Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	789,295	0	G	A	43.8%	43.0 / 22.4	48	intergenic (+54422/+32538)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (5/22);  new base A (21/0);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 3.22e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGT‑AGTTAAAGAACTTGAGGCAATTGGTATTCATAATGTAAAAGAAGTGGTTTATAACCCAAGTTATGAACAACTTTTTGAAGAAGAAACCAAACCGGGCTTGGAAGGTTTCGAAAAAGGCACCGTCACCA  >  1/789046‑789540                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                           gTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GTAA‑TTTTCCTAAAATTAAAAAAAAAAAAAAg                                                                                                                                                                                                                                                            >  1:514587/1‑250 (MQ=255)                        ggTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAata                                                                                                                                                                                                                                              <  1:359184/240‑1 (MQ=255)                        ggTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GAAATTTTACTTAAAAT‑CAATAAAAAAAAAAACAAAG‑TTCAAAAAttttattaaaa                                                                                                                                                                                                                                   >  1:275756/1‑243 (MQ=255)                         gTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GAAA‑TTTTCCTAAAAAACAAAAAAAAAAAAAACaaaactggctctttttaacaatt                                                                                                                                                                                                                                    >  1:470834/1‑229 (MQ=255)                                                 gtaaagtaaaGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TGAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAAAG‑TTCAAATATTTTATCAAACTAAC‑AGTTTTTTGGGAG‑AAAttttt                                                                                                                                                                                                        >  1:699921/1‑250 (MQ=255)                                                 gtaaagtaaaGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GAAA‑TTTACCTAAAATGCAATAAAAAATAAAGAAATG‑TTCAAATATTTGATCAAGCTAAA‑AGTTTTTTGGGAG‑AAAttttt                                                                                                                                                                                                        >  1:335800/1‑250 (MQ=255)                                                      gtaaagCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAata                                                                                                                                                                                                                                              <  1:67454/210‑1 (MQ=255)                                                                  gTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GAAA‑TTTTCCTAAAATGCAATAAAAAATAAAAAAATG‑TTTAAATATTTGATCAAGCTAAC‑AGTTTTTTTGGAG‑AAAATTTTATAGAATGTTGTTCAA‑t                                                                                                                                                                                      >  1:873779/1‑251 (MQ=255)                                                                          gTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTATCTAAAAAGCAATAAAAAAAAAAACAATG‑TTCAAAAATTTTATCAAACTAAC‑AGTTTTTTGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑ttttttaat                                                                                                                                                                              >  1:58320/1‑247 (MQ=255)                                                                          gTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GAAA‑TTTATCTAAAATGCAATAAAAAATAAAAAAATG‑TTCAAATATTTTATCAAGCTAAC‑AATTTTTTGGGAG‑AAAATTTTATAGAATGTTGTTCAA‑tttttta                                                                                                                                                                                >  1:354448/1‑247 (MQ=255)                                                                                  tAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAacac                                                                                                                                                                      <  1:706183/251‑1 (MQ=255)                                                                                  tAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAacac                                                                                                                                                                      <  1:41338/251‑1 (MQ=255)                                                                                    aTTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAAtg                                                                                                                                                                                                <  1:551249/224‑1 (MQ=255)                                                                                         aTGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAAAGCAATAAAAAAAAAAACAAAG‑TTTAAATATTTTATCAAGCTAAC‑AAGTTTTAGGGAG‑AAAATTTTATAGAATGTTGTCCCA‑TTTTTAACTTTTAACACAAAGAgg                                                                                                                                                               >  1:525690/1‑251 (MQ=255)                                                                                            cTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGa                                                                                                                                                            <  1:179922/251‑1 (MQ=255)                                                                                                      cGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                                                                                                                                                  <  1:156300/251‑1 (MQ=255)                                                                                                      cGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                                                                                                                                                  <  1:619885/251‑1 (MQ=255)                                                                                                      cGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                                                                                                                                                  <  1:583014/251‑1 (MQ=255)                                                                                                      cGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                                                                                                                                                  <  1:781274/251‑1 (MQ=255)                                                                                                       gggTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GAAA‑TTTATCTAAAATGCAATAAAAAATAAAAAAATG‑TTCAAATATTTGATTAAGCTAAC‑AGttttttt                                                                                                                                                                                                                     >  1:88852/1‑183 (MQ=255)                                                                                                         gtggtTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAg                                                                                                                                                <  1:167907/250‑1 (MQ=255)                                                                                                            gtTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GCAA‑TTTTTCTTAAATGTAATAAAAAAAAAAAAAAAG‑GTCAAAAATTTTATTAAGGTAAA‑AGATTTTAGGGAG‑AAAATTTTATAGAATGTTTTTCAA‑TTTTTTAATTTTTACAAAATGAGGGGAAGTATATGaaaaatat                                                                                                                                            >  1:399784/1‑244 (MQ=255)                                                                                                                aTCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GAAA‑ATTACCCAAAATGCAATAAAAAATAAAACAATG‑TTCAAAAATTTTATCAAGACAACAAGTTTTT‑GGGAG‑AAAATTTTATAAAATGTTTTTCAA‑TTTTTTAATTTTAACACAAAGAGGGGAAGTATATGACAAAAGTaaaa                                                                                                                                        >  1:874142/1‑250 (MQ=255)                                                                                                                 tCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GAAA‑TTTTACTAAAATGCAATAAAAAAAAAAACAAAG‑TTTAAAAATTTTATTAAGATAAA‑AGGTTTTAg                                                                                                                                                                                                                     >  1:768868/1‑175 (MQ=255)                                                                                                                        gtTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCt                                                                                                                                                                                                                                  <  1:738812/156‑1 (MQ=255)                                                                                                                           aCTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAacac                                                                                                                                                                      <  1:753275/210‑1 (MQ=255)                                                                                                                           aCTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAAAAAAACAATG‑TTCAAAAATTTTATCAAACTAAA‑AGTTTTTTGGGAG‑AAAATTTTATAAAAAGTTGTCCAATTTTTTAATTTTTAA‑AAAATGAGGGGAAGTATATGAAAGATGTAAAAAACGG‑AGTTaa                                                                                                                            >  1:247489/1‑251 (MQ=255)                                                                                                                                gAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                                                                                                                                                  <  1:185701/225‑1 (MQ=255)                                                                                                                                         cTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAAATAAGCAATG‑TTCAAATATTTTATCAAGCTAAC‑AGTTTTTATGGAG‑AAAATTTTATAGAATGTTGTTCAATTTTTTAATTTTTAA‑ACAATGAGGTGAAGTATATGAAAGATGTAAACGACGT‑AGTTAAAAAACTTGAGGCaa                                                                                                              >  1:438781/1‑251 (MQ=255)                                                                                                                                             aTCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGGTTAAA‑TTT‑CCTCAAATGCAATAAAAAATAAAACAATG‑TTTAAATATTTTATCAAGCTAAC‑AAATTTTAGGGAG‑AAAATTTCATAGAATGTTTTCCAA‑TTTTTTACTTTTTAAACAATGAGGTGAAGTATATGACAGATGTAAACGAAGT‑AGTTAAAGAAATTGAGGCAAtttg                                                                                                          >  1:436743/1‑249 (MQ=255)                                                                                                                                             aTCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGT‑AGTTAAAGaa                                                                                                                        <  1:2319/237‑1 (MQ=255)                                                                                                                                               cATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GAAA‑TTTTCCTAAAAAGCAAAAAAAAAAAAAAAAAAG‑TTCAAAAATTTTATCAAACTAAA‑AGGTTTTAGGGAG‑AAAATTTTATAGAAAGTTGTTCAA‑TTTTTTAATTTTTacac                                                                                                                                                                      >  1:376196/1‑190 (MQ=255)                                                                                                                                                       cccATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTTCCTAAAATGCAATAAAAAATAAAACAATG‑TTCAAAAATTTTATCAAGCTAAC‑AGATTTTTGGGAG‑AAAATTTTATAGAATGTTGTCCAA‑TTTTTTACTTTTTAAACAATGAGGGGAAGGATATGAAAGATGTAAAAGACGG‑AGTTAAAAAAATTGAGGGAAATGGTATTTATAAt                                                                                                >  1:820410/1‑251 (MQ=255)                                                                                                                                                         cATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGT‑AGTTAAAGAACTTGAGGCAATTGGTATTCATAATGt                                                                                              <  1:76885/251‑1 (MQ=255)                                                                                                                                                           ttGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑GAAA‑TTTAACTAAAATGCAATAAAAAAAAAAACAATG‑TTCAAAAATTTTATCaaaccaacaa                                                                                                                                                                                                                             >  1:437374/1‑118 (MQ=255)                                                                                                                                                              aaaGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGT‑AGTTAAAGAACTTGAGGCAATTGGTATTCATAATGTAAaag                                                                                         <  1:278001/251‑1 (MQ=255)                                                                                                                                                                  tGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGT‑AGTTAAAGAACTTGAGGCAATTGGTATTCATAATGTAAaagaag                                                                                      <  1:532922/250‑1 (MQ=255)                                                                                                                                                                             tttGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCTGAGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAAGG‑TTCAAATATTTGATCAAGCTAAC‑AGTGTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCTA‑TTTTTAACTTTTAACTCAATGAGGTGAAGTATATGACAGATGTAAACGTCGT‑AGTTAAAGAACTTGAGGCAGTTGGTATTCATAATGTAAAAGAAGTGGTTTATAAcc                                                                          <  1:434220/251‑1 (MQ=255)                                                                                                                                                                             tttGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑ttt                                                                                                                                                                                    <  1:461135/146‑1 (MQ=255)                                                                                                                                                                                     aCTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TGAA‑TTTATCTAAAAAACAAAAAAAAAAAAAAAAATGTTTCAAA‑AATTGATTAAGCCAAC‑AGGTTTTAGGGGG‑AAATTTTTATAGAATGTTGTTCAATTTTTTAATTTTTAA‑ACAAACAGGGGGAGTATATTACAGATGTAAAAAACGTAAATTAAA‑AAATTGAGGTAATTTGTATTCATAATGTAAaagaaaggggttttaaaccaaggttt                                                                  >  1:691657/1‑230 (MQ=255)                                                                                                                                                                                     aCTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTATCTAAAAAGCAATAAAAAAAAAAACAATG‑TTCAAATATTTTATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTTATAGAATGTTGTCCAA‑TTTTTAACTTTTTACACAATGAGGTGAAGTATATGACAGATGTAAAAGACGT‑AGTTAAAGAACTTGAGGCAATTGGGATTTATAATGTAAaagaaaagggttttaaaccaaattat                                                                  >  1:800481/1‑230 (MQ=255)                                                                                                                                                                                     aCTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGAc                                                                                                                                                  <  1:137333/172‑1 (MQ=255)                                                                                                                                                                                     aCTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑ATTACCTAAAATGCAAAAAAAAAAAAAACAAAG‑TTTAAAAATTTGATCAAGCTAAA‑AGTTTTTAg                                                                                                                                                                                                                     >  1:825090/1‑107 (MQ=255)                                                                                                                                                                                      cTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTAACTTAAATGCAATAAAAAAAAAAACAATG‑TTCCAATATTTGTTCTAGCTAAC‑AGATTTTTGGGAG‑AAATTTTCATTGAATTTTGTCCAA‑TTTTTAACTTTTAACACAATTAGGGGAAGTatattac                                                                                                                                                  >  1:351615/1‑168 (MQ=255)                                                                                                                                                                                      cTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑ATTACCTAAAATGCAATAAAAAAAAAAACAAAGTTTCAAA‑ATTTTATCAAGCTAAA‑AGTTTTTAGGGAGAAAATTTTC‑TAGAATATTGTCCAA‑TTTTTAAATTTTAACACAAAGAGGGGAAGAATATGACAGATGTAAAAGAAGT‑AGTTAAAGAAATTTAGGGAATTGGGATTCATAATATAAAAAAAGGGGGTtatctttctcttataa                                                                 >  1:612188/1‑238 (MQ=255)                                                                                                                                                                                                 gCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTTCCTAAAAAGCAATAAAAAATAAAACAATT‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTTGGGAG‑AAATTTTCATAGAATTTTTTCCAA‑TTTTTAACTTTTTACACAATGAGGTGAAGTATATGACAGATGTAAACGACGT‑AGTTTAAGAACATGAGGCAATTGGTATTCATAATGTAAAAGAAGTGGGTTATAAACCAAGATATGAAAAACttttt                                                      >  1:4065/1‑251 (MQ=255)                                                                                                                                                                                                 gCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGT‑AGTTAAAGAACTTGAGGCAATTGGTATTCATAATGTAAaagaag                                                                                      <  1:578563/219‑1 (MQ=255)                                                                                                                                                                                                                                                       aTAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGT‑AGTTAAAGAACTTGAGGCAATTGGTATTCATAATGTAAAAGAAGTGGTTtat                                                                              >  1:891784/1‑175 (MQ=255)                                                                                                                                                                                                                                                       aTAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGT‑AGTTAAAGAACTTGAGGCAATTGGTATTCATAATGTAAAAGAAGTGGTTTATAACCCAAGTTATGAACAACTTTTTGAAGAAGAAACCAAACCGGGCTTGGAAGGTTTCGAAAAAGGCACCGTcacca  >  1:231110/1‑251 (MQ=255)                                                                                                                                                                                                                                                            gCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTatat                                                                                                                                                     <  1:356183/100‑1 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                           GTATTTGATGTTTAAACCGGCAAGGTCTGCAGCCCACGCGGCGGGAAAGTAAAGTAAAGCGACAAGTAAAAAGGTTTTGCGTAATTTCATGCTATTCCTTCCGGGTGGTTGATCATTGTGTTACTTAGAACGGGCGCTAAATCATAAGTTCCCATTGAAAGTGCGGTGGTTTTTTGCGTTACTTTTAATAGAGCGAAAATGCTTCTCAAATCGGCGGG‑TAAA‑TTTACCTAAAATGCAATAAAAAATAAAGCAATG‑TTCAAATATTTGATCAAGCTAAC‑AGTTTTTAGGGAG‑AAATTTTCATAGAATGTTGTCCAA‑TTTTTAACTTTTAACACAATGAGGTGAAGTATATGACAGATGTAAACGACGT‑AGTTAAAGAACTTGAGGCAATTGGTATTCATAATGTAAAAGAAGTGGTTTATAACCCAAGTTATGAACAACTTTTTGAAGAAGAAACCAAACCGGGCTTGGAAGGTTTCGAAAAAGGCACCGTCACCA  >  1/789046‑789540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑