Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	777,157	G→A	intergenic (+42284/+44676)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	777,157	0	G	A	100.0%	211.6 / NA	64	intergenic (+42284/+44676)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/27);  total (37/27)

TTAAAAAATCAGGCAATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAAAATCTTTTTGTGGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACTGATATGACACTGACTTACGCCAATCTACTACGTTTACACGCAGGTTTTGACGAAACGCAAGAAGACGGGTCAATAGAACATATCGATTTTGCGCAACACGCGCGGTTATGCGTTCAATCCGGCATTGCTCACCAAACTACCGCCGATAAACTCAATATTTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAGTAGTATCGATTCAACTTTTACCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATTTTGATTTACGCGCCTTGCGCCGGGAAAAAATCACGATTTATTTCCACATTTCA  >  1/776933‑777391                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           ttAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATc                                                                                                                                                                                                                  <  1:284332/251‑1 (MQ=255)     aaaaTCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGc                                                                                                                                                                                                              <  1:16269/251‑1 (MQ=255)      aaaTCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCa                                                                                                                                                                                                             >  1:842762/1‑251 (MQ=255)       aaTCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCAt                                                                                                                                                                                                            <  1:9621/251‑1 (MQ=255)            ggctaTGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCt                                                                                                                                                                                                        >  1:607405/5‑250 (MQ=255)                   ggCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGt                                                                                                                                                                                                                        <  1:414075/227‑1 (MQ=255)                   ggCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCaga                                                                                                                                                                                                 >  1:816676/1‑248 (MQ=255)                   ggCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCag                                                                                                                                                                                                  >  1:72784/1‑248 (MQ=255)                    gCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACAtgcgcgc                                                                                                                                                                                                                                >  1:178253/1‑217 (MQ=255)                    gCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCaga                                                                                                                                                                                                 >  1:512315/1‑247 (MQ=255)                                         aCCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAatat                                                                                                                                                                           <  1:120957/250‑1 (MQ=255)                                              gATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATatttattt                                                                                                                                                                     >  1:491882/1‑251 (MQ=255)                                                    gACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAatat                                                                                                                                                                           <  1:630242/239‑1 (MQ=255)                                                          tGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCAt                                                                                                                                                                                                            >  1:561728/1‑200 (MQ=255)                                                             cagcaCCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAAt                                                                                                                                                       <  1:371999/250‑1 (MQ=255)                                                               gcaCCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTActgc                                                                                                                                                                                          >  1:185231/1‑210 (MQ=255)                                                                             aTCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAagc                                                                                                                                      <  1:276096/251‑2 (MQ=255)                                                                                         cAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGAtt                                                                                                                            <  1:342797/249‑1 (MQ=255)                                                                                               cTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATcc                                                                                                                                                                                                                 <  1:714261/158‑1 (MQ=255)                                                                                               cTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATcc                                                                                                                                                     <  1:641055/218‑1 (MQ=255)                                                                                                 tttttACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTat                                                                                                                  >  1:661828/1‑250 (MQ=255)                                                                                                  ttttACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTatc                                                                                                                 <  1:389396/251‑3 (MQ=255)                                                                                                         gtgGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAACCAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGt                                                                                                          >  1:518286/1‑251 (MQ=255)                                                                                                         gtgGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTACCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGt                                                                                                          >  1:438555/1‑251 (MQ=255)                                                                                                              atatGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAAc                                                                                                      <  1:95731/250‑1 (MQ=255)                                                                                                                      cAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAAcc                                                                                                                       >  1:211575/1‑224 (MQ=255)                                                                                                                                  accgATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAAttgt                                                                                 >  1:365137/4‑251 (MQ=255)                                                                                                                                      atatGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTc                                                                                                                                                                                <  1:342916/152‑1 (MQ=255)                                                                                                                                      atatGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCCGTCCGTTAACCCTTTTTCAAAATGAAATTGTTGaa                                                                             >  1:643498/1‑251 (MQ=255)                                                                                                                                      atatGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGaa                                                                             >  1:774546/1‑251 (MQ=255)                                                                                                                                      atatGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCATGTGAAt                                                                                                                                                       >  1:435339/1‑177 (MQ=255)                                                                                                                                           atacTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAAt                                                                                                                                                                  <  1:250877/159‑1 (MQ=255)                                                                                                                                           atacTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGAtt                                                                                                                            >  1:277133/3‑199 (MQ=255)                                                                                                                                           atacTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATg                                                                         >  1:894784/3‑250 (MQ=255)                                                                                                                                           atacTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATg                                                                         >  1:316817/3‑250 (MQ=255)                                                                                                                                           atacTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATg                                                                         >  1:223346/3‑250 (MQ=255)                                                                                                                                               tgacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACt                                                                    <  1:737168/243‑1 (MQ=255)                                                                                                                                                gacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAAtatttatt                                                                                                                                                                      <  1:292839/145‑1 (MQ=255)                                                                                                                                                gacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATgg                                                                                                                                                                >  1:278334/8‑158 (MQ=255)                                                                                                                                                gacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTatc                                                                                                                 >  1:670919/8‑203 (MQ=255)                                                                                                                                                gacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTc                                                                                              >  1:411756/8‑224 (MQ=255)                                                                                                                                                gacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACtt                                                                   >  1:495585/8‑251 (MQ=255)                                                                                                                                                     atgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGcc                                                              <  1:421074/249‑1 (MQ=255)                                                                                                                                                     atgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGcc                                                              <  1:209195/249‑1 (MQ=255)                                                                                                                                                         cAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGtg                                                          <  1:5759/251‑1 (MQ=255)                                                                                                                                                            tCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGtgatt                                                       >  1:517494/1‑251 (MQ=255)                                                                                                                                                             ctattacGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTatc                                                                                                                 <  1:172002/188‑3 (MQ=255)                                                                                                                                                             ctattacGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGtgattt                                                      >  1:581430/5‑251 (MQ=255)                                                                                                                                                             ctattacGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGtgattt                                                      >  1:863550/5‑251 (MQ=255)                                                                                                                                                             ctattacGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTACCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGtgattt                                                      >  1:876410/5‑251 (MQ=255)                                                                                                                                                                     cttACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCa                                                                                                    <  1:635351/196‑1 (MQ=255)                                                                                                                                                                     cttACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTa                                               >  1:102064/2‑250 (MQ=255)                                                                                                                                                                       tacatgccggTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTAcgc                                            <  1:108485/243‑1 (MQ=255)                                                                                                                                                                                gTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACgcgctttacg                                    >  1:382383/1‑244 (MQ=255)                                                                                                                                                                                      atgAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACgcgctttacgcaaggaa                             >  1:282996/3‑238 (MQ=255)                                                                                                                                                                                      atgAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACgcgctttacgcaaggaa                             >  1:428168/3‑238 (MQ=255)                                                                                                                                                                                              cAAGAAGACGGTTCGATAGAACATATCGTTTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAACAGTATCGATTCAACCTTTATCAGTCCGCTAACCATTTTTCAAAATGAAATTGTTGAATTTGCGACTTCTGCCCGTGATTTTGTTTTACGCGCTTTACGCAAGGAAAAAATcc                      >  1:608590/1‑249 (MQ=255)                                                                                                                                                                                              cAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTatc                                                                                                                 <  1:186754/159‑3 (MQ=255)                                                                                                                                                                                              cAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCa                      >  1:374650/1‑250 (MQ=255)                                                                                                                                                                                                        gttcgaTAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAAcc                                                                                                                       >  1:148184/6‑142 (MQ=255)                                                                                                                                                                                                           cgaTAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAAcccttatcagtc                                                                                                             <  1:94922/148‑12 (MQ=255)                                                                                                                                                                                                             aTAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACAt      <  1:792956/251‑1 (MQ=255)                                                                                                                                                                                                                 aaCATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTcg  >  1:593985/1‑250 (MQ=255)                                                                                                                                                                                                                          aTTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGAc                                                                     <  1:478147/175‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           TTAAAAAATCAGGCAATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAAAATCTTTTTGTGGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACTGATATGACACTGACTTACGCCAATCTACTACGTTTACACGCAGGTTTTGACGAAACGCAAGAAGACGGGTCAATAGAACATATCGATTTTGCGCAACACGCGCGGTTATGCGTTCAATCCGGCATTGCTCACCAAACTACCGCCGATAAACTCAATATTTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAGTAGTATCGATTCAACTTTTACCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATTTTGATTTACGCGCCTTGCGCCGGGAAAAAATCACGATTTATTTCCACATTTCA  >  1/776933‑777391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑