Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	688,989	A→G	intergenic (+198074/‑7719)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	688,989	0	A	G	100.0%	152.5 / NA	45	intergenic (+198074/‑7719)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/27);  total (18/27)

ACGTGCTGGTGGCTACACCCGCATCCTTAAATGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAACTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTGTAAGTATCACATCGTCTTCACCCCGAAATATAGAAGAAAAGCCATTTACGGAAG  >  1/688748‑689177                                                                                                                                                                                                                                                  |                                                                                                                                                                                             aCGTGCTGGTGGCTACACCCGCATCCTTAAATGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCgg                                                                                                                                                                                     >  1:818114/1‑251 (MQ=255)   gTGCTGGTGGCTACACCCGCATCCTTAAATGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGt                                                                                                                                                                                    >  1:69450/1‑250 (MQ=255)                      cATCCTTAAATGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCa                                                                                                                                                                  >  1:794674/1‑249 (MQ=255)                           ttAAATGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACtatata                                                                                                                                                           <  1:10033/251‑1 (MQ=255)                            tAAATGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATaa                                                                                                                                                          >  1:231748/1‑251 (MQ=255)                               aTGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGaa                                                                                                                                                       <  1:775814/251‑1 (MQ=255)                               aTGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGaa                                                                                                                                                       <  1:295852/251‑1 (MQ=255)                               aTGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGaa                                                                                                                                                       <  1:738956/251‑1 (MQ=255)                               aTGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGaa                                                                                                                                                       <  1:609374/251‑1 (MQ=255)                                   ggTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAg                                                                                                                                                         <  1:254774/245‑1 (MQ=255)                                     tttCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGc                                                                                                                                                 <  1:292734/251‑1 (MQ=255)                                                      aTGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGc                                                                                                                                                 <  1:54136/234‑1 (MQ=255)                                                        gCTCCAATAGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGa                                                                                                                              >  1:838272/1‑251 (MQ=255)                                                         cTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACtat                                                                                                                                                              >  1:642860/1‑218 (MQ=255)                                                                ggCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAg                                                                                                                                                         <  1:149676/216‑1 (MQ=255)                                                                   ttACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGAt                                                                                                                   <  1:501669/251‑1 (MQ=255)                                                                     aCATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAGAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATaa                                                                                                                 >  1:435046/1‑251 (MQ=255)                                                                          gAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGc                                                                                                                                                                                       <  1:710447/176‑1 (MQ=255)                                                                          gAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGaa                                                                                                                                                       <  1:312703/208‑1 (MQ=255)                                                                          gAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGAt                                                                                                                   >  1:193823/1‑244 (MQ=255)                                                                          gAGTTAGTAGATCGTCCAGAAGTTGCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATcc                                                                                                                                                                     <  1:443071/194‑1 (MQ=255)                                                                           aGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAg                                                                                                                                                         <  1:214037/205‑1 (MQ=255)                                                                           aGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGaa                                                                                                                                                       >  1:414642/1‑207 (MQ=255)                                                                            gTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATaggag                                                                                                                      >  1:745500/1‑239 (MQ=255)                                                                             ttAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGTGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATgg                                                                                                         <  1:649902/251‑1 (MQ=255)                                                                                        tCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGc                                                                                               >  1:416228/1‑250 (MQ=255)                                                                                                      gTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAg                                                                                >  1:748125/1‑251 (MQ=255)                                                                                                             agCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATca                                                                         >  1:633724/1‑251 (MQ=255)                                                                                                               cTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATcac                                                                        <  1:6201/250‑1 (MQ=255)                                                                                                               cTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATcac                                                                        <  1:622183/250‑1 (MQ=255)                                                                                                               cTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCGGACAACCCAAATTCAAGATATAGGAGGATAAGTCAAGGGTAAGTAAAGCCAATGACGATTCAAGTCTATcac                                                                        <  1:898511/250‑1 (MQ=255)                                                                                                                    gCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGAt                                                                                                                   >  1:497472/1‑202 (MQ=255)                                                                                                                    gCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCa                                                                  >  1:37683/1‑251 (MQ=255)                                                                                                                             aaaCTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAg                                                                                                                                                         <  1:355476/155‑1 (MQ=255)                                                                                                                              aaCTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTg                                                        <  1:250955/251‑1 (MQ=255)                                                                                                                                        cTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAg                                                                                                                                                         <  1:741486/144‑1 (MQ=255)                                                                                                                                            tAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTGTAAGTATCACATCg                                          <  1:530057/251‑1 (MQ=255)                                                                                                                                                            gAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTGTAAGTATCACATCGTCTTCACCCCGAAata                          >  1:311444/1‑251 (MQ=255)                                                                                                                                                            gAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTGTAAGTATCACATCGTCTTCACCCCGAAata                          >  1:740269/1‑251 (MQ=255)                                                                                                                                                                  cATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAg                                                                                                                                                         <  1:697418/118‑1 (MQ=255)                                                                                                                                                                  cATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGa                                                                                         <  1:71053/182‑1 (MQ=255)                                                                                                                                                                  cATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTGTAAGTa                                                  <  1:545119/221‑1 (MQ=255)                                                                                                                                                                  cATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTGTAAGTATCACAt                                            <  1:420563/227‑1 (MQ=255)                                                                                                                                                                  cATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTGTAAGTATCACATCGTCTTCAcc                                  <  1:576858/237‑1 (MQ=39)                                                                                                                                                                                    attatAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTGTAAGTATCACATCGTCTTCACCCCGAAATATAGAAGAAAAGCCATTTACGGAAg  >  1:175383/1‑251 (MQ=11)                                                                                                                                                                                                                                                  |                                                                                                                                                                                             ACGTGCTGGTGGCTACACCCGCATCCTTAAATGTGGTTTCCGTGCTGGCGATAATGCTCCAATGGCTTACATTGAGTTAGTAGATCGTCCAGAAGTTTCAAGTAAAGAAGCTACAGCGGAATAAAAACTAGTTAACTGTTAGATTTAAAAAGGCTGAATAACATTTATTCAGCCTTTTTATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAACTTAAGCGGTCAACATAAAAAATCCTCACTATATAAGAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTGTAAGTATCACATCGTCTTCACCCCGAAATATAGAAGAAAAGCCATTTACGGAAG  >  1/688748‑689177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑