Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,585,795	(TA)5→4	intergenic (+253273/+16004)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,585,786	0	A	.	100.0%	38.8 / NA	10	intergenic (+253264/+16014)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/1);  total (9/1)
*	1	1,585,787	0	T	.	100.0%	38.8 / NA	10	intergenic (+253265/+16013)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/1);  total (9/1)

TAATCCCAATGTTTCACCACGGCGATGGTGTCGTCGTGCTTTGCCAATACGTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAATATATATATAACGATTGATTATATCGTAATTGTTATCACAGAAATGAAATTAATTTACTGATTTATAAGGAAATAAAGGAATTAATTTATTTTACTTGACTGAAATCAAAGTATTTATATTTAGATACACTAGAATCAACACTAACTCGTTGTAGTTATAAATTCCTTTTAGTTATTAAAACGCTCAAACATCGGAGTGATTCCATGTCAAAAACAAAAAAAATTATAA  >  1/1585538‑1586015                                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                                     tAATCCCAATGTTTCACCACGGCGATGGTGTCGTCGTGCTTTGCCAATACGTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAat                                                                                                                                                                                                                                      <  1:333416/250‑1 (MQ=255)  aaTCCCAATGTTTCACCACGGCGATGGTGTCGTCGTGCTTTGCCAATACGTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCGCGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAatat                                                                                                                                                                                                                                    <  1:55694/251‑1 (MQ=255)      ccAATGTTTCACCACGGCGATGGTGTCGTCGTGCTTTGCCAATACGTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAatatatat                                                                                                                                                                                                                                <  1:869924/251‑1 (MQ=255)        aaTGTTTCACCACGGCGATGGTGTCGTCGTGCTTTGCCAATACGTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAatatatataa                                                                                                                                                                                                                              <  1:661987/251‑2 (MQ=255)                    cGGCGATGGTGTCGTCGTGCTTTGCCAATACGTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATATCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAatatatat                                                                                                                                                                                                                                <  1:741743/237‑1 (MQ=255)                      gCGATGGTGTCGTCGTGCTTTGCCAATACGTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAata                                                                                                                                                                                                                                     <  1:329478/230‑1 (MQ=255)                                               aTACGTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAA‑‑ATATATATAACGATTGATTATATCGTAATTGTTATCACAgaaatgaaat                                                                                                                                                                                     >  1:523327/1‑251 (MQ=255)                                                   gTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAatatatataa                                                                                                                                                                                                                              >  1:184092/1‑207 (MQ=255)                                                   gTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAat                                                                                                                                                                                                                                      <  1:283103/200‑1 (MQ=255)                                                        gAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAA‑‑ATATATATAACGATTGATTATATCg                                                                                                                                                                                                             >  1:834662/1‑218 (MQ=255)                                                                                                                                                  tGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAA‑‑ATATATATAACGATTGATtat                                                                                                                                                                                                                 >  1:356477/1‑124 (MQ=255)                                                                                                                                                     tAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAA‑‑ATATATATAACGATTGATTATATCGTAATTGTTATCACAGAAATGAAATTAATTTACTGATTTATAAGGAAATAAAGGAATTAATTTATTTTACTTGACTGAAATCAAAGTATTTATATTTAGATACACTAGAATCAACACTAACTCGTTg                                                                               >  1:157798/1‑251 (MQ=255)                                                                                                                                                       aTTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAA‑‑ATATATATAACGATTGATTATATCGTAATTGTTATCACAGAAATGAAATTAATTTACTGATTTATAAGGAAATAAAGGAATTAATTTATTTTACTTGACTGAAATCAAAGtatttatat                                                                                                               >  1:94507/1‑217 (MQ=255)                                                                                                                                                              ttttAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAatatatataac                                                                                                                                                                                                                             >  1:505859/1‑100 (MQ=255)                                                                                                                                                              ttttAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAA‑‑ATATATATAACGATTGATTATATCGTAATTGTTATCACAGAAATGAAATTAATTTACTGATTTATAAGGAAATAAAGGAATTAATTTATTTTACTTGACTGAAATCAAAGTATTTATATTTAGa                                                                                                          >  1:387296/1‑215 (MQ=255)                                                                                                                                                                          gAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAatatatataac                                                                                                                                                                                                                             >  1:857053/1‑88 (MQ=255)                                                                                                                                                                                       tATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAA‑‑ATATATATAACGATTGATTATATCGTAATTGTTATCACAGAAATGAAATTAATTTACTGATTTATAAGGAAATAAAGGAATTAATTTATTTTACTTGACTGAAATCAAAGTATTTATATTTAGATACACTAGAATCAACACTAACTCGTTGTAGTTATAAATTCCTTTTAGTTATTAAAACGCt                                              >  1:51444/1‑250 (MQ=255)                                                                                                                                                                                           ttAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAA‑‑ATATATATAACGATTGATTATATCGTAATTGTTATCACAGAAATGAAATTAATTTACTGATTTATAAGGAAATAAAGGAATTAATTTATTTTACTTGACTGAAATCAAAGTATTTATATTTAGATACACTAGAATCAACACTAACTCGTTGTAGTTATAAATTCCTTTTAGTTATTAAAACGCTCaaa                                          >  1:242020/1‑250 (MQ=255)                                                                                                                                                                                                            aaTTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAA‑‑ATATATATAACGATTGATTATATCGTAATTGTTATCACAGAAATGAAATTAATTTACTGATTTATAAGGAAATAAAGGAATTAATTTATTTTACTTGACTGAAATCAAAGTATTTATATTTAGATACACTAGAATCAACACTAACTCGTTGTAGTTATAAATTCCTTTTAGTTATTAAAACGCTCAAACATCGGAGTGATTCCATg                        <  1:884860/251‑1 (MQ=255)                                                                                                                                                                                                                                   tttAAACCCTCTTAATATAAAA‑‑ATATATATAACGATTGATTATATCGTAATTGTTATCACAGAAATGAAATTAATTTACTGATTTATAAGGAAATAAAGGAATTAATTTATTTTACTTGACTGAAATCAAAGTATTTATATTTAGATACACTAGAATCAACACTAACTCGTTGTAGTTATAAATTCCTTTTAGTTATTAAAACGCTCAAACATCGGAGTGATTCCATGTCAAAAACAAAAAAAATTATaa  >  1:323273/1‑250 (MQ=255)                                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                                     TAATCCCAATGTTTCACCACGGCGATGGTGTCGTCGTGCTTTGCCAATACGTTACGAATATTGCCGGTCAGAATTTTCACGAACCGTTTCCGCACGGATTCGCTTTTAATCATAATTTCAGGAAATAATTTGACGATAAATTTCATGTTAATTCACTTTTTAACCCATAGAAAAATGGGGCGTATTTTAGCAGATAGCACAAAAATTACCATTGGCTCAAACTCCTTTTAAACCCTCTTAATATAAAAATATATATATAACGATTGATTATATCGTAATTGTTATCACAGAAATGAAATTAATTTACTGATTTATAAGGAAATAAAGGAATTAATTTATTTTACTTGACTGAAATCAAAGTATTTATATTTAGATACACTAGAATCAACACTAACTCGTTGTAGTTATAAATTCCTTTTAGTTATTAAAACGCTCAAACATCGGAGTGATTCCATGTCAAAAACAAAAAAAATTATAA  >  1/1585538‑1586015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑