Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,365,911	2 bp→GT	coding (361‑362/453 nt)	unknown →	Transposase, IS200 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,365,911	0	A	G	100.0%	11.0 / NA	5	T121V (ACA→GTA)	unknown	Transposase, IS200 family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/2);  total (3/2)
*	1	1,365,912	0	C	T	100.0%	10.2 / NA	5	T121V (ACA→GTA)	unknown	Transposase, IS200 family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/2);  total (3/2)

CGGTAGGGCTAAATACAAAGACAGTGGAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGGATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACC  >  1/1365891‑1366151                     ||                                                                                                                                                                                                                                                cGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGgtg            >  1:901509/1‑251 (MQ=255)   gTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTa          >  1:239399/1‑251 (MQ=255)       ggCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAAttt      >  1:88441/1‑251 (MQ=255)       ggCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAAt        <  1:173696/249‑1 (MQ=255)             aTACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTAcc  <  1:91189/249‑1 (MQ=255)                     ||                                                                                                                                                                                                                                                CGGTAGGGCTAAATACAAAGACAGTGGAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGGATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACC  >  1/1365891‑1366151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑