                                                          >  1:786649/1‑251 (MQ=255)                       gttgAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCAt                                                                                                                                                                                                                                  <  1:598106/236‑1 (MQ=255)                           aataTGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAgg                                                                                                                                                                                                                <  1:135605/247‑1 (MQ=255)                                   aCTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTAtcgc                                                                                                                                                                                                       <  1:48175/251‑4 (MQ=255)                                      tCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCgg                                                                                                                                                                                                    >  1:844166/1‑251 (MQ=255)                                       cTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGt                                                                                                                                                                                                   >  1:752925/1‑251 (MQ=255)                                                         tacgcgctttacgcaaggAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTg                                                                                                                                                                                 <  1:112942/235‑1 (MQ=255)                                                              gctttacgcaaggAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATtat                                                                                                                                                                            >  1:534837/12‑251 (MQ=255)                                                                          gAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAAt                                                                                                                                                                                                                           <  1:313429/192‑1 (MQ=255)                                                                          gAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATcaca                                                                                                                                                                >  1:569374/1‑251 (MQ=255)                                                                             aaaaTCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAGTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAgg                                                                                                                                                             <  1:156221/251‑1 (MQ=255)                                                                                  cACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAAc                                                                                                                                                        <  1:22072/251‑1 (MQ=255)                                                                                    cGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACtt                                                                                                                                                      >  1:261/1‑251 (MQ=255)                                                                                    cGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACtt                                                                                                                                                      <  1:833786/251‑1 (MQ=255)                                                                                      atttatttCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCg                                                                                                                                                    >  1:399776/1‑251 (MQ=255)                                                                                           tttCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTg                                                                                                                                                                                 >  1:653808/1‑217 (MQ=255)                                                                                              ccACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGAt                                                                                                                                                                               >  1:51803/1‑216 (MQ=255)                                                                                                         cAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                                                                                  <  1:840182/250‑1 (MQ=255)                                                                                                              gATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGGGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGcc                                                                                                                                                                                      >  1:66129/1‑193 (MQ=255)                                                                                                               aTTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCa                                                                                                                                                                                                                             <  1:691972/153‑1 (MQ=255)                                                                                                                    aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:751193/251‑1 (MQ=255)                                                                                                                    aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:21401/251‑1 (MQ=255)                                                                                                                    aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:556168/251‑1 (MQ=255)                                                                                                                         ggCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:264212/246‑1 (MQ=255)                                                                                                                                cAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGc                                                                                                          <  1:321486/251‑1 (MQ=255)                                                                                                                                    tCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTAt                                                                                                       >  1:520947/1‑250 (MQ=255)                                                                                                                                          aTCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                <  1:336499/251‑1 (MQ=255)                                                                                                                                             tGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGataat                                                                                             <  1:605766/251‑1 (MQ=255)                                                                                                                                              gTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATtat                                                                                                                                                                            >  1:96932/1‑171 (MQ=255)                                                                                                                                              gTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      >  1:876365/1‑225 (MQ=255)                                                                                                                                               tttATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatat                                                                                           <  1:387344/251‑1 (MQ=255)                                                                                                                                                      gCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGc                                                                                        >  1:501454/1‑247 (MQ=255)                                                                                                                                                         tGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATTTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATta                                                                                                                                                                             <  1:193333/159‑1 (MQ=255)                                                                                                                                                             tATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTc                                                                             <  1:290769/251‑1 (MQ=255)                                                                                                                                                                      caaTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCGGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                                                                                  <  1:561691/189‑1 (MQ=255)                                                                                                                                                                      caaTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCt                                                                                                                                       <  1:809451/184‑1 (MQ=255)                                                                                                                                                                        atatTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatat                                                                                           <  1:411796/226‑1 (MQ=255)                                                                                                                                                                        atatTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGcacc                                                                   >  1:401896/1‑247 (MQ=255)                                                                                                                                                                             gACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                >  1:275605/1‑216 (MQ=255)                                                                                                                                                                                   cTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGcc                                                                                                                                        >  1:245499/1‑170 (MQ=255)                                                                                                                                                                                    tACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCaggag                                                      <  1:797333/251‑1 (MQ=255)                                                                                                                                                                                    tACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCaggag                                                      <  1:74341/251‑1 (MQ=255)                                                                                                                                                                                         gAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGAcg                                                  >  1:581203/1‑250 (MQ=255)                                                                                                                                                                                           aaccaaccCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                                                                                  >  1:868593/1‑168 (MQ=255)                                                                                                                                                                                                          aagatatCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTc                                                                                                                                                     >  1:239604/4‑134 (MQ=255)                                                                                                                                                                                                           aaaTATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTaaagt                               >  1:324203/1‑248 (MQ=255)                                                                                                                                                                                                           aaaTATCAGTTAATACTACTAATGGATGAGTTTACTACCCTCGATATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCAATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:278555/164‑1 (MQ=255)                                                                                                                                                                                                             atatCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                >  1:735325/1‑184 (MQ=255)                                                                                                                                                                                                                 cAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAgg                         >  1:281098/1‑251 (MQ=255)                                                                                                                                                                                                                   gTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTa                       >  1:509854/1‑251 (MQ=255)                                                                                                                                                                                                                    ttattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGt                        <  1:601565/249‑1 (MQ=255)                                                                                                                                                                                                                    ttattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTa                       <  1:264366/250‑1 (MQ=255)                                                                                                                                                                                                                    ttattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACGTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATAGGCCCCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGt                        <  1:491335/249‑1 (MQ=255)                                                                                                                                                                                                                     tattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                <  1:75807/176‑1 (MQ=255)                                                                                                                                                                                                                      attaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCt                                    <  1:522706/235‑1 (MQ=255)                                                                                                                                                                                                                        tactacTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTc                                     <  1:502246/232‑1 (MQ=255)                                                                                                                                                                                                                          ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAggg                                                                                                                                                            >  1:860830/1‑111 (MQ=255)                                                                                                                                                                                                                          ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:399882/149‑1 (MQ=255)                                                                                                                                                                                                                          ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCAc                <  1:597058/251‑1 (MQ=255)                                                                                                                                                                                                                          ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCAc                <  1:153221/251‑1 (MQ=255)                                                                                                                                                                                                                               aaTGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAatat                                       >  1:118976/2‑223 (MQ=255)                                                                                                                                                                                                                                    aTGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGtaaataaa      <  1:871369/251‑1 (MQ=255)                                                                                                                                                                                                                                     tgagtttACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGATGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTaaataaat     <  1:663503/247‑1 (MQ=255)                                                                                                                                                                                                                                        gtttACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTaa  >  1:834656/2‑251 (MQ=255)                                                                                                                                                                                                                                        gtttACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTaa  >  1:728261/2‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            AACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATTTTGATTTACGCGCCTTGCGCCGGGAAAAAATCACGATTTATTTCCACATTTCAGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCGGCATTAAAATATCAGTTATTACTACTCATGGATGAATTTACTGCGGTCGGGATGTTGAGCATTATCAATAAATCAGTAGGATTTATTGCCGGTTATGGTTTACGCCTATTGATTATCTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCCCCCGACAATCAGGAGGACGCGGAAGAATATTCTAAGGTGCTAGGTAATCGCACTGTAAATAAATTAA  >  1/777289‑777770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑