Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	3	1,970	C→T	G351G (GGC→GGT)	unknown →	Type II secretory pathway, component PulD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	3	1,970	0	C	T	100.0%	233.7 / NA	70	G351G (GGC→GGT)	unknown	Type II secretory pathway, component PulD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/35);  total (35/35)

CTATTGAATATCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGCGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  3/1744‑2032                                                                                                                                                                                                                                   |                                                                                                                                                                                                                     ccattgaatgccacTCAAGCGATGAAATTTTTGCCTAATTGCCGACGATTAAAGCCGATAATATTGAAATAGAAATTCAGCAACAACCATCGAATTTTCTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCt                                                                                                                                                                                              <  1:268818/238‑1 (MQ=21) ccaTTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTCAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATttcttc                                                                                                                                                                                                >  1:423691/3‑249 (MQ=255)           ccGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGttttt                                                                                                                                                                                    >  1:74522/2‑251 (MQ=255)           ccGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGttttt                                                                                                                                                                                    >  1:774484/2‑251 (MQ=255)           ccGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCAATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGttttt                                                                                                                                                                                    >  1:441150/2‑251 (MQ=255)             gCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTa                                                                                                                                                                                  >  1:442679/1‑251 (MQ=255)                      gtgtAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGaaaa                                                                                                                                                                         >  1:458082/1‑251 (MQ=255)                                          ccGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgac                                                                                                                                                     <  1:376424‑M1/251‑4 (MQ=255)                                          ccGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgac                                                                                                                                                     <  1:608949‑M1/251‑4 (MQ=255)                                          ccGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgac                                                                                                                                                     <  1:800716‑M1/251‑4 (MQ=255)                                               gaTTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgac                                                                                                                                                     <  1:371293‑M1/246‑4 (MQ=255)                                                aTTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatat                                                                                                                                               <  1:265297‑M1/251‑10 (MQ=255)                                                aTTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatat                                                                                                                                               <  1:123915‑M1/251‑10 (MQ=255)                                                    aaGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtg                                                                                                                                           <  1:411575‑M1/251‑14 (MQ=255)                                                      gCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagat                                                                                                                                                 >  1:831889‑M1/1‑236 (MQ=255)                                                        cGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtg                                                                                                                                           >  1:847688‑M1/1‑234 (MQ=255)                                                         gATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTaa                                                                                                                                                                                 <  1:791817/208‑1 (MQ=255)                                                          ataataTTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAAc                                                                                                                                                                                >  1:598548/1‑208 (MQ=255)                                                          ataataTTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATAc                                                                                                                                                           <  1:767722/229‑1 (MQ=255)                                                             atatTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgac                                                                                                                                                     >  1:786945‑M1/1‑229 (MQ=255)                                                             atatTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgac                                                                                                                                                     >  1:207262‑M1/1‑229 (MQ=255)                                                                   atatAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtg                                                                                                                            <  1:9866‑M1/251‑29 (MQ=255)                                                                                       cTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatattgtg                                                                                                                                           >  1:208128‑M1/1‑203 (MQ=255)                                                                                       cTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgac                                                                                                                                                     >  1:210037‑M1/1‑203 (MQ=255)                                                                                         aTCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttga                                                                                                      >  1:897988‑M1/1‑201 (MQ=255)                                                                                         aTCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtc                                                                                                                   <  1:735103‑M1/238‑38 (MQ=255)                                                                                              aTTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaag                                                                                                 >  1:47361‑M1/1‑196 (MQ=255)                                                                                                        gACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAAc                                                                                                                                                                                <  1:598161/162‑1 (MQ=255)                                                                                                        gACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatg                                                                                       >  1:631333‑M1/1‑186 (MQ=255)                                                                                                        gACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtcta                                                                                                                 <  1:645917‑M1/225‑40 (MQ=255)                                                                                                        gACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgac                                                                                                                                                     <  1:356024‑M1/189‑4 (MQ=255)                                                                                                           ggATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAATAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtag                                                                                    >  1:92644‑M1/1‑183 (MQ=255)                                                                                                           ggATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgac                                                                                                                                                     >  1:126598‑M1/1‑183 (MQ=255)                                                                                                            gATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagg                                                                                   >  1:60513‑M1/1‑182 (MQ=255)                                                                                                            gATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtg                                                                                                                                           >  1:710266‑M1/1‑182 (MQ=255)                                                                                                             aTACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCAt                                                                                                                                                                                                      >  1:216121/1‑135 (MQ=255)                                                                                                              tACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttac                                                                                                                                    >  1:740583‑M1/1‑180 (MQ=255)                                                                                                              tACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtg                                                                                                                                           <  1:573317‑M1/193‑14 (MQ=255)                                                                                                                             atcTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCaaaa                                                                                                                                                                  <  1:858884/155‑1 (MQ=255)                                                                                                                                    acacTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaac                                                           <  1:828510‑M1/251‑94 (MQ=255)                                                                                                                                    acacTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTCTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaac                                                           <  1:639394‑M1/251‑94 (MQ=255)                                                                                                                                     cacTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaacc                                                          <  1:119271‑M1/251‑95 (MQ=255)                                                                                                                                      acTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaacca                                                         <  1:283417‑M1/251‑96 (MQ=255)                                                                                                                                       cTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgac                                                                                                                                                     <  1:700016‑M1/158‑4 (MQ=255)                                                                                                                                           taAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctc                                                    <  1:21991‑M1/251‑101 (MQ=255)                                                                                                                                             aaaCGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaac                                                           >  1:882242‑M1/1‑149 (MQ=255)                                                                                                                                               aCGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatg                                                <  1:871823‑M1/251‑105 (MQ=255)                                                                                                                                                cgcgACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgt                                               <  1:796521‑M1/251‑106 (MQ=255)                                                                                                                                                  cgACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgtta                                             <  1:624089‑M1/251‑108 (MQ=255)                                                                                                                                                         gTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcggg                                       >  1:315815‑M1/1‑137 (MQ=255)                                                                                                                                                          tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcgggca                                     >  1:321878‑M1/1‑136 (MQ=255)                                                                                                                                                          tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcgggc                                      <  1:251811‑M1/250‑115 (MQ=255)                                                                                                                                                          tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgt                                               <  1:92661‑M1/241‑106 (MQ=255)                                                                                                                                                          tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatat                                                                                                                                               >  1:119379‑M1/1‑136 (MQ=255)                                                                                                                                                               gATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCa                                                                                                                                                                                                       <  1:120165/84‑1 (MQ=255)                                                                                                                                                               gATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCaaaaa                                                                                                                                                                 >  1:435792/1‑122 (MQ=255)                                                                                                                                                               gATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcgggcatttt                                 >  1:791560‑M1/1‑131 (MQ=255)                                                                                                                                                               gATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtga                                                                                                                                          <  1:472060‑M1/145‑15 (MQ=255)                                                                                                                                                                aTGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcgggcattttta                               >  1:18069‑M1/1‑130 (MQ=255)                                                                                                                                                                aTGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaact                                                                                                                       <  1:595573‑M1/163‑34 (MQ=255)                                                                                                                                                                   tAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaagccatgctcgatgttattcgggcatttttattt                            <  1:721810‑M1/251‑125 (MQ=255)                                                                                                                                                                      gtgtAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtg                                                                                                                                           >  1:645560‑M1/1‑124 (MQ=255)                                                                                                                                                                        gtAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatag                                                                                                                                             <  1:602292‑M1/133‑12 (MQ=255)                                                                                                                                                                                     aTGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcgggcatttttattttttgcgcgtacctaaatt          >  1:212832‑M1/1‑109 (MQ=255)                                                                                                                                                                                        tgATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcgggcatttttattttttgcgcgtacctaaatttaa       >  1:137407‑M1/1‑106 (MQ=255)                                                                                                                                                                                        tgATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcgggcatttttattttttgcgcgtacc                <  1:57031‑M1/242‑137 (MQ=255)                                                                                                                                                                                             gTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcgggcatttttattttttgcgcgtacctaaatttaatcgag  >  1:717953‑M1/1‑101 (MQ=255)                                                                                                                                                                                             gTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcgggcatttttattttttgcgcgtacctaaatttaatcgag  >  1:322230‑M1/1‑101 (MQ=255)                                                                                                                                                                                             gTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaaccatgctcgatgttattcgggcatttttattttttgcgcgtacctaaat           <  1:275550‑M1/242‑142 (MQ=255)                                                                                                                                                                                                                 aaataaaataACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAgacagatatagtgattttactccaagtgaaacttgtctaactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcccggtaaac                                                           >  1:587545‑M1/1‑81 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                     CTATTGAATATCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGCGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  3/1744‑2032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑