Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 534,607 C→T S28N (AGC→AAC)  unknown ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1534,6070CT100.0% 108.3 / NA 33S28N (AGC→AAC) unknownhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/16);  total (17/16)

CCGATTTGTTGGGCATAAAGATCAACAGCAACCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGCTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATAAAATTCCTTGATAATCAATATATCGAATTTGACGGCAAAGTAACAAAATTT  >  1/534365‑534842
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           
ccGATTTGTTGGGCATAAAGATCAACAGCAACCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTg                                                                                                                                                                                                                                      <  1:128079/250‑1 (MQ=255)
                   gATCAACAGCAACCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTACATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATa                                                                                                                                                                                                                  >  1:219166/1‑251 (MQ=255)
                    aTCAACAGCAACCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACAt                                                                                                                                                                                                                   <  1:650499/249‑1 (MQ=255)
                             aaCCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGaaa                                                                                                                                                                                                        >  1:22882/1‑251 (MQ=255)
                                      aaGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATc                                                                                                                                                                                               <  1:820335/251‑1 (MQ=255)
                                                 ggTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCaaa                                                                                                                                                                                    >  1:614643/1‑251 (MQ=255)
                                                                   ccGTCTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAAcc                                                                                                                                                                                                                                        >  1:204036/1‑181 (MQ=255)
                                                                               tGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATttaaa                                                                                                                                                      >  1:115403/1‑251 (MQ=255)
                                                                                                    tGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATAc                                                                                                                                 <  1:276290/251‑1 (MQ=255)
                                                                                                      aaaTTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTtatata                                                                                                                                  >  1:100147/1‑248 (MQ=255)
                                                                                                          tattTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATAc                                                                                                                                 >  1:150893/1‑245 (MQ=255)
                                                                                                              ttACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCaa                                                                                                                       <  1:685534/251‑1 (MQ=255)
                                                                                                               tACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCaa                                                                                                                                                                                     <  1:868973/188‑1 (MQ=255)
                                                                                                                    gCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACAt                                                                                                                                                                                                                   >  1:664545/1‑153 (MQ=255)
                                                                                                                                 aCATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTTAATGCTCGCTACCCGTAAAgg                                                                                                    <  1:263276/251‑1 (MQ=255)
                                                                                                                                  cATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCt                                                                                                                                        <  1:589064/214‑1 (MQ=255)
                                                                                                                                               aaaaTCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAAcc                                                                                                                                                                                                                                        >  1:365853/1‑105 (MQ=255)
                                                                                                                                                      aCATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTaa                                                                               >  1:393627/1‑251 (MQ=255)
                                                                                                                                                           gAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAGAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAg                                                                                                                                              >  1:488816/1‑183 (MQ=255)
                                                                                                                                                                ttGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGt                                                                     <  1:3586/251‑1 (MQ=255)
                                                                                                                                                                      atgtgtataaagagacagaaaTCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGcac                                                                <  1:234885/232‑1 (MQ=255)
                                                                                                                                                                              gTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATc                                                                                                                                                                                               <  1:360797/115‑1 (MQ=255)
                                                                                                                                                                                      caAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATaa                                                    >  1:511141/1‑246 (MQ=255)
                                                                                                                                                                                      caAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATaa                                                    >  1:322295/1‑246 (MQ=255)
                                                                                                                                                                                                 ccGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGaa                                                                                                                                            <  1:98056/147‑1 (MQ=255)
                                                                                                                                                                                                                 tctATATTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATaaaa                                                  <  1:826514/221‑1 (MQ=255)
                                                                                                                                                                                                                    atatTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATc                                                                                                                                                                                               <  1:256623/77‑1 (MQ=255)
                                                                                                                                                                                                                    atatTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAg                                                                                                                                                              >  1:42166/1‑110 (MQ=255)
                                                                                                                                                                                                                    atatTTAATTCGCCAAGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAg                                                                                                                                              >  1:77459/1‑126 (MQ=255)
                                                                                                                                                                                                                                   aGATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATAAAATTCCTTGATAATCAATATATCGAATTTGACGGCAAAGTAACAAAAttt  >  1:681000/1‑251 (MQ=255)
                                                                                                                                                                                                                                    gATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAgg                                                                                                    <  1:166635/152‑1 (MQ=255)
                                                                                                                                                                                                                                    gATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGAc                                                                            >  1:820905/1‑176 (MQ=255)
                                                                                                                                                                                                                                    gATTTAACAGGCTGTTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATAAAAt                                                 <  1:554534/203‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           
CCGATTTGTTGGGCATAAAGATCAACAGCAACCCGACCAAGACAAATAAGGTCTAATGTTTTTTCCACCGTTTTCATAATGCAATTCCTTCTTATTTTAATGAAATTATTTTACCTGCATAAAATTGAAACATATATTTCAAAAAAATCAACATTGAAATTTGTTTTTTTAGACGTTAATCACAAAATCAGCCCCGTTAAATTTTCTTTTCTATATTTAATTCGCCAAGATTTAACAGGCTGCTAACCTGATCTTGCAAGATAAAACATAAATTCGGAAAAATTTGATCTATGTCGCAAAAATGAAATTTTTGTTTCAAAAGCATTTAAAATTAAAAGAATGCTTATATACTGGGTTTCAATGCTCGCTACCCGTAAAGGAGATTCTGATGAAAACCGTAAGACTTACTGTTGCACAAGCACTTATAAAATTCCTTGATAATCAATATATCGAATTTGACGGCAAAGTAACAAAATTT  >  1/534365‑534842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑