Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	4,364	C→T	intergenic (–/+129580)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	4,364	0	C	T	100.0%	161.6 / NA	49	intergenic (–/+129580)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/23);  total (26/23)

TGGGCTTTAGCTTTTTCCAACGCGTTTTCATCCAAATCCACGCCTTCAATGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTCTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAATATTTTATTAAAACTTATCGGTTTTATTACTAAGTTTGGTTATAATGACAAAGGTTTTCTTACATAAATAGGAGCTAAAAATGAAAATCAAAACTATTTTAGCCATAGTAATCGGC  >  1/4117‑4578                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                       tGGGCTTTAGCTTTTTCCAACGCGTTTTCATCCAAATCCACGCCTTCAATGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTttcc                                                                                                                                                                                                                     <  1:602448/251‑1 (MQ=255)   ggCTTTAGCTTTTTCCAACGCGTTTTCATCCAAATCCACGCCTTCAATGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTttcct                                                                                                                                                                                                                    >  1:516603/1‑250 (MQ=255)                tCCAACGCGTTTTCATCCAAATCCACGCCTTCAATGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGGCGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGGCTTCTTCTTTGCCCATTTTGCTTTTTCCTCAATAACACCAAAc                                                                                                                                                                                                      <  1:128082/251‑1 (MQ=255)                         ttttCATCCAAATCCACGCCTTCAATGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACAt                                                                                                                                                                                             >  1:806056/1‑251 (MQ=255)                                 cAAATCCACGCCTTCAATGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGTACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTataaata                                                                                                                                                                                     <  1:456409/251‑1 (MQ=255)                                   aaTCCACGCCTTCAATGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTAtaaataaa                                                                                                                                                                                   <  1:752566/251‑1 (MQ=255)                                           ccTTCAATGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAac                                                                                                                                                                                                             <  1:369366/217‑1 (MQ=255)                                            cTTCAATGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGAtttt                                                                                                                                                                          >  1:460674/1‑251 (MQ=255)                                                 aTGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAg                                                                                                                                                                                                    <  1:704383/220‑1 (MQ=255)                                                 aTGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCtt                                                                                                                                                                                                 <  1:517835/223‑1 (MQ=255)                                                  tgtgGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTaa                                                                                                                                                                    <  1:814766/251‑1 (MQ=255)                                                            gTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAg                                                                                                                                                          >  1:100898/1‑251 (MQ=255)                                                                tGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATta                                                                                                                                                                                           >  1:441275/1‑214 (MQ=255)                                                                                       ggTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATGACACCAAACAGCTTACATTATAaataaata                                                                                                                                                                                 >  1:554303/1‑201 (MQ=255)                                                                                       ggTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTaaa                                                                                                                                             >  1:316196/1‑237 (MQ=255)                                                                                       ggTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAAt                                                                                                                                           >  1:165324/1‑239 (MQ=255)                                                                                        gTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTttcc                                                                                                                                                                                                                     <  1:289996/164‑1 (MQ=255)                                                                                        gTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTttcc                                                                                                                                                                                                                     <  1:699059/164‑1 (MQ=255)                                                                                          acacATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAAt                                                                                                                            <  1:878681/251‑1 (MQ=255)                                                                                            acaTATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAg                                                                                                                          >  1:313349/1‑251 (MQ=255)                                                                                             caTATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACAtt                                                                                                                                                                                            <  1:683782/184‑1 (MQ=255)                                                                                             caTATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACAt                                                                                                                                                                                             >  1:559968/1‑183 (MQ=255)                                                                                             caTATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAAt                                                                                                                                           <  1:503225/233‑1 (MQ=255)                                                                                             caTATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAAt                                                                                                                                           >  1:451004/1‑233 (MQ=255)                                                                                             caTATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAAt                                                                                                                                           >  1:259333/1‑233 (MQ=255)                                                                                             caTATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGt                                                                                                                         >  1:486485/1‑251 (MQ=255)                                                                                               tatTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAAt                                                                                                                                           <  1:391829/231‑1 (MQ=255)                                                                                                atTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAacac                                                                                                                                                                                                           >  1:290212/1‑166 (MQ=255)                                                                                                 ttACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAAc                                                                                                                                                                                                      >  1:243890/1‑170 (MQ=255)                                                                                                 ttACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGAttt                                                                                                                                                                           <  1:660810/197‑1 (MQ=255)                                                                                                 ttACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAat                                                                                                                     >  1:758653/1‑251 (MQ=255)                                                                                                   aCATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAatat                                                                                                                   <  1:706280/251‑1 (MQ=255)                                                                                                    cATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAATAtt                                                                                                                  <  1:793377/251‑1 (MQ=255)                                                                                                    cATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAATAtt                                                                                                                  <  1:353744/251‑1 (MQ=255)                                                                                                            ttCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAacac                                                                                                                                                                                                           <  1:776118/154‑1 (MQ=255)                                                                                                                      tttttATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACAtt                                                                                                                                                                                            <  1:53847/159‑1 (MQ=255)                                                                                                                                    aaaaGCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAAt                                                                                                                                           <  1:895378/194‑1 (MQ=255)                                                                                                                                     aaaTCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTAc                                                                                                                                                                                               <  1:761017/141‑1 (MQ=255)                                                                                                                                      aaTCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGcc                                                                                                                                                        >  1:685351/1‑179 (MQ=255)                                                                                                                                                ggCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAAc                                                                                                                                                                                                      >  1:46768/1‑123 (MQ=255)                                                                                                                                                ggCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAatat                                                                                                                   <  1:264553/206‑1 (MQ=255)                                                                                                                                                            aTCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAATATTTTATTAAAACTTATCGGTTTTATTACTAAGTTTGGTTATAATGACAAAGGtttt                                                            >  1:437958/1‑249 (MQ=255)                                                                                                                                                                   gCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATta                                                                                                                                                                                           >  1:302466/1‑115 (MQ=255)                                                                                                                                                                    cTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGAttt                                                                                                                                                                           <  1:259822/130‑1 (MQ=255)                                                                                                                                                                    cTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAAt                                                                                                                            >  1:682398/1‑177 (MQ=255)                                                                                                                                                                    cTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAatat                                                                                                                   >  1:420696/1‑186 (MQ=255)                                                                                                                                                                    cTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAATATTTTATTAAAACTTATCGGTTTTATTACTAAGTTTGGTTATAATGACAAAGGTTTTCTTACATaaa                                                  >  1:512161/1‑251 (MQ=255)                                                                                                                                                                              gCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAATATTTTATTAAAACTTATCGGTTTTATTACTAAGTTTGGTTATAATGACAAAGGTTTTCTTACATAAATAGGAGCTaa                                        >  1:5762/1‑251 (MQ=255)                                                                                                                                                                                       tAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTataaa                                                                                                                                                                                       >  1:83780/1‑99 (MQ=255)                                                                                                                                                                                             ggTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTataaat                                                                                                                                                                                      <  1:512257/94‑1 (MQ=255)                                                                                                                                                                                                   aCCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAATATTTTATTAAAACTTATCGGTTTTATTACTAAGTTTGGTTATAATGACAAAGGTTTTCTTACATAAATAGGAGCTAAAAATGAAAATCAAAACTAttt                   >  1:709407/1‑251 (MQ=255)                                                                                                                                                                                                                    aaTTATGGCCGACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAATATTTTATTAAAACTTATCGGTTTTATTACTAAGTTTGGTTATAATGACAAAGGTTTTCTTACATAAATAGGAGCTAAAAATGAAAATCAAAACTATTTTAGCCATAGTAATCGGc  <  1:844062/251‑1 (MQ=255)                                                                                                                                                                                                                              gACTTCTTCTTTACCCATTTTGCTTTTTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAATATTTTATTAAAAc                                                                                                        >  1:834023/1‑139 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                       TGGGCTTTAGCTTTTTCCAACGCGTTTTCATCCAAATCCACGCCTTCAATGTGGCAACCGTATTGCTTCGCCAGCCCAATTGCCGTGGTACACATATTACATGCCACTTCCAGCACCTTTTTATCTTGACTAAAATCACCACTGGCAATCAACCAATCCGTCGCTTTACGACCGCCCGGACGTAAACGGGTTTTACCTAAACGCGCTAAAAAATTATGGCCGACTTCTTCTTTACCCATTTTGCTTTCTCCTCAATAACACCAAACAGCTTACATTATAAATAAATATGATTTTTATTAAATATAAAAAGCCAACACATTAAAATAGTAAATGATTTAATAGTTAATATTTTATTAAAACTTATCGGTTTTATTACTAAGTTTGGTTATAATGACAAAGGTTTTCTTACATAAATAGGAGCTAAAAATGAAAATCAAAACTATTTTAGCCATAGTAATCGGC  >  1/4117‑4578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑