Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	3	1,745	T→C	S276S (TCT→TCC)	unknown →	Type II secretory pathway, component PulD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	3	1,745	0	T	C	100.0%	207.2 / NA	60	S276S (TCT→TCC)	unknown	Type II secretory pathway, component PulD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/27);  total (33/27)

ACTTCTTTCAGGAAAACTCGGGATTAATATAGGCGTTAAGCAGAACTATGAAAACTTTATAACAGTAAATACAGGTAATCTTGATGCTATGATTGAACTTTTTCGTACAGATAGCCGATTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCTATTGAATATCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAAC  >  3/1502‑1963                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                           ttaatcaatctccgacacttataaaacgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAat                                                                                                                                                                                                                     >  1:184977‑M1/119‑251 (MQ=255)   aatcaatctccgacacttataaaacgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGaatac                                                                                                                                                                                                                   >  1:91773‑M1/117‑250 (MQ=255)   aatcaatctccgacacttataaaacgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGaatac                                                                                                                                                                                                                   >  1:641971‑M1/117‑250 (MQ=255)   aatcaatctccgacacttataaaacgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGaatac                                                                                                                                                                                                                   >  1:463166‑M1/117‑250 (MQ=255)           tccgacacttataaaacgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAg                                                                                                                                                                                                           >  1:567686‑M1/109‑251 (MQ=255)           tccgacacttataaaacgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAg                                                                                                                                                                                                           >  1:604607‑M1/109‑251 (MQ=255)                  cttataaaacgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAg                                                                                                                                                                                                           <  1:562704‑M1/143‑1 (MQ=255)                  cttataaaacgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTa                                                                                                                                                                                                    <  1:901560‑M1/150‑1 (MQ=255)                   ttataaaacgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTaa                                                                                                                                                                                                   >  1:111505‑M1/101‑251 (MQ=255)                      taaaacgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAAttt                                                                                                                                                                                                >  1:11707‑M1/98‑251 (MQ=255)                          acgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCgg                                                                                                                                                                                                        <  1:276949‑M1/146‑1 (MQ=255)                           cgcgacattgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGAc                                                                                                                                                                                           >  1:257324‑M1/93‑251 (MQ=255)                                  ttgtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGc                                                                                                                                                                                    >  1:820997‑M1/86‑251 (MQ=255)                                    gtcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCTGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCcg                                                                                                                                                                                  <  1:519034‑M1/168‑1 (MQ=255)                                     tcacagatgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCcga                                                                                                                                                                                 <  1:723115‑M1/169‑1 (MQ=255)                                           atgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGaatac                                                                                                                                                                                                                   >  1:787441‑M1/77‑210 (MQ=255)                                           atgtaagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTa                                                                                                                                                                           <  1:680278‑M1/175‑1 (MQ=255)                                               aagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGc                                                                                                                                                                       >  1:853130‑M1/73‑251 (MQ=255)                                               aagtgtaaaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGc                                                                                                                                                                       >  1:447482‑M1/73‑251 (MQ=255)                                                      aaaaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGataat                                                                                                                                                                >  1:652310‑M1/66‑251 (MQ=255)                                                        aaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAatat                                                                                                                                                              >  1:716587‑M1/64‑251 (MQ=255)                                                        aaatggcgatgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAatat                                                                                                                                                              >  1:753040‑M1/64‑251 (MQ=255)                                                                 tgtgatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATaaa                                                                                                                                                     >  1:542047‑M1/55‑251 (MQ=255)                                                                    gatagttttgggtggactggccgaaaacaagataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAGTTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAAt                                                                                                                                                  <  1:623800‑M1/200‑1 (MQ=255)                                                                                                 agataactgaaggcgaaacaggTTTCACGTTGTAAGCTCTTCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAatat                                                                                                                                                              >  1:428899‑M1/23‑210 (MQ=255)                                                                                                     aactgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGa                                                                                                                 <  1:405378‑M1/233‑1 (MQ=255)                                                                                                      actgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGAt                                                                                                                <  1:301654‑M1/234‑1 (MQ=255)                                                                                                        tgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATAc                                                                                                              >  1:266106‑M1/16‑251 (MQ=255)                                                                                                        tgaaggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATAc                                                                                                              >  1:506595‑M1/16‑251 (MQ=255)                                                                                                            ggcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGgt                                                                                                          <  1:245335‑M1/240‑1 (MQ=255)                                                                                                             gcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAgg                                                                                                           >  1:106492‑M1/11‑249 (MQ=255)                                                                                                             gcgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAgg                                                                                                           >  1:407303‑M1/11‑249 (MQ=255)                                                                                                              cgaaacaggTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGgtg                                                                                                         >  1:701868‑M1/10‑250 (MQ=255)                                                                                                                      gTTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTaat                                                                                                    >  1:379028‑M1/2‑247 (MQ=255)                                                                                                                              ttGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGaca                                                                                        >  1:657609/1‑251 (MQ=255)                                                                                                                                     ctctCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATaa                                                                                 <  1:768913/251‑1 (MQ=255)                                                                                                                                       ctcCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATaaaa                                                                               <  1:216722/251‑1 (MQ=255)                                                                                                                                       ctcCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATaaaa                                                                               >  1:603086/1‑251 (MQ=255)                                                                                                                                        tcCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAAc                                                                              <  1:707189/251‑1 (MQ=255)                                                                                                                                                        aaaTCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTg                                                                                                                                                            >  1:414478/1‑157 (MQ=255)                                                                                                                                                              ggCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACGACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAg                                                        >  1:454688/1‑251 (MQ=255)                                                                                                                                                               gCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAg                                                        <  1:523530/250‑1 (MQ=255)                                                                                                                                                                       ggCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTaaaaa                                                <  1:278498/250‑1 (MQ=255)                                                                                                                                                                           aTTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATaaaa                                                                               >  1:737541/1‑215 (MQ=255)                                                                                                                                                                                  cGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGc                                                                                                                                                                       <  1:717171/120‑1 (MQ=255)                                                                                                                                                                                      ggCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAgg                                                                                                           >  1:788929/1‑176 (MQ=255)                                                                                                                                                                                       gCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATaa                                                                                 <  1:274985/201‑1 (MQ=255)                                                                                                                                                                                       gCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGAt                                                             <  1:590501/221‑1 (MQ=255)                                                                                                                                                                                            gATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATaaaa                                                                               <  1:699330/198‑1 (MQ=255)                                                                                                                                                                                             aTGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTtggt                         <  1:516623/251‑1 (MQ=255)                                                                                                                                                                                              tGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTtggt                         <  1:233933/250‑1 (MQ=255)                                                                                                                                                                                                   cTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTc                              <  1:622360/240‑1 (MQ=255)                                                                                                                                                                                                     aTTCTTGGCTCGGTTACTTATGGTAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTtggt                         <  1:178328/243‑1 (MQ=255)                                                                                                                                                                                                       tCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAatac                                                                                                                                                                                                                   <  1:585392/55‑2 (MQ=255)                                                                                                                                                                                                               cGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTg                                 >  1:893448/1‑225 (MQ=255)                                                                                                                                                                                                                 gTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAg                                                        <  1:787049/200‑1 (MQ=255)                                                                                                                                                                                                                 gTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGaaaataaaat     >  1:772789/1‑251 (MQ=255)                                                                                                                                                                                                                  ttACTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTaaaaa                                                >  1:826039/1‑207 (MQ=255)                                                                                                                                                                                                                    aCTTATGATAGAAATGGTCGTCCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAAc  <  1:395749/251‑1 (MQ=255)                                                                                                                                                                                                                                    gtcgtcCGGTGCAGTCCATTGAATACCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAg                                                        <  1:291222/181‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                           ACTTCTTTCAGGAAAACTCGGGATTAATATAGGCGTTAAGCAGAACTATGAAAACTTTATAACAGTAAATACAGGTAATCTTGATGCTATGATTGAACTTTTTCGTACAGATAGCCGATTTCACGTTGTAAGCTCTCCGACAATTCGCGTTAAATCAGGCTCAAAGGGCAATTTTAGCGTTGGCTCTGATGTACCTATTCTTGGCTCGGTTACTTATGATAGAAATGGTCGTCCGGTGCAGTCTATTGAATATCGCTCAAGCGGTGTAATTTTTGACATATTGCCGACGATTAAAGCCGATAATATTGATATAAAAATTCAGCAACAACTATCTAATTTTGTTAAGACGGATACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAAC  >  3/1502‑1963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑