Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	776,830	G→A	intergenic (+41957/+45003)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	776,830	0	G	A	100.0%	124.1 / NA	37	intergenic (+41957/+45003)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/20);  total (17/20)

ATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTTGGTAATAAACTTCTTCGCTTTATCGGTGCACAATTCGTTTCGTTGGCCGCACCGACCCGAGGTGGGAAAGGAATAGGGATTGTCATACCGAATTTATTAGAGTGGCAACAATCAGTTGTTGTGAAAGATGTTAAAAACGAATGTTATCGCATTACCAGTAAATACCGTCAAAAAATATTGGGGCAAAAAGTCTATCGCTTTGCCCCATTTGACCGTAACACGCACCGATTTAATCCATTGGATTGTATTGATATGCGCGACCGAGCACGCGCCGAACTCGATTTAAAAAATCAGGCAATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAAAATCTTTTTGTGGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACTGATATGACAC  >  1/776590‑777074                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     aTCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCaaaagg                                                                                                                                                                                                                                            >  1:163853/1‑249 (MQ=255)      aaGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCaaaaggtatat                                                                                                                                                                                                                                       <  1:877799/251‑8 (MQ=255)        gTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCg                                                                                                                                                                                                                                     >  1:410405/1‑251 (MQ=255)         tACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCg                                                                                                                                                                                                                                     <  1:71111/250‑1 (MQ=255)                                                                                                                                                   ttccaaatttgctggcatgggaacaaTCTGTGGTTGTGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGAc                                                                                          <  1:658709/230‑1 (MQ=255)                                                                                                                                                                  catgggaacaaTCTGTGGTTGTGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATc                                                                                                                    >  1:761916/7‑210 (MQ=255)                                                                                                                                                                       gaacaaTCTGTGGTTGTGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGcac                                                                       <  1:601535/249‑1 (MQ=255)                                                                                                                                                                            atctgtggttgtGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTatat                                                                                                                     >  1:709998/8‑199 (MQ=255)                                                                                                                                                                            atctgtggttgtGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAAt                                                                  >  1:407013/8‑250 (MQ=255)                                                                                                                                                                                gtggttgtGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCtttt                                                             >  1:222724/4‑251 (MQ=255)                                                                                                                                                                                   gttgtGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGAt                                                                                                <  1:489360/213‑1 (MQ=255)                                                                                                                                                                                   gttgtGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTtgt                                                          >  1:463565/1‑251 (MQ=255)                                                                                                                                                                                     tgtgaaggacattAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTtgtag                                                        <  1:636583/240‑3 (MQ=255)                                                                                                                                                                                      gtgaaggacattAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATc                                                                                                                    >  1:177614/11‑190 (MQ=255)                                                                                                                                                                                              cattAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGc                                                                                                                           >  1:630866/3‑175 (MQ=255)                                                                                                                                                                                              cattAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATc                                                                                                                    >  1:353291/3‑182 (MQ=255)                                                                                                                                                                                              cattAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACtt                                               >  1:795126/3‑251 (MQ=255)                                                                                                                                                                                                    aaaCGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTAc                                         <  1:806529/251‑1 (MQ=255)                                                                                                                                                                                                    aaaCGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTAc                                         <  1:779473/251‑1 (MQ=255)                                                                                                                                                                                                    aaaCGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTAc                                         <  1:241414/251‑1 (MQ=255)                                                                                                                                                                                                          atgctATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTatat                                                                                                                     <  1:715876/165‑1 (MQ=255)                                                                                                                                                                                                             ctATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGata                                >  1:876069/2‑251 (MQ=255)                                                                                                                                                                                                                 cgtatcaCCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTatat                                                                                                                     <  1:40005/156‑1 (MQ=255)                                                                                                                                                                                                                 cgtatcaCCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTAc                                         <  1:864890/232‑1 (MQ=255)                                                                                                                                                                                                                 cgtatcaCCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATgcg                            >  1:170613/7‑251 (MQ=255)                                                                                                                                                                                                                   tatcaCCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATgcgc                           <  1:796497/246‑1 (MQ=255)                                                                                                                                                                                                                           gTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTAAATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAg                   >  1:119774/1‑250 (MQ=255)                                                                                                                                                                                                                             aaaTATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGc                  >  1:356409/1‑249 (MQ=255)                                                                                                                                                                                                                              aatatcgacAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCt                                                                                                                          <  1:63099/136‑1 (MQ=255)                                                                                                                                                                                                                                  tcgacAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTAccg           <  1:84567/247‑3 (MQ=255)                                                                                                                                                                                                                                          aaaTATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACTCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTAc                                         <  1:157528/213‑1 (MQ=255)                                                                                                                                                                                                                                          aaaTATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGata   >  1:286276/1‑249 (MQ=255)                                                                                                                                                                                                                                           aaTATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGatac  <  1:300752/251‑4 (MQ=255)                                                                                                                                                                                                                                           aaTATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGatac  <  1:897884/251‑4 (MQ=255)                                                                                                                                                                                                                                            atatTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGatac  <  1:411677/250‑4 (MQ=255)                                                                                                                                                                                                                                            atatTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGata   >  1:858618/1‑247 (MQ=255)                                                                                                                                                                                                                                             tatTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGatac  <  1:772374/249‑4 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     ATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTTGGTAATAAACTTCTTCGCTTTATCGGTGCACAATTCGTTTCGTTGGCCGCACCGACCCGAGGTGGGAAAGGAATAGGGATTGTCATACCGAATTTATTAGAGTGGCAACAATCAGTTGTTGTGAAAGATGTTAAAAACGAATGTTATCGCATTACCAGTAAATACCGTCAAAAAATATTGGGGCAAAAAGTCTATCGCTTTGCCCCATTTGACCGTAACACGCACCGATTTAATCCATTGGATTGTATTGATATGCGCGACCGAGCACGCGCCGAACTCGATTTAAAAAATCAGGCAATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAAAATCTTTTTGTGGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACTGATATGACAC  >  1/776590‑777074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑