Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	776,309	G→A	intergenic (+41436/+45524)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	776,309	0	G	A	100.0%	286.2 / NA	83	intergenic (+41436/+45524)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/41);  total (42/41)

CCTCTTGTCATGCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCACCGTTTATTACAACAAACGATAGATATTATCGTGCATGTTACCAACAGTCACGAATACGGCAGACATATGACGGAAATCTATTTTGATCCGCAAGCCAAACTGAACTCTCTCAAGGTGGATTAACTATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGATTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCA  >  1/776092‑776547                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               ccTCTTGTCATGCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCattat                                                                                                                                                                                                               >  1:163603/1‑251 (MQ=255)    ctTGTCATGCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTcc                                                                                                                                                                                                            <  1:723031/251‑1 (MQ=255)        tCATGCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGtt                                                                                                                                                                                                        <  1:880489/251‑1 (MQ=255)          aTGCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTg                                                                                                                                                                                                      <  1:226606/251‑1 (MQ=255)                           gAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACg                                                                                                                                                                                     >  1:657977/1‑251 (MQ=255)                               ccTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCTGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGCAGGTTAATGATGACAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTAtt                                                                                                                                                                                 >  1:210393/1‑251 (MQ=255)                                    gAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCt                                                                                                                                                                             <  1:175190/250‑1 (MQ=255)                                         gCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTgg                                                                                                                                                                       >  1:754125/1‑251 (MQ=255)                                             aTTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCat                                                                                                                                                                                                                  <  1:322280/204‑1 (MQ=255)                                                      aCGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGAc                                                                                                                                                                                      <  1:871161/223‑1 (MQ=255)                                                       cGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTAc                                                                                                                                                         <  1:156920/251‑1 (MQ=255)                                                       cGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTAc                                                                                                                                                         <  1:541992/251‑1 (MQ=255)                                                                aCGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTAc                                                                                                                                                               >  1:233367/1‑236 (MQ=255)                                                                  gACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATaaa                                                                                                                                              >  1:206298/1‑251 (MQ=255)                                                                                 cTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGaa                                                                                                                               >  1:510588/1‑251 (MQ=255)                                                                                   ttCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACtt                                                                                                                                                             >  1:578642/1‑219 (MQ=255)                                                                                   ttCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAg                                                                                                                              >  1:828948/1‑250 (MQ=255)                                                                                     ccTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCAt                                                                                                                           >  1:626452/1‑251 (MQ=255)                                                                                     ccTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCAt                                                                                                                           >  1:748827/1‑251 (MQ=255)                                                                                        atgatgTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTa                                                                                                                        <  1:207922/251‑1 (MQ=255)                                                                                          gatgTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAg                                                                                                                      >  1:748015/1‑251 (MQ=255)                                                                                           atgTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAg                                                                                                                      <  1:272375/250‑1 (MQ=255)                                                                                             gTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAgcg                                                                                                                    >  1:43524/1‑250 (MQ=255)                                                                                              gttcatgactgttggtaacatgaaCGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAgcg                                                                                                                    >  1:770177/23‑249 (MQ=255)                                                                                                     ccGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTAccc                                                                                                           <  1:245743/251‑1 (MQ=255)                                                                                                     ccGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTAccc                                                                                                           <  1:825594/251‑1 (MQ=255)                                                                                                       gTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAg                                                                                                                                                                          >  1:444681/1‑186 (MQ=255)                                                                                                       gTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCtactac                                                                                                             <  1:806219/247‑1 (MQ=255)                                                                                                       gTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTa                                                                                                         >  1:97979/1‑251 (MQ=255)                                                                                                          tattaCAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTActc                                                                                                      <  1:220782/251‑1 (MQ=255)                                                                                                               caacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACg                                                                                                 >  1:9114/1‑251 (MQ=255)                                                                                                               caacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACg                                                                                                 >  1:428028/1‑251 (MQ=255)                                                                                                                aacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                <  1:78874/251‑1 (MQ=255)                                                                                                                aacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                <  1:316111/251‑1 (MQ=255)                                                                                                                aacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                <  1:160197/251‑1 (MQ=255)                                                                                                                      cGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTaa                                                                                                   <  1:119886/242‑1 (MQ=255)                                                                                                                      cGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATg                                                                                          >  1:597030/1‑251 (MQ=255)                                                                                                                      cGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATg                                                                                          >  1:532029/1‑251 (MQ=255)                                                                                                                           gataTTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCAc                                                                                                                                                                                                  >  1:232454/1‑142 (MQ=255)                                                                                                                            atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTcc                                                                                                                                                                                                            >  1:849068/1‑131 (MQ=255)                                                                                                                            atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCAc                                                                                                                                                                                                  <  1:62059/141‑1 (MQ=255)                                                                                                                            atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACtt                                                                                                                                                                                                <  1:429416/143‑1 (MQ=255)                                                                                                                            atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTAc                                                                                                        >  1:286458/1‑231 (MQ=255)                                                                                                                            atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAttt                                                                                    >  1:751286/1‑251 (MQ=255)                                                                                                                            atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAttt                                                                                    <  1:253896/251‑1 (MQ=255)                                                                                                                               ttattgttcATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCat                                                                                                                                                                                                                  >  1:66324/9‑122 (MQ=255)                                                                                                                               ttattgttcATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                <  1:712633/228‑1 (MQ=255)                                                                                                                               ttattgttcATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTggg                                                                                 >  1:177848/9‑251 (MQ=255)                                                                                                                                 attgttcATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                >  1:207709/7‑234 (MQ=255)                                                                                                                                 attgttcATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                <  1:415719/228‑1 (MQ=255)                                                                                                                                      tcATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTa                                                                          <  1:9967/250‑1 (MQ=255)                                                                                                                                         tGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATc                                                                        >  1:161612/1‑250 (MQ=255)                                                                                                                                          gTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCg                                                                      >  1:30557/1‑251 (MQ=255)                                                                                                                                            tACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTAc                                                                                                                                                         <  1:270525/166‑1 (MQ=255)                                                                                                                                            tACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGaa                                                                    <  1:581010/251‑1 (MQ=255)                                                                                                                                              ccAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGaaaa                                                                  >  1:320157/1‑251 (MQ=255)                                                                                                                                                 aCAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAatat                                                               >  1:361354/1‑251 (MQ=255)                                                                                                                                                    gTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAat                                                                 >  1:782245/1‑246 (MQ=255)                                                                                                                                                    gTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATgg                                                             >  1:642513/1‑250 (MQ=255)                                                                                                                                                           acaCGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAgc                                                                                                                     >  1:10082/3‑187 (MQ=255)                                                                                                                                                              cGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTAc                                                                                                                                                               <  1:841435/142‑1 (MQ=255)                                                                                                                                                              cGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGtctctc                                                    >  1:318898/1‑249 (MQ=255)                                                                                                                                                              cGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGtctctc                                                    >  1:344569/1‑249 (MQ=255)                                                                                                                                                              cGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCt                                                  <  1:543871/251‑1 (MQ=255)                                                                                                                                                                    acacatGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTATCCATATTAg                                            <  1:869315/247‑1 (MQ=255)                                                                                                                                                                     cacatGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGTATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAGGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGt                                           <  1:443342/248‑1 (MQ=255)                                                                                                                                                                     cacatGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                <  1:82953/195‑1 (MQ=255)                                                                                                                                                                     cacatGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                >  1:900208/4‑198 (MQ=255)                                                                                                                                                                       catGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTGATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGttt                                         >  1:463098/2‑251 (MQ=255)                                                                                                                                                                       catGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGttt                                         >  1:786281/2‑251 (MQ=255)                                                                                                                                                                            cGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGTTTTGTAg                                    <  1:392351/251‑1 (MQ=255)                                                                                                                                                                            cGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGTTTTGTAg                                    <  1:486176/251‑1 (MQ=255)                                                                                                                                                                                aaTCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTa                                                                                                                                                                            <  1:524069/111‑1 (MQ=255)                                                                                                                                                                                aaTCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTcc                                                   <  1:276380/232‑1 (MQ=255)                                                                                                                                                                                   cTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGt                                           >  1:314391/1‑237 (MQ=255)                                                                                                                                                                                        ttGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGTTTTGTAGTGAGCTTACTaa                        <  1:390015/251‑1 (MQ=255)                                                                                                                                                                                        ttGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGTTTTGTAGTGAGCTTACTaa                        <  1:573678/251‑1 (MQ=255)                                                                                                                                                                                           aTCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGTTTTGTAGTGAGCTTACTAATCa                     <  1:888903/251‑1 (MQ=255)                                                                                                                                                                                                  agctaaaCTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTc                                                                                                                                                                                                             >  1:547398/5‑60 (MQ=255)                                                                                                                                                                                                  agctaaaCTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGTTTTGTAGTGAGCTTACTAATCATGCTGaa              >  1:745742/5‑251 (MQ=255)                                                                                                                                                                                                    ctaaaCTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAAc            <  1:366699/249‑1 (MQ=255)                                                                                                                                                                                                      aaaCTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTAc                                                                                                                                                               <  1:565317/102‑1 (MQ=255)                                                                                                                                                                                                              ttcgctcAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCa  <  1:476304/247‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               CCTCTTGTCATGCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCACCGTTTATTACAACAAACGATAGATATTATCGTGCATGTTACCAACAGTCACGAATACGGCAGACATATGACGGAAATCTATTTTGATCCGCAAGCCAAACTGAACTCTCTCAAGGTGGATTAACTATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGATTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTTATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCA  >  1/776092‑776547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑