Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	4	316	T→G	V18V (GTT→GTG)	unknown →	Type II secretory pathway, component PulD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	4	316	0	T	G	100.0%	256.0 / NA	74	V18V (GTT→GTG)	unknown	Type II secretory pathway, component PulD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (47/27);  total (47/27)

CCATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGCGCTTATCTTATGCGTTTTGTTATTCGCCGCATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATACCGGGTAGCGGTAAATCTTATGAGGTTGTGTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  4/76‑454                                                                                                                                                                                                                                                 |                                                                                                                                                               ccATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCgg                                                                                                                                                      <  1:836390/251‑1 (MQ=255)                    ttGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTAtct                                                                                                                                   <  1:14607/251‑1 (MQ=255)                     tGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTAtctc                                                                                                                                  <  1:77646/251‑1 (MQ=255)                          gTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTAtctc                                                                                                                                  >  1:432962/1‑246 (MQ=255)                          gTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAgg                                                                                                                             >  1:829483/1‑251 (MQ=255)                           tGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAgg                                                                                                                             <  1:645551/250‑1 (MQ=255)                                 aTTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGc                                                                                                                      <  1:302693/251‑1 (MQ=255)                                         aaaTTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTaa                                                                                                               >  1:131072/1‑250 (MQ=255)                                           aTTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGa                                                                                                            >  1:521528/1‑251 (MQ=255)                                           aTTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGa                                                                                                            >  1:148051/1‑251 (MQ=255)                                           aTTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTCCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGa                                                                                                            >  1:809958/1‑251 (MQ=255)                                               aaCAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAatat                                                                                                        <  1:391185/251‑1 (MQ=255)                                                   gCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAg                                                                                                    >  1:659615/1‑251 (MQ=255)                                                   gCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAg                                                                                                    >  1:85712/1‑251 (MQ=255)                                                           gTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATaa                                                                                             >  1:366247/1‑250 (MQ=255)                                                                gTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAAc                                                                                            >  1:840468/1‑246 (MQ=255)                                                                gTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAg                                                                                          <  1:361202/248‑1 (MQ=255)                                                                gTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACg                                                                                       >  1:760345/1‑251 (MQ=255)                                                                       agaATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAg                                                                                 <  1:685450/250‑1 (MQ=255)                                                                        gaATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTa                                                                               >  1:14092/1‑251 (MQ=255)                                                                          aTGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTATCTTATGCGTTTTGTTATTCGTCGTATTCTGATTATAGGTTGAGGATTTTATGGCAATTTCGGGTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCAATGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAg                                                                             >  1:116770/1‑251 (MQ=255)                                                                              ggCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTa                                                                                                                                      <  1:23995/190‑1 (MQ=255)                                                                              ggCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAg                                                                         >  1:598379/1‑251 (MQ=255)                                                                                   atatTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGAc                                                                                                                        >  1:540681/1‑199 (MQ=255)                                                                                   atatTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTg                                                                    <  1:756469/251‑1 (MQ=255)                                                                                    tatTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGt                                                                   >  1:752941/1‑251 (MQ=255)                                                                                       tttAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTAt                                                                      >  1:51544/1‑245 (MQ=255)                                                                                          aaaaaTGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAAt                                                                                                                                        <  1:127859/176‑1 (MQ=255)                                                                                          aaaaaTGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTg                                                                    >  1:564071/1‑244 (MQ=255)                                                                                                     gcgTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTaa                                                                                                               >  1:444431/1‑190 (MQ=255)                                                                                                     gcgTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           <  1:195520/226‑1 (MQ=255)                                                                                                          gATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAg                                                                                 <  1:657494/215‑1 (MQ=255)                                                                                                          gATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAAGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAgg                                             >  1:444322/1‑251 (MQ=255)                                                                                                                gCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTgag                                                                                                                                           <  1:210130/151‑1 (MQ=255)                                                                                                                gCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGa                                                                                                                            >  1:506073/1‑166 (MQ=255)                                                                                                                gCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAAc                                                                                            <  1:779253/198‑1 (MQ=255)                                                                                                                 cTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAat                                                                                                          >  1:9532/1‑183 (MQ=255)                                                                                                                 cTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGa                                      >  1:820060/1‑251 (MQ=255)                                                                                                                 cTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGa                                      >  1:181379/1‑251 (MQ=255)                                                                                                                          tggtTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGc                              <  1:684602/250‑1 (MQ=255)                                                                                                                          tggtTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGc                              <  1:5837/250‑1 (MQ=255)                                                                                                                              tttAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGttt                                                                 <  1:234879/211‑1 (MQ=255)                                                                                                                              tttAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAg                                                       >  1:779400/1‑221 (MQ=255)                                                                                                                                   gtgcTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           >  1:547739/3‑196 (MQ=255)                                                                                                                                   gtgcTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTAt                                                                      >  1:802076/3‑201 (MQ=255)                                                                                                                                   gtgcTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTg                                                                    >  1:408010/3‑203 (MQ=255)                                                                                                                                   gtgcTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGATGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAat                                                                                                          >  1:87672/3‑165 (MQ=255)                                                                                                                                    tgctTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAag                    >  1:342356‑M2/2‑248 (MQ=255)                                                                                                                                    tgctTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAag                    >  1:323455‑M2/2‑248 (MQ=255)                                                                                                                                    tgcTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAAGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAAc                                                             >  1:446296/2‑209 (MQ=255)                                                                                                                                      cttaccttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGTAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  >  1:34698‑M2/1‑246 (MQ=255)                                                                                                                                      cttaccttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  >  1:872192‑M2/1‑246 (MQ=255)                                                                                                                                      cttaccttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  >  1:201908‑M2/1‑246 (MQ=255)                                                                                                                                      cttaccttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  >  1:743831‑M2/1‑246 (MQ=255)                                                                                                                                          ccttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           >  1:298058/2‑189 (MQ=255)                                                                                                                                           cttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctg            >  1:795785‑M2/1‑241 (MQ=255)                                                                                                                                                     ttGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           <  1:232518/178‑1 (MQ=255)                                                                                                                                                     ttGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctgatgaagattg  <  1:131020‑M2/251‑21 (MQ=255)                                                                                                                                                     ttGGTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctgatgaagattg  <  1:264634‑M2/251‑21 (MQ=255)                                                                                                                                                      tGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAAt                                                                                                                                        >  1:181566/1‑116 (MQ=255)                                                                                                                                                              gtcgtaTTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAatat                                                                                                        <  1:302112/135‑1 (MQ=255)                                                                                                                                                                   aTTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctg            >  1:60434‑M2/1‑217 (MQ=255)                                                                                                                                                                         aTTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           >  1:573248/1‑158 (MQ=255)                                                                                                                                                                         aTTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           >  1:495930/1‑158 (MQ=255)                                                                                                                                                                         aTTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGc                              >  1:199259/1‑203 (MQ=255)                                                                                                                                                                         aTTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  <  1:787386‑M2/215‑5 (MQ=255)                                                                                                                                                                              aGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGc                              >  1:557585/1‑198 (MQ=255)                                                                                                                                                                                             tGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctga           >  1:658632‑M2/1‑191 (MQ=255)                                                                                                                                                                                                  aTTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGAttt                                   >  1:191502/1‑173 (MQ=255)                                                                                                                                                                                                           tATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           >  1:290042/1‑124 (MQ=255)                                                                                                                                                                                                           tATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  <  1:749558‑M2/181‑5 (MQ=255)                                                                                                                                                                                                                gggTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctgatgaagat    <  1:403199‑M2/190‑19 (MQ=255)                                                                                                                                                                                                                                     aTCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTg                                                                                                                  <  1:661443/59‑1 (MQ=255)                                                                                                                                                                                                                                     aTCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctg            <  1:540378‑M2/161‑11 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                               CCATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGCGCTTATCTTATGCGTTTTGTTATTCGCCGCATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATACCGGGTAGCGGTAAATCTTATGAGGTTGTGTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  4/76‑454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑