Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,138,106	0	C	A	37.7%	105.9 / 27.3	69	intergenic (‑18161/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base C (30/13);  new base A (0/26);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 1.22e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGAAAAAG  >  1/2137857‑2138338                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGcaaca                                                                                                                                                                                                                                          >  1:128569/1‑251 (MQ=255) gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGcaaca                                                                                                                                                                                                                                          >  1:30472/1‑251 (MQ=255) gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGcaaca                                                                                                                                                                                                                                          >  1:160405/1‑251 (MQ=255) gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGcaaca                                                                                                                                                                                                                                          >  1:245522/1‑251 (MQ=255) gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCCCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGcaaca                                                                                                                                                                                                                                          >  1:227188/1‑251 (MQ=255)     ataAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAAc                                                                                                                                                                                                                                      <  1:642865/251‑1 (MQ=255)          cGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑c                                                                                                                                                                                                                                <  1:830379/251‑1 (MQ=255)                aaaGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGcc                                                                                                                                                                                                                          <  1:880470/251‑1 (MQ=255)                              aTCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATc                                                                                                                                                                                                                    >  1:78630/1‑243 (MQ=255)                                  aaccaatttttggcccGCCAAAAAAGCCGATCACATTGCCGAAAAAAGCCCCGCCCCCAACAAACGCAAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCAAACCCGTAAGCGGCATCCCGGCGGCGTTTCCCATGGCAAACAAC‑CCGATAGCGGAGAAAAACAGCAAAAAT‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGcc                                                                                                                                                                                                        <  1:445954/236‑1 (MQ=255)                                  aaaCGATATTTTGCTCGCCAAAAAACCCGATCACATGGCCAAACAAAGTCACGCCCACCAAAAACGCCAGAGACCCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGATTCTGGCGCTGCCGGGTGCTCGCCAGCAAATCCGTAATCGGCATCCCGGCGGCTTTTACCATGGCAAACAAC‑CCGATAGCGGAGAAAAACAGCAAAAAA‑TTCTCGGCAACAAACCGGAG‑CCCCGCCATAATCAAGGCACTTGcc                                                                                                                                                                                                        <  1:515057/251‑1 (MQ=255)                                  aaaCGATATTTTGCTCGCAAAAAAAGCCGATCACATTGCCAAAAAAAGTCACCCCCACCACAACCGCAGGAGAACCG‑TTAACCCGCGCTTGCCCGTAATCCAGTGGAATCGGGCGCTCCCAGTTGCTCGCCAGCGAATCCGTAATCGGCATACCGGGGGCGTTTACCATGGCAAACAAA‑CCAATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGcc                                                                                                                                                                                                        <  1:149963/251‑1 (MQ=255)                                       atatTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAgcg                                                                                                                                                                                                    >  1:614238/1‑250 (MQ=255)                                         atTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCa                                                                                                                                                                                                 >  1:345842/1‑251 (MQ=255)                                            ttGCCCGCCAAAAAACCCGACCCCATGGCCAAAAAAAGTCCCCCCCACCACAAACGCCAGAGAACCGATTAC‑CCGCGCTTTGCCGTAACCCAGTTGAATCGGGCGCTGCCAGGTGCTCCCCAGCGAATCCGTAATCGGCATCCCGGCGGCGTTTACCAGGGCAAACAAA‑CCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATa                                                                                                                                                                                              <  1:83514/251‑1 (MQ=255)                                              ccccGCCAAAAAACCCGACCACATTGCCAAAAAAAGCCACGCCCACCACAAACGCAAGAGACCCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGAATCGGGCGCTGCCGGGTGCCCGCCAGCAAATCCGTAATCGGCATCCCGGGGGCGTTTACCATGGAAAACAAC‑CCGATAGCGGAAAATAACACCCAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAg                                                                                                                                                                                            <  1:881681/248‑1 (MQ=255)                                              ccTCGCCGAAAAAGCCGATCACATTGCCAAACAAAGCCCCGCCCACCACAACCGCCGGAAAACCGTTTAC‑CCGCGCTTGCCCGTAACCCAGTTAAATCGGGCGCGGCCAGGTGCCCGCAAGCAAATCCGAAAGGGGAAACCCGGCGGCTTTTCCCATGGCAAACAAC‑CCAATGGCGGAGAAAACCAGCAAAAAA‑TTCTCGGCAAAAAAACTGGC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATaa                                                                                                                                                                                             <  1:257653/249‑1 (MQ=255)                                                     aaaaaaGCCGATCACATTGCCAAACAAAGTCCCGCCCACCACAAACGCCAGAGACCCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATCCCGGCGGCTTTTACCATGGAAAACAAC‑CCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAgg                                                                                                                                                                                     <  1:373401/250‑1 (MQ=255)                                                      aaaaaGCGGATCCCATTCCCAAACAAGGCCCCGCCCACAACAACCGCAAGAGACCCG‑TTAACCCGCGTTTTGCCGTAATCCGTTTGAATCTGGCGCTGCCGGGTGCTCGCCAGCAAACCCGTAATGGGCATCCGGGGGGCTTTTACCATGGCAAACAAA‑CCGAAAGCGGAAAAAAACAGCAAAAAA‑TTCTCGGCAAAAAAACGGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGa                                                                                                                                                                                    <  1:282450/251‑1 (MQ=255)                                                           cccaatcccaTTGCCAAAAAAAGCCCCGCCCACAACAACCGCCAGACAACCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGAACCGGGCGTGGCCGGGTGCTCGCCGGCGAACCCGTAATGGGCATCCCGGCGGTTTTTACCAGGGCAAACAAA‑CCGATAGGGGAAAATAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTaa                                                                                                                                                                               <  1:417247/243‑1 (MQ=255)                                                             cgaccccatgcccaaaCAAAGCCACCCCCCCCAAAACCGCCAGAAACCCAATTAC‑CCCCCCTTTCCCGTAACCCAGTTGAATCTGGCGCTGCCAGGTCCTCGCCAGCAAACCCGTAACCGCCATCCCGGCGGCGTTTCCCATGCCAAACAAC‑CCGATAGCGGAAAATAACACCCAAAAA‑TTCTCGCCAAAAAAACGGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc                                                                                                                                                                             <  1:508649/237‑1 (MQ=255)                                                             caaTCACATTGCCGAACAAACTCCCGCCCACCACAACCGCCAGAGACCCGATTAC‑CCGCGCTTTGCCGTAACCCAGTTGAATCTGGCGCTGCCGGGTGCCCCCCAGCGAACCCGTAATCGGCATCCCGGCGGCTTTTCCCATGGCAAACAAC‑CCGATAGCGGAAAATAACAGCCAAAAA‑TTCTCGGCAAAAAATCTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc                                                                                                                                                                             <  1:57739/249‑1 (MQ=255)                                                               aTCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑ccc                                                                                                                                                                                                                              >  1:848256/1‑200 (MQ=255)                                                               aTCACATTGCCAAAAAAAGCCCCGCCCACCACAACCGCCAGAGAACCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGAATCGGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCa                                                                                                                                                                            <  1:173510/250‑1 (MQ=255)                                                                ccacaTGGCCGAACAAAGTCACCCCCACCAAAAACGCAAGAGAACCGATAAC‑CCGCGCTTTGCCGAAATCAAGTGAAATCTGGCGCTGCAAGGTGCTCGCCGGCAAATCCGTAACCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCAATGGCGGAAAATAACAGCAAAAAA‑TTCTCGGCAACAAACCTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGc                                                                                                                                                                          <  1:812538/250‑1 (MQ=255)                                                                   caTTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTg                                                                                                                                                                        >  1:507127/1‑250 (MQ=255)                                                                     ttGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGa                                                                                                                                                                     >  1:575492/1‑251 (MQ=255)                                                                       cccaaaCAAAGTCCCCCCCACCACAACCGCCAGAGACCCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATCCCGGCGGCTTTTCCCATGGCAAACAAC‑CCGATAGCGGAAAATAACAGCCAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACg                                                                                                                                                                   <  1:505009/247‑1 (MQ=255)                                                                                  ccACGCCCACCACAAACGCAGGAGACCCGATCAG‑CCGCGTTTTGCCGTAATCCAGTGGATTCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAACCGGAATACGGGCGGCGTTTACCATGGCAAACAAC‑CCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGAAACAAAACGGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTttaatt                                                                                                                                                        <  1:549830/250‑1 (MQ=255)                                                                                           ccacAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGgag                                                                                                                                               >  1:353861/1‑251 (MQ=255)                                                                                                 ccGCCAGAAACCCGATTAC‑CCGCGCTTTGCCGTAATCCGGTGGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAACCCTTAACCGGCATCCCGGCGGCTTTTACCATGGAAAACAAC‑CCGATAGCGGAAAATAACAGCAAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAAc                                                                                                                                         <  1:595519/250‑1 (MQ=255)                                                                                                  cGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTttaatt                                                                                                                                                        >  1:295146/1‑235 (MQ=255)                                                                                                    ccAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAAc                                                                                                                                                                                                                                      <  1:456637/156‑1 (MQ=255)                                                                                                              gATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACaa                                                                                                                            >  1:886135/1‑251 (MQ=255)                                                                                                              gATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACaa                                                                                                                            >  1:480204/1‑251 (MQ=255)                                                                                                                     ccGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGa                                                                                                                      >  1:199485/1‑251 (MQ=255)                                                                                                                               ccGTAATCCAGTGGATTCGGGCGCTGCCAGGTGCTCGCAAGCGAATCCGTAATCGGCATACGGGGGGCTTTTCCCAGGGAAAAAAAA‑CCGAAAGGGGAAAAAAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAg                                                                                                             <  1:823526/250‑1 (MQ=255)                                                                                                                                          ttGAATCGGGCGCTGCCGGGTCCCCCCCAGCAAACCCGAAACCGCCATCCCGGCGGCTTTCCCCATGGAAAACAAC‑CCGATAGCGGAAAAAAACAGCAAAAAA‑TTCTCGGAAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAGTTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGc                                                                                                  <  1:775928/250‑1 (MQ=255)                                                                                                                                          tggAATCTGGCGCTGCCAGGTGCTCGCCAGCGAACCCGTAACCGGCATACCGGCGGCTTTTACCAGGGCAAAAAAC‑CCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGCAACAAAACTGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGc                                                                                                  <  1:78334/248‑1 (MQ=255)                                                                                                                                           tGAATCGGGCGCTGCCGGGTGCTCGCCAGCGAATCCGTAATCGGCATCCCGGCGGCTTTTACCATGGAAAAAAAA‑CCGATAGCGGAAAAAAACAGCCAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                <  1:716253/251‑1 (MQ=255)                                                                                                                                           tGAATCGGGCGCTGCCGGGTGCTCGCCAGCAAATCCGAAATCGGCATACCGGGGGCGTTTACCAGGGCAAAAAAA‑CCGAAAGCGGAAAAAAACAGCCAAAAT‑TTCTCGGCAACAAAACGGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                <  1:361923/251‑1 (MQ=255)                                                                                                                                            gaaccgggcgctcccggggccccGCCAGCGAACCCGTAACCGGCATACGGGCGGCTTTTACCATGGAAAACAAC‑CCGATAGCGAAAAAAAACACCAAAAAA‑TTCTCGGCAAAAAACCGGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                <  1:580713/228‑1 (MQ=255)                                                                                                                                            aaatcgggcgcgcccgggggcccGCCAGCGAACCGGAAAGCGGCACACCGGGGGCGTTTACCA‑GGAAAAAAAACCCGATAGGGGAAAATAACAGCAAAAAA‑TTCTGGGAAAAAAAACGGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                <  1:404887/228‑1 (MQ=255)                                                                                                                                             aaTCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATc                                                                                                                                                                                                                    >  1:678747/1‑133 (MQ=255)                                                                                                                                                cgggCGCTGCAGGGTGCTCGCCAGCAAACCCGTAATCGGCATCCCGGCGGCTTTTACCATGGAAAAAAAC‑CCGATAGCGGAGAATAACAGCCAAAAT‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGc                                                                                           <  1:113154/249‑1 (MQ=255)                                                                                                                                                      cTGCCGGGTGCTCGCCAGCGACTCCGTAATCGGCATCCCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGGGGAAAATAACAGCCAAAAA‑TTCTCGGAAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGa                                                                                                                      <  1:130269/218‑1 (MQ=255)                                                                                                                                                                            tCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAgcgc                                                                                                                                                                                         >  1:128765/1‑129 (MQ=255)                                                                                                                                                                            tCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCAc                                                                                                          >  1:336199/1‑208 (MQ=255)                                                                                                                                                                              cGTAATCGGCATCCCGGCGGCTTTTCCCATGGCAAACAAC‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc                                                                                                                                                                             <  1:217749/139‑1 (MQ=255)                                                                                                                                                                               gTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                >  1:60633/1‑215 (MQ=255)                                                                                                                                                                               gTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGAttt                                                            >  1:285057/1‑251 (MQ=255)                                                                                                                                                                                    cGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTGGGGATTTCATc                                                        >  1:653573/1‑250 (MQ=255)                                                                                                                                                                                      gCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCaa                                                     >  1:890094/1‑251 (MQ=255)                                                                                                                                                                                      gCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCaa                                                     >  1:297192/1‑251 (MQ=255)                                                                                                                                                                                        aTACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCaaa                                                    >  1:817775/1‑250 (MQ=255)                                                                                                                                                                                                 gcttttACCATGGCAAACAAC‑CCGATAGCGGAAAATAACAGCAAAAAA‑TTCTCGGCAAAAAACCTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATa                                                                                                               <  1:584144/179‑1 (MQ=255)                                                                                                                                                                                                 gcgTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc                                                                                                                                                                             >  1:314947/1‑120 (MQ=255)                                                                                                                                                                                                  cttttcccaggaaaaacaaa‑cCGATAGCGGAGAAAAACACCCAAAAT‑TTCTCGAAAACAAAACTGCG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAAc                                         <  1:58037/238‑1 (MQ=255)                                                                                                                                                                                                  cttttACCAGGGAAAACAAA‑CCAATAGAGGAAAAAAACA‑CCAAAAATTTCTCGAAAAAAAAACTGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAAc                                         <  1:738345/249‑1 (MQ=255)                                                                                                                                                                                                     ttaccaggaaaaacaac‑cCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGAAACAAAACGGGG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCa                                                                                                           <  1:649769/172‑1 (MQ=255)                                                                                                                                                                                                          atgaaaaacaac‑cCGATAGCGGAAAAAAACAGCAAAAAA‑TTCTC‑GAAAAAAAACCGGGCCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGaa                                 <  1:820685/246‑1 (MQ=255)                                                                                                                                                                                                          aTGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGaa                                 >  1:314164/1‑251 (MQ=255)                                                                                                                                                                                                             gCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATa                               >  1:259002/1‑250 (MQ=255)                                                                                                                                                                                                                  aaaacccGAAAGCGGAAAAAAACACCCAAAAA‑TTCTCGCCAAAAAACCTGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCa                          <  1:386832/246‑1 (MQ=255)                                                                                                                                                                                                                     a‑cCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAg                      >  1:152252/1‑251 (MQ=255)                                                                                                                                                                                                                       ccGAAAGCGGAGAAAAACACCCAAAAA‑TTCTCGGCAAAAAAACTGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAgc                     <  1:894118/251‑1 (MQ=255)                                                                                                                                                                                                                        cGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAgcg                    >  1:830006/1‑251 (MQ=255)                                                                                                                                                                                                                        cGATAGCGGAAAAAAACAGCAAAAAA‑TTCTCGGAAAAAAACCGGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAgcg                    <  1:791016/251‑1 (MQ=255)                                                                                                                                                                                                                             gCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAgcg                    >  1:665074/1‑246 (MQ=255)                                                                                                                                                                                                                               ggAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCagtagt             >  1:103334/1‑251 (MQ=255)                                                                                                                                                                                                                                gagaATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCagtag              >  1:441594/1‑249 (MQ=255)                                                                                                                                                                                                                                  gaATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATCTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGtagtag           >  1:853748/1‑250 (MQ=255)                                                                                                                                                                                                                                        aaccaaaaaa‑TTCTCGGCAAAAAAACGGGG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCGGCGACGCCCGTTTAATAAAACCGGAGAAAAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGAACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGaaaa    <  1:811688/246‑1 (MQ=255)                                                                                                                                                                                                                                          gCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGAAAAAg  >  1:253708/1‑251 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          GCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGAAAAAG  >  1/2137857‑2138338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑