Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	4	289	A→T	I9I (ATA→ATT)	unknown →	Type II secretory pathway, component PulD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	4	289	0	A	T	100.0%	282.2 / NA	81	I9I (ATA→ATT)	unknown	Type II secretory pathway, component PulD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (51/30);  total (51/30)

TCTTTGCATTCAAGAAGTTGTGCCATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGCGCTTATCTTATGCGTTTTGTTATTCGCCGCATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATACCGGGTAGCGGTAAATCTTATGAGGTTGTGTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  4/54‑454                                                                                                                                                                                                                                            |                                                                                                                                                                                          tCTTTGCATTCAAGAAGTTGTGCCATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCgggc                                                                                                                                                                                     >  1:401582/1‑241 (MQ=255)         ttCAAGAAGTTGTGCCATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGa                                                                                                                                                                    <  1:857611/251‑1 (MQ=255)         ttCAAGAAGTTGTGCCATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGa                                                                                                                                                                    <  1:819547/251‑1 (MQ=255)           cAAGAAGTTGTGCCATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAgg                                                                                                                                                                  <  1:75440/251‑1 (MQ=255)                 gTTGTGCCATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAAt                                                                                                                                                                           <  1:632191/236‑1 (MQ=255)                 gTTGTGCCATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGtggttt                                                                                                                                                            >  1:523939/1‑246 (MQ=255)                       ccATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCgg                                                                                                                                                      <  1:836390/251‑1 (MQ=255)                                          ttGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTAtct                                                                                                                                   <  1:14607/251‑1 (MQ=255)                                           tGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTAtctc                                                                                                                                  <  1:77646/251‑1 (MQ=255)                                                gTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCg                                                                                                                                                                                        <  1:658925/192‑1 (MQ=255)                                                gTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTAtctc                                                                                                                                  >  1:432962/1‑246 (MQ=255)                                                gTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAgg                                                                                                                             >  1:829483/1‑251 (MQ=255)                                                 tGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAgg                                                                                                                             <  1:645551/250‑1 (MQ=255)                                                       aTTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGc                                                                                                                      <  1:302693/251‑1 (MQ=255)                                                               aaaTTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTaa                                                                                                               >  1:131072/1‑250 (MQ=255)                                                                 aTTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGa                                                                                                            >  1:148051/1‑251 (MQ=255)                                                                 aTTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGa                                                                                                            >  1:521528/1‑251 (MQ=255)                                                                 aTTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTCCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGa                                                                                                            >  1:809958/1‑251 (MQ=255)                                                                     aaCAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAatat                                                                                                        <  1:391185/251‑1 (MQ=255)                                                                         gCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAg                                                                                                    >  1:85712/1‑251 (MQ=255)                                                                         gCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAg                                                                                                    >  1:659615/1‑251 (MQ=255)                                                                                 gTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGa                                                                                                                                                                    >  1:310004/1‑179 (MQ=255)                                                                                 gTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATaa                                                                                             >  1:366247/1‑250 (MQ=255)                                                                                      gTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAAc                                                                                            >  1:840468/1‑246 (MQ=255)                                                                                      gTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAg                                                                                          <  1:361202/248‑1 (MQ=255)                                                                                      gTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACg                                                                                       >  1:760345/1‑251 (MQ=255)                                                                                             agaATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAg                                                                                 <  1:685450/250‑1 (MQ=255)                                                                                              gaATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTa                                                                               >  1:14092/1‑251 (MQ=255)                                                                                                aTGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTATCTTATGCGTTTTGTTATTCGTCGTATTCTGATTATAGGTTGAGGATTTTATGGCAATTTCGGGTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCAATGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAg                                                                             >  1:116770/1‑251 (MQ=255)                                                                                                    ggCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTa                                                                                                                                      <  1:23995/190‑1 (MQ=255)                                                                                                    ggCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAg                                                                         >  1:598379/1‑251 (MQ=255)                                                                                                         atatTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGAc                                                                                                                        >  1:540681/1‑199 (MQ=255)                                                                                                         atatTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTg                                                                    <  1:756469/251‑1 (MQ=255)                                                                                                          tatTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGt                                                                   >  1:752941/1‑251 (MQ=255)                                                                                                             tttAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTAt                                                                      >  1:51544/1‑245 (MQ=255)                                                                                                                aaaaaTGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAAt                                                                                                                                        <  1:127859/176‑1 (MQ=255)                                                                                                                aaaaaTGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTg                                                                    >  1:564071/1‑244 (MQ=255)                                                                                                                           gcgTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTaa                                                                                                               >  1:444431/1‑190 (MQ=255)                                                                                                                           gcgTTGATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           <  1:195520/226‑1 (MQ=255)                                                                                                                                gATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAg                                                                                 <  1:657494/215‑1 (MQ=255)                                                                                                                                gATTTTGCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAAGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAgg                                             >  1:444322/1‑251 (MQ=255)                                                                                                                                      gCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTgag                                                                                                                                           <  1:210130/151‑1 (MQ=255)                                                                                                                                      gCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGa                                                                                                                            >  1:506073/1‑166 (MQ=255)                                                                                                                                      gCTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAAc                                                                                            <  1:779253/198‑1 (MQ=255)                                                                                                                                       cTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAat                                                                                                          >  1:9532/1‑183 (MQ=255)                                                                                                                                       cTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGa                                      >  1:820060/1‑251 (MQ=255)                                                                                                                                       cTTTTAAGGTGGTTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGa                                      >  1:181379/1‑251 (MQ=255)                                                                                                                                                tggtTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGc                              <  1:5837/250‑1 (MQ=255)                                                                                                                                                tggtTTTAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGc                              <  1:684602/250‑1 (MQ=255)                                                                                                                                                    tttAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGttt                                                                 <  1:234879/211‑1 (MQ=255)                                                                                                                                                    tttAAGTGCTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAg                                                       >  1:779400/1‑221 (MQ=255)                                                                                                                                                         gtgcTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           >  1:547739/3‑196 (MQ=255)                                                                                                                                                         gtgcTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTAt                                                                      >  1:802076/3‑201 (MQ=255)                                                                                                                                                         gtgcTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTg                                                                    >  1:408010/3‑203 (MQ=255)                                                                                                                                                         gtgcTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGATGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAat                                                                                                          >  1:87672/3‑165 (MQ=255)                                                                                                                                                          tgctTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAag                    >  1:342356‑M2/2‑248 (MQ=255)                                                                                                                                                          tgctTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAag                    >  1:323455‑M2/2‑248 (MQ=255)                                                                                                                                                          tgcTTACCTTATGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAAGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAAc                                                             >  1:446296/2‑209 (MQ=255)                                                                                                                                                            cttaccttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGTAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  >  1:34698‑M2/1‑246 (MQ=255)                                                                                                                                                            cttaccttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  >  1:743831‑M2/1‑246 (MQ=255)                                                                                                                                                            cttaccttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  >  1:201908‑M2/1‑246 (MQ=255)                                                                                                                                                            cttaccttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  >  1:872192‑M2/1‑246 (MQ=255)                                                                                                                                                                ccttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           >  1:298058/2‑189 (MQ=255)                                                                                                                                                                 cttatGCGTTTTGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctg            >  1:795785‑M2/1‑241 (MQ=255)                                                                                                                                                                           ttGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAgg                                                                                                                                                                  >  1:735951/1‑91 (MQ=255)                                                                                                                                                                           ttGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           <  1:232518/178‑1 (MQ=255)                                                                                                                                                                           ttGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctgatgaagattg  <  1:131020‑M2/251‑21 (MQ=255)                                                                                                                                                                           ttGGTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctgatgaagattg  <  1:264634‑M2/251‑21 (MQ=255)                                                                                                                                                                            tGTTATTCGTCGTATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAAt                                                                                                                                        >  1:181566/1‑116 (MQ=255)                                                                                                                                                                                    gtcgtaTTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAatat                                                                                                        <  1:302112/135‑1 (MQ=255)                                                                                                                                                                                         aTTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctg            >  1:60434‑M2/1‑217 (MQ=255)                                                                                                                                                                                               aTTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           >  1:573248/1‑158 (MQ=255)                                                                                                                                                                                               aTTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           >  1:495930/1‑158 (MQ=255)                                                                                                                                                                                               aTTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGc                              >  1:199259/1‑203 (MQ=255)                                                                                                                                                                                               aTTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  <  1:787386‑M2/215‑5 (MQ=255)                                                                                                                                                                                                    aGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGc                              >  1:557585/1‑198 (MQ=255)                                                                                                                                                                                                                   tGGCAATTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctga           >  1:658632‑M2/1‑191 (MQ=255)                                                                                                                                                                                                                        aTTTCGGCTTATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGAttt                                   >  1:191502/1‑173 (MQ=255)                                                                                                                                                                                                                                 tATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAggg                                                                           >  1:290042/1‑124 (MQ=255)                                                                                                                                                                                                                                 tATGTGGGTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAaggg                  <  1:749558‑M2/181‑5 (MQ=255)                                                                                                                                                                                                                                      gggTATTCCGGGCAGCGGTAAATCTTATGAGGTGGTTTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATAagggtatctgatgaagat    <  1:403199‑M2/190‑19 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                          TCTTTGCATTCAAGAAGTTGTGCCATTGCTTTTAGATATTTTTGTCGGTGATAAATTTGATGAAATTAAACAGCTTTTGGGTCAGGTGCCGGAGAATGTGGCTTATATTTTAAAAATGTTCCGCGTTGATTTTGCTTTTAAGGTGGTTTTAAGCGCTTATCTTATGCGTTTTGTTATTCGCCGCATTCCGATTATAGGTTGAGGATTTTATGGCAATTTCGGCTTATGTGGGTATACCGGGTAGCGGTAAATCTTATGAGGTTGTGTCTTCGGTGATTGTTGAGAATTATCTCAAAGGAAGACGCATTGTAACGAATATTGAGGGAATAACAGACGAAAAAGTAAGGGAGTATTGTTTAAACAAAAAGAATGCCAAGGAAGACGATTTAGGGCAATTAATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  4/54‑454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑