Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 587,904 A→G intergenic (+96989/‑108804) unknown → / → unknown tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1587,9040AG100.0% 84.7 / NA 29intergenic (+96989/‑108804)unknown/unknowntRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/13);  total (16/13)

ATACAAAGTATGAGTCGCCGAGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACACACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTAAACTTTACCTGACTGTATTGCAG  >  1/587813‑588149
                                                                                           |                                                                                                                                                                                                                                                     
aTACAAAGTATGAGTCGCCGAGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGAt                                                                                        >  1:579945/1‑251 (MQ=14)
  aCAAAGTATGAGTCGCCGAGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTgg                                                                                                >  1:725956/1‑241 (MQ=14)
     aaGTATGAGTCGCCGAGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTgg                                                                                                <  1:299756/238‑1 (MQ=14)
     aaGTATGAGTCGCCGAGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACtt                                                                                   >  1:140879/1‑251 (MQ=14)
     aaGTATGAGTCGCCGAGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACtt                                                                                   >  1:753827/1‑251 (MQ=14)
     aaGTATGAGTCGCCGAGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACtt                                                                                   >  1:558978/1‑251 (MQ=14)
     aaGTATGAGTCGCCGAGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACtt                                                                                   >  1:535027/1‑251 (MQ=14)
     aaGTATGAGTCGCCGAGGGAATTGCCATGATAATGCGGTGATTGTACGCTTTTTTCCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGCAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGACCCCAATATTTAAGTCAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACtt                                                                                   >  1:383649/1‑251 (MQ=11)
                           tGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTggg                                                                                               <  1:282018/217‑1 (MQ=14)
                           tGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCg                                                             >  1:600070/1‑251 (MQ=255)
                               aTGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAg                                                         <  1:583007/251‑1 (MQ=255)
                                  aTAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGGGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGtt                                                      >  1:434663/1‑251 (MQ=255)
                                  aTAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGt                                                       <  1:628003/250‑1 (MQ=255)
                                        cGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTAcc                                                <  1:772890/251‑1 (MQ=255)
                                        cGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTAc                                                 <  1:232475/250‑1 (MQ=255)
                                                    gCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTAc                                                 >  1:132021/1‑238 (MQ=255)
                                                           tGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACgg                             <  1:773148/251‑1 (MQ=255)
                                                               atatTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTa                         >  1:87863/1‑251 (MQ=255)
                                                                 atTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAg                                                                 >  1:362697/1‑209 (MQ=255)
                                                                 atTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTg                                                               <  1:638822/211‑1 (MQ=255)
                                                                 atTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCg                                               >  1:658604/1‑227 (MQ=255)
                                                                 atTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTaaa                       >  1:600346/1‑251 (MQ=255)
                                                                 atTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTaaa                       >  1:664666/1‑251 (MQ=255)
                                                                   tAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTAAACt                     <  1:155646/251‑1 (MQ=255)
                                                                        tCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTAAACTTTAcc                <  1:880722/251‑1 (MQ=255)
                                                                             gtgtTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTAAACTTTACCTGa             >  1:558036/1‑249 (MQ=255)
                                                                                    tACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTAAACTTTACCTGACTGTATTGc    <  1:19603/251‑1 (MQ=255)
                                                                                    tACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTAAACTTTACCTGACTGTATTGc    <  1:436228/251‑1 (MQ=255)
                                                                                      cTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTAAACTTTACCTGACTGTATTGCAg  <  1:532551/251‑1 (MQ=255)
                                                                                           |                                                                                                                                                                                                                                                     
ATACAAAGTATGAGTCGCCGAGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACACACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAGCTTTTTGGGGGCAGATCACTTTTTGCAGCAAGACAAAAGTGCGGTAGGTTCTTACCGTGTTTTTTACTTCAACGGTCTAAACTTTACCTGACTGTATTGCAG  >  1/587813‑588149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑