Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	462,022	C→T	intergenic (‑137/‑81)	unknown ← / → unknown	RNA chaperone ProQ/Inner membrane protein YebS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	462,022	0	C	T	100.0%	101.9 / NA	31	intergenic (‑137/‑81)	unknown/unknown	RNA chaperone ProQ/Inner membrane protein YebS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

CCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGCCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAATGTGTTATATGCCACCAGC  >  1/461798‑462255                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          cccccTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTaaaa                                                                                                                                                                                                                 >  1:881364/1‑251 (MQ=255)    ccTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACg                                                                                                                                                                                                              >  1:157293/1‑251 (MQ=255)              aaaaCATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAg                                                                                                                                                                                                    >  1:809775/1‑251 (MQ=255)                       cGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAAt                                                                                                                                                                                            <  1:293081/250‑1 (MQ=255)                       cGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAAt                                                                                                                                                                                            <  1:604096/250‑1 (MQ=255)                                                       ctTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGAtt                                                                                                                                                            >  1:315192/1‑250 (MQ=255)                                                                                ttCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAAtt                                                                                                                                  <  1:312416/251‑1 (MQ=255)                                                                                          gCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATAc                                                                                                                                                                                                      >  1:374209/1‑173 (MQ=255)                                                                                                         gATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAata                                                                                                                                                                            >  1:313256/1‑184 (MQ=255)                                                                                                              taataaGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCtcgttc                                                                                                    <  1:67662/251‑1 (MQ=255)                                                                                                                    gTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCg                                                                                              >  1:317869/1‑251 (MQ=255)                                                                                                                               tACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGAc                                                                                                                                                      >  1:515963/1‑184 (MQ=255)                                                                                                                                    tAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGt                                                                                                                                                                     <  1:823970/164‑1 (MQ=255)                                                                                                                                      gTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTgg                                                                            >  1:123839/1‑251 (MQ=255)                                                                                                                                       tttAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGaatgcaatgc                                                                                 <  1:877365/245‑1 (MQ=255)                                                                                                                                           gCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCAt                                                                                                              >  1:223171/1‑212 (MQ=255)                                                                                                                                                               aCAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAgg                                                   <  1:655056/251‑1 (MQ=255)                                                                                                                                                                  aTTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGta                                                                                                                                                                                                  >  1:477018/1‑105 (MQ=255)                                                                                                                                                                   ttAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTc                                                                                                                                                                                                                          >  1:351350/1‑80 (MQ=255)                                                                                                                                                                          tctatctaCTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGccc                                        <  1:55563/251‑1 (MQ=255)                                                                                                                                                                               ctaCTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTg                                           >  1:419572/1‑243 (MQ=255)                                                                                                                                                                                            ataataAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGa                      >  1:493482/1‑251 (MQ=255)                                                                                                                                                                                              aataaATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCAt                                                                                                              >  1:64714/1‑161 (MQ=255)                                                                                                                                                                                              aataaATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAAtt                                                                    <  1:408338/203‑1 (MQ=255)                                                                                                                                                                                              aataaATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAAt                    >  1:621714/1‑251 (MQ=255)                                                                                                                                                                                               ataaATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAAtg                   <  1:650315/251‑1 (MQ=255)                                                                                                                                                                                                  aaTTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAATgtgt                <  1:282218/251‑1 (MQ=255)                                                                                                                                                                                                       cgcgAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTGCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAATGTGTTatat           <  1:438433/251‑1 (MQ=255)                                                                                                                                                                                                                      cacTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAATGTGTta              <  1:380613/233‑1 (MQ=255)                                                                                                                                                                                                                          ttttCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAATGTGTTATATGCCACCAGc  >  1:681513/1‑241 (MQ=255)                                                                                                                                                                                                                          ttttCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGAGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGaa                     >  1:661917/1‑222 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          CCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGCCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAATGTGTTATATGCCACCAGC  >  1/461798‑462255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑