Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,942,428	0	C	G	44.8%	21.9 / 12.7	30	intergenic (+93052/‑57564)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base C (12/4);  new base G (8/5);  total (21/9)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTG‑TG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGC‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGTGATTGATTGCTTCAAAAAATTGCTGATTTGCGACTACGAGGATTATCAACAATTTGT  >  1/1942190‑1942669                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     gTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTG‑TG‑GCCG‑CCC‑GCATTTTC‑G‑TTT‑TGGTAATgggcgggggcaggag                                                                                                                                                                                                                                           >  1:44150/1‑242 (MQ=255)  ttATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTGAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTG‑TG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGC‑ACA‑GGAcc                                                                                                                                                                                                                                        <  1:441037/250‑1 (MQ=255)     tCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTG‑TG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGC‑ACA‑GGACCCGcc                                                                                                                                                                                                                                    <  1:259864/251‑1 (MQ=255)                     cccgcaaataattttagggggatgatGTGGGAAGATGA‑GGGGGGCCGTTGGGGTGTGCGTTTGGGGAAATCCCGGGAAGGGGAAGGGGATCCGCGCGTGAGAAAAAT‑CGACACCCGTCGGCATGCAATTAGTATGCCGTTTGGGGGGTAAGTTATGGG‑CTTACATTCCCCCGGGGGGGGGGGTCCTG‑TG‑CCCG‑CCC‑GAATTTCC‑G‑TTG‑GGATAAGGGGGGGGC‑TCA‑GGACCCGCCCCTACAAAAGCAActc                                                                                                                                                                                                                    <  1:100806/231‑1 (MQ=255)                                atTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTG‑TG‑GCCG‑CCCGGAATTTA‑‑G‑TTT‑TGGTAAAGGGGGGGG‑ACA‑GGAcccccccccccaaaaaacaatcttgttgtttgg                                                                                                                                                                                                         >  1:28201/1‑226 (MQ=255)                                atTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTG‑TG‑CCCG‑CCC‑CGATTTTC‑G‑TTT‑TGGTAAATGGCGGGG‑ACA‑GGAcccccccccccaaaaaacaacccgtttctttga                                                                                                                                                                                                         >  1:734981/1‑226 (MQ=255)                                        cgagggtgTCGGAGGATGACGGCGTGCGGTTC‑GGTGTGCGTTGGGGGAAATCCGTGGGACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGACACCCGTCTGCATGCCTTTTGTAGGCCTTTTGCGGGGTAAGTTATTGG‑CTTACTTTCTCCGGTAGGGGGGGTTCCTG‑TG‑CCCG‑CCC‑GATTTTAC‑G‑TGT‑GGATAAGGGGGGGGC‑AGA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGc                                                                                                                                                                                                 <  1:901129/245‑1 (MQ=255)                                             atgTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGCCCTG‑TG‑CCCGCCCC‑GAA‑TTAC‑GCTTT‑GG‑TAAAGGGGGGGC‑CGA‑GGGCCCCCCCCCCCAAAAACAAATCTGTTGCTTAGATGGaaaaaagtaa                                                                                                                                                                                            >  1:483677/1‑244 (MQ=255)                                                               gTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCCG‑TG‑GCCGCCCC‑CAATTT‑C‑G‑TTT‑TGGTAAAAGGGGGGC‑GCA‑GGACCCCCCCCCCCAAAAAAAACACTGTTTCTTTGATGTAAAAAAGTAAGGACggagcctttctctt                                                                                                                                                                          >  1:861987/1‑238 (MQ=255)                                                                gggcggttgggtgggctttggggggAATCCCTGGAGGGGGAGGCGGACCCGCGCCGGAGC‑AAATCCGCCACCCGTCTGCTTCCCTTTAGTTTGCTGTTTGGGGGTTAATTTTTTGG‑CTTCCTTTCCCCCGGGGGGGGGGGTCCTG‑CC‑CCCG‑CCC‑AATTTTCC‑T‑TTT‑GAATAAGGGGGGGGC‑ACA‑GGCCCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGaccac                                                                                                                                                                           <  1:239164/229‑1 (MQ=255)                                                                  ccGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCCG‑TG‑CCCC‑CCC‑CCATTTTCGG‑TTT‑GGTAAATGGG‑GGGC‑ACA‑AGACCCCCCCCCCCAAAAACAACTCTTTTGCTTTGATGTAAAGCAGGAAGGACGGATCATTaccacccca                                                                                                                                                                       >  1:42518/1‑248 (MQ=255)                                                                  ccGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTG‑TG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAAAGGGCGGGC‑ACA‑GGACCCCCCCCCCCAAAAACAAACCTGTTGCTTAGATGTAAAACAGTAAGGACGGATCATGaccacccta                                                                                                                                                                       >  1:513918/1‑248 (MQ=255)                                                                          gtgtGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTG‑TG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGC‑ACA‑g                                                                                                                                                                                                                                            >  1:489523/1‑175 (MQ=255)                                                                          gtgtGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCCG‑TGCCCCC‑CCC‑GAATTT‑C‑G‑TTT‑GGGTAATTGGCGGGG‑ACC‑GGACCCCCCCCCCCAAAAACAACTCTTATTCTTAGATGTAAAGCAGGAAAGACGGATCATGAACACCCTAatctttt                                                                                                                                                                >  1:756094/1‑249 (MQ=255)                                                                              gCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTGAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCCG‑TG‑CCCG‑CCC‑GCATTTTC‑G‑TTT‑TGGTAATGGGGGGGC‑ACA‑AGAACCCCCCCCACAAAAAAAAACCTGTTTCTTTGATGTAAAACAGGAAAGAAGGATCATTAACACCCTAatcttttat                                                                                                                                                              >  1:536486/1‑249 (MQ=255)                                                                              gCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCCG‑TG‑CCCC‑CCC‑CGATTTTC‑G‑TTT‑TGTTTATGGGCGGGC‑ACA‑GGACCCGCCCCCACCAAAACAAATCTGTTTCTTTGATGTAAAACAGGAAGGACGGATCacctgctctttttcacaactcc                                                                                                                                                             >  1:301716/1‑229 (MQ=255)                                                                                gTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTG‑TG‑CCCG‑CCC‑CGATTTAC‑G‑GTT‑TGATAATGGGGGGGC‑ACA‑GGACCCCCCCCCCCCAAAACAACACTGTTGGTTTGATGTAAAAGAGGAAAGACGGAACATTAACACCGCAaacttctatcttt                                                                                                                                                          >  1:742703/1‑250 (MQ=255)                                                                                                                         ggagAAAT‑CGTCACCCGTCTGCATGCAATTAGTAGGCCTTTTTGGGGGT‑AGTTTTTGGTCTTACTTTCCCCGGGGGGGGGGGGTCGTG‑CC‑CCCG‑‑CC‑AAATTTTG‑G‑TTTGGGAT‑AGGGGGGGGC‑AAAGGGCCCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGa                                                                                                                 <  1:210994/247‑1 (MQ=255)                                                                                                                                  cGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCCG‑GG‑GCCC‑CCC‑GAAATTTC‑G‑TTT‑TGCTAAAAGGGGGGC‑GCA‑GGACCCCCCCCCCCAAAACCAACTCCGTTTGTTAGATGTTAAAAAGAAAGAACGTCTCATGACCCCCCCAAAATTTTATCTTCTCCACGAACGC‑CTTTTCGAAAACgaagaaaaaaggcaggggccagcgc                                                                                                         >  1:444691/1‑231 (MQ=255)                                                                                                                                                                       gggTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTT‑TT‑CCCC‑CCC‑CAATTTTA‑G‑TTT‑TGATAAAGGGGGGGG‑GCA‑GGGCCCCCCCCCCCAAAAACAAATCTGTTGTTTTGATGTAAAAGAGGAAAGACGTATCATTACCACCCCCATCTTCTTTCTTCTCAACGAACCC‑TTTTTTGAAAAAGAAGAAAAAATGCAGGGGGAGGGGCCGCAAGTAATGAATTACTCTTTTGCAATCGGGCACCAt                                                                   >  1:125069/1‑251 (MQ=255)                                                                                                                                                                                   gg‑CTTACATTCTCCCGTAGGGGCGGGGCCTGTTG‑CCC‑‑CCC‑GAAATTTC‑G‑TTT‑TGGTAATGGGGGGGG‑ACA‑GGACCCCCCCCCCCCAAAACAAATCTCTTGCTTTGATGTAAAGCCATGAGGGCGGATCATTACCACCCTCATATTCTTTTTTCTTTACAAAAGCTTTTTTCGAAAA‑GAAGAAAAAGTGCCAGAGCAGGCGCAGCAAGGAATGTATTACTCTTTTGCAATAGGTCCCCACGTGGGtgtggtt                                                       >  1:876055/1‑248 (MQ=255)                                                                                                                                                                                    ggtttACTTTCCCCCGTGGGGGCGGGTCCGG‑CC‑CCCG‑CCA‑GATTTTAT‑T‑TTT‑GAAAAAGGGGCGGGC‑ACA‑GGCCCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGtgat                                                        <  1:131283/247‑1 (MQ=255)                                                                                                                                                                                     ttttacttcccccggggggggCGGGTCCTG‑GC‑CCCG‑CCT‑GATTTTAG‑G‑TTT‑GGATAA‑GGGGGGGCAACG‑GGCCCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGtgattg                                                      <  1:799218/234‑1 (MQ=255)                                                                                                                                                                                                        ggCGGGTCCTG‑TG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGC‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAatcttctatct                                                                                                                                                            >  1:54269/1‑131 (MQ=255)                                                                                                                                                                                                            ggTCCTG‑TG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGC‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTActc                                                                                      >  1:272364/1‑196 (MQ=255)                                                                                                                                                                                                             gTCCTG‑TG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGC‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCAtg                                                                  >  1:565525/1‑215 (MQ=255)                                                                                                                                                                                                                                cccattatccaaacgtaaattcgggcgggc‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGTGATTGATTGCTTCAAAAAATTGCTGATTTGCGACTACGAgg                  >  1:633978/23‑251 (MQ=255)                                                                                                                                                                                                                                           ttt‑GGATAATGGGCGGGC‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGTGATTGATTGCTTCAAAAAATTGCTGATTTGCGACTACGAGGa                 >  1:679448/1‑240 (MQ=255)                                                                                                                                                                                                                                               ggATAATGGGCGGGC‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGTGATTGATTGCTTCAAAAAATTGCTGATTTGCGACTACGAGGATTATcaac         >  1:482715/1‑245 (MQ=255)                                                                                                                                                                                                                                                gATAATGGGCGGGC‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGTGATTGATTGCTTCAAAAAATTGCTGATTTGCGACTACGAGGa                 >  1:361607/1‑236 (MQ=255)                                                                                                                                                                                                                                                gATAATGGGCGGGC‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGTGATTGATTGCTTCAAAAAATTGCTGATTTGCGACTACGAGGATTATCAACAATTTGt  >  1:143269/1‑251 (MQ=255)                                                                                                                                                                                                                                                gATAAGGGGCGGGC‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGt                                                                                             <  1:852399/160‑1 (MQ=255)                                                                                                                                                                                                                                                       ggcgggc‑ACG‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGtgat                                                        <  1:28545/190‑1 (MQ=255)                                                                                                                                                                                                                                                       ggcgggc‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGTGATTGATTGCTTCAAAAAATTGCTGATTTGCGACTACGAGGa                 >  1:757883/1‑229 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     GTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTG‑TG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGC‑ACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGC‑TTTGTCGAAAACGAAGAACAAGTGCAGGAGCAGGCGCAGCAAGTAATGTATTACTCTTTGGCAATCGGTCACCATGTGGGTGTGATTGATTGCTTCAAAAAATTGCTGATTTGCGACTACGAGGATTATCAACAATTTGT  >  1/1942190‑1942669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑