Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	6	270	G→T	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	6	270	0	G	T	100.0%	77.9 / NA	24	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/11);  total (13/11)

CTAACTATTTTCCGCTTGCGGAAAATGCAGGGCGAAGGCTTGCAGCCCGGTAAATAATGCTCGATGTTATTCGGGCATTTTTATTTGTTGCGCGCTGATGATTTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATGTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGGCCTGTGAATAACTCTGTGTTTTTATTAATTCGTAGCGTTTGTTAACGAAGTCACTAAATTCGCATAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  6/37‑396                                                                                                                                                                                                                                          |                                                                                                                                                                                                       aactatttttgggcaagctcaaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAgg                                                                                                                                                                                        >  1:346280/22‑250 (MQ=255) aactatttttgagcaagctcaaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGc                                                                                                                                                                                       <  1:236940/230‑1 (MQ=255)                                 cGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAAcc                                                                                                                                                        <  1:367120/250‑1 (MQ=255)                                             gCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACaa                                                                                                                                                                                          <  1:828074/204‑1 (MQ=255)                                                                                               tacctaaatttAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAAt                                                                                                                                    >  1:749381/9‑208 (MQ=255)                                                                                                                       aaaTTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGACTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatatta                                                                 >  1:358946‑M2/1‑242 (MQ=255)                                                                                                                          tttCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatattatgt                                                              >  1:641107‑M2/1‑239 (MQ=255)                                                                                                                                          aTACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatattatgttaaataaaataaatgg                                              >  1:334688‑M2/1‑223 (MQ=255)                                                                                                                                             ccccTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAAc                                                                                                                                 >  1:520655/1‑165 (MQ=255)                                                                                                                                             ccccTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatattatgttaaataaaataaatggtaa                                           <  1:814500‑M2/251‑32 (MQ=255)                                                                                                                                             ccccTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatatt                                                                  >  1:332040‑M2/1‑220 (MQ=255)                                                                                                                                                                 ctgctgATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTc                                                                                         >  1:510357/1‑185 (MQ=255)                                                                                                                                                                   gctgATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatatt                                                                  <  1:591573‑M2/206‑9 (MQ=255)                                                                                                                                                                     tgATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAActc                                                                                                                               <  1:562196/143‑1 (MQ=255)                                                                                                                                                                      gATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatatta                                                                 >  1:882711‑M2/1‑195 (MQ=255)                                                                                                                                                                      gATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatat                                                                   >  1:873395‑M2/1‑195 (MQ=255)                                                                                                                                                                          aGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatat                                                                   <  1:413914‑M2/198‑8 (MQ=255)                                                                                                                                                                               aTGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTgtgt                                                                                                                          >  1:182044/1‑138 (MQ=255)                                                                                                                                                                               aTGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTaaa                                                                                   <  1:613261/177‑1 (MQ=255)                                                                                                                                                                                    aTACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatattatgttaaataaaataaatggtaagtcacttctgggaatttgagatttgctctctttaaaaa     >  1:732398‑M2/1‑181 (MQ=255)                                                                                                                                                                                       cGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatattatgttaaataaaataaatggtaagtcacttctgggaatttgagatttgctctctttaaaaatga  <  1:684168‑M2/250‑73 (MQ=255)                                                                                                                                                                                                                        gTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAAc                                                                                                                                 <  1:324039/90‑1 (MQ=255)                                                                                                                                                                                                                                 aaTCAATATTTATTGATATTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatattatgt                                                              >  1:281235‑M2/1‑136 (MQ=255)                                                                                                                                                                                                                                      atatTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAAcatatattatgt                                                              <  1:796235‑M2/143‑13 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                       CTAACTATTTTCCGCTTGCGGAAAATGCAGGGCGAAGGCTTGCAGCCCGGTAAATAATGCTCGATGTTATTCGGGCATTTTTATTTGTTGCGCGCTGATGATTTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATGTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGGCCTGTGAATAACTCTGTGTTTTTATTAATTCGTAGCGTTTGTTAACGAAGTCACTAAATTCGCATAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  6/37‑396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑