Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	768,021	T→C	intergenic (+33148/+53812)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	768,021	0	T	C	100.0%	213.8 / NA	63	intergenic (+33148/+53812)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/39);  total (24/39)

CTTTTGGGATGTCTTCGGCGAAACCGGCATTCCGCTGCGCACCACCATTTCCGAAATGGGGCCGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCTAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGA  >  1/767775‑768254                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          cTTTTGGGATGTCTTCGGCGAAACCGGCATTCCGCTGCGCACCACCATTTCCGAAATGGGGCCGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCg                                                                                                                                                                                                                                       >  1:266085/1‑251 (MQ=255)     tGGGATGTCTTCGGCGAAACCGGCATTCCGCTGCGCACCACCATTTCCGAAATGGGGCCGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTggg                                                                                                                                                                                                                                   <  1:818717/251‑1 (MQ=255)                    gAAACCGGCATTCCGCTGCGCACCACCATTTCCGAAATGGGGCCGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACggg                                                                                                                                                                                                                     >  1:764251/1‑250 (MQ=255)                     aaaCCGGCATTCCGCTGCGCACCACCATTTCCGAAATGGGGCCGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCa                                                                                                                                                                                                                   <  1:474255/251‑1 (MQ=255)                           gCATTCCGCTGCGCACCACCATTTCCGAAATGGGGCCGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCattatt                                                                                                                                                                                                              <  1:41937/250‑1 (MQ=255)                               tCCGCTGCGCACCACCATTTCCGAAATGGGGCCGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGc                                                                                                                                                                                                         <  1:575472/251‑1 (MQ=255)                                     gcgcACCACCATTTCCGAAATGGGGCCGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCg                                                                                                                                                                                                                                       >  1:379402/1‑215 (MQ=255)                                     gcgcACCACCATTTCCGAAATGGGGCCGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAg                                                                                                                                                                                                                                 <  1:536768/221‑1 (MQ=255)                                                               cGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAgg                                                                                                                                                                                            >  1:611548/1‑232 (MQ=255)                                                                gttgttGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGaa                                                                                                                                                                                              >  1:525576/1‑229 (MQ=255)                                                                 ttgttgCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGa                                                                                                                                                                       >  1:600822/1‑251 (MQ=255)                                                                                   ttGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGa                                                                                                                                                     <  1:339281/251‑1 (MQ=255)                                                                                       aTTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGAc                                                                                                                                                 <  1:819376/251‑1 (MQ=255)                                                                                       aTTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGAc                                                                                                                                                 <  1:622825/251‑1 (MQ=255)                                                                                         ttGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGa                                                                                                                                                  >  1:831955/1‑248 (MQ=255)                                                                                                           ggCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACgg                                                                                                                             >  1:201059/1‑251 (MQ=255)                                                                                                           ggCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACgg                                                                                                                             >  1:676521/1‑251 (MQ=255)                                                                                                            gCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACg                                                                                                                              <  1:301014/249‑1 (MQ=255)                                                                                                                   cAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCgtggtg                                                                                                                     <  1:851620/251‑1 (MQ=255)                                                                                                                    aaTCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCattatt                                                                                                                                                                                                              <  1:769456/161‑1 (MQ=255)                                                                                                                     aTCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGgtgt                                                                                                                    >  1:634717/1‑250 (MQ=255)                                                                                                                       cTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGc                                                                                                                                          <  1:44984/226‑1 (MQ=255)                                                                                                                          gtgtTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGAc                                                                                                                                                 <  1:329405/216‑1 (MQ=255)                                                                                                                           tgtTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAAt                                                                                                             <  1:453958/251‑1 (MQ=255)                                                                                                                                 gcgTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCaaa                                                                                                                                       <  1:517186/219‑1 (MQ=255)                                                                                                                                      gCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCGAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCattatt                                                                                                                                                                                                              <  1:57130/143‑1 (MQ=255)                                                                                                                                                  ggCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAAt                                                                                                             >  1:633445/1‑228 (MQ=255)                                                                                                                                                  ggCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGAt                                                                                      >  1:644116/1‑251 (MQ=255)                                                                                                                                                    cTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATc                                                                                     <  1:426015/250‑1 (MQ=255)                                                                                                                                                      tATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAAt                                                                                  <  1:481522/251‑1 (MQ=255)                                                                                                                                                                  aCTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTAAAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATg                                                                      >  1:439852/1‑251 (MQ=255)                                                                                                                                                                     tAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTc                                                                                                                                                                            <  1:748558/146‑1 (MQ=255)                                                                                                                                                                     tAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGa                                                                                                                                                     >  1:890936/1‑169 (MQ=255)                                                                                                                                                                     tAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTAc                                                                   <  1:104539/251‑1 (MQ=255)                                                                                                                                                                     tAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTAc                                                                   <  1:278712/251‑1 (MQ=255)                                                                                                                                                                       aaaGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAg                                                                 <  1:242024/251‑1 (MQ=255)                                                                                                                                                                       aaaGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAg                                                                 <  1:698798/251‑1 (MQ=255)                                                                                                                                                                           aTCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGcc                                                             <  1:251472/251‑1 (MQ=255)                                                                                                                                                                                    ccATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCtt                                                    <  1:162629/251‑1 (MQ=255)                                                                                                                                                                                      aTGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAg                                                                 >  1:372938/1‑236 (MQ=255)                                                                                                                                                                                       tGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTgg                                                 <  1:450890/251‑1 (MQ=255)                                                                                                                                                                                        gTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAgcgc                                                              >  1:279619/1‑237 (MQ=255)                                                                                                                                                                                        gTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGt                                                >  1:533820/1‑251 (MQ=255)                                                                                                                                                                                         ttGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGtt                                               <  1:406479/251‑1 (MQ=255)                                                                                                                                                                                           gAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTAc                                                                   <  1:83342/229‑1 (MQ=255)                                                                                                                                                                                           gAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAg                                                                 <  1:196878/231‑1 (MQ=255)                                                                                                                                                                                                atGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCg                                        >  1:659705/1‑251 (MQ=255)                                                                                                                                                                                                          aaaTGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTg                              <  1:593655/251‑1 (MQ=255)                                                                                                                                                                                                             tGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTACTGGCGGAATTATTTGAg                            >  1:113645/1‑250 (MQ=255)                                                                                                                                                                                                             tGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGc                           <  1:58579/251‑1 (MQ=255)                                                                                                                                                                                                                       ttCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGa                 <  1:723639/251‑1 (MQ=255)                                                                                                                                                                                                                       ttCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGa                 <  1:523140/251‑1 (MQ=255)                                                                                                                                                                                                                       ttCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGa                 <  1:192657/251‑1 (MQ=255)                                                                                                                                                                                                                         ccAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAg               <  1:262915/251‑1 (MQ=255)                                                                                                                                                                                                                             gTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAATGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGtt                                               <  1:451884/215‑1 (MQ=255)                                                                                                                                                                                                                                gAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCgtggtg                                                                                                                     >  1:452094/1‑142 (MQ=255)                                                                                                                                                                                                                                gAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGa        >  1:741800/1‑251 (MQ=255)                                                                                                                                                                                                                                    aCGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGAccc     >  1:800152/1‑250 (MQ=255)                                                                                                                                                                                                                                     cGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAAt                                                                                                             <  1:96056/145‑1 (MQ=255)                                                                                                                                                                                                                                       gTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTAc                                                                   <  1:882539/185‑1 (MQ=255)                                                                                                                                                                                                                                       gTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGa  >  1:343641/1‑250 (MQ=255)                                                                                                                                                                                                                                       gTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGa  >  1:147833/1‑250 (MQ=255)                                                                                                                                                                                                                                              aTCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGaaa                                                                                             <  1:394359/152‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          CTTTTGGGATGTCTTCGGCGAAACCGGCATTCCGCTGCGCACCACCATTTCCGAAATGGGGCCGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCTAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGA  >  1/767775‑768254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑